LOCUS       BBa_K1491034            3495 bp    DNA     linear       16-Feb-2025
DEFINITION  Constitutive T7 RNAP with YFPH
ACCESSION   BBa_K1491034
VERSION     BBa_K1491034.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23115
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    2711..2728
                     /label=6x His Tag
     CDS             62..2728
                     /label=BBa_K1491030
     misc_feature    2741..2744
                     /label=conserved
     RBS             2737..2748
                     /label=BBa_B0034
     misc_feature    2755..3471
                     /label=BBa_K864100
     misc_feature    3472..3489
                     /label=6x His
     CDS             2755..3495
                     /label=BBa_K1491004
     misc_feature    2737..3495
                     /label=BBa_K1491005
ORIGIN
        1 tttatagcta gctcagccct tggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg ctgctatccc
      121 gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt tggcccttga
      181 gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa
      241 agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc tactccctaa
      301 gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc
      361 gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa
      421 gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg tagcaagcgc
      481 aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa
      541 gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg tctacaagaa
      601 agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg gtggcgaggc
      661 gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca tcgagatgct
      721 cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag taggtcaaga
      781 ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc
      841 gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg
      901 cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca
      961 cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt acaaagcgat
     1021 taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg tcgccaacgt
     1081 aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc gtgaagaact
     1141 cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc
     1201 tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca gccttgagtt
     1261 catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc cttacaacat
     1321 ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta acgatatgac
     1381 caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt actactggct
     1441 gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa
     1501 gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac tggagaacac
     1561 ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg agtacgctgg
     1621 ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg acgggtcttg
     1681 ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa
     1741 cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga aagtcaacga
     1801 gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg tgaccgatga
     1861 gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg ctggtcaatg
     1921 gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc tggcttacgg
     1981 gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc cagctattga
     2041 ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca tggctaagct
     2101 gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga actggcttaa
     2161 gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag agattcttcg
     2221 caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc aggaatacaa
     2281 gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct tacagcctac
     2341 cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg gtatcgctcc
     2401 taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt gggcacacga
     2461 gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca ttccggctga
     2521 cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg agtcttgtga
     2581 tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc aattggacaa
     2641 aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag agtcggactt
     2701 cgcgttcgcg catcatcacc accatcacta ctagagaaag aggagaaata ctagatggtt
     2761 agcaagggcg aagaactttt tacaggcgta gtaccgatct tagttgaatt agacggcgac
     2821 gttaacggtc ataagtttag cgtgagcggt gagggtgaag gtgacgcaac ttacggcaag
     2881 ctgaccctga agctgatttg cacgacgggt aagctgccgg tcccgtggcc taccctggtc
     2941 acgaccttgg gttatggcgt tcagtgtttc gcgcgttatc cggaccacat gaaacaacac
     3001 gatttcttta agagcgcgat gccagaaggc tatgtgcagg agcgtacgat ctttttcaaa
     3061 gacgacggta actacaagac gcgtgccgaa gtcaaattcg aaggcgacac cctggtgaat
     3121 cgcattgagc tgaagggtat tgatttcaaa gaggatggca atatcctggg tcacaagctg
     3181 gagtacaatt acaattccca caacgtttac atcaccgcag ataaacagaa aaatggcatc
     3241 aaagcgaatt tcaaaatccg tcacaacatt gaggacggtg gtgttcaact ggcggatcat
     3301 taccagcaaa acaccccgat tggtgacggt ccggtcctgt tgccggataa ccattatctg
     3361 tcttaccaaa gcaaactgag caaagatccg aacgagaagc gcgaccacat ggtgctgctg
     3421 gagtttgtga ccgctgccgg tattaccctg ggtatggatg agctgtataa acatcatcac
     3481 caccatcact aataa
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