BBa_K1491035
type: ComponentDefinition
persistent identity: BBa K1491035
display id: BBa_K1491035
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive ER Intein T7 RNAP with YFPH
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the ER Intein is BBa_K1491024, the YFP is BBa_K864100
owned by: james
owned by: myers
mutable description: T7 RNA polymerase that contains the estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP.
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constitutive ER Intein T7 RNAP with YFP for quantification of T7 RNAP
star: false
creator: Danielle Peters
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1491005
component: BBa_K1491025
component: BBa_B0034
component: BBa_J23115
sequence annotation: BBa_J23115
sequence annotation: BBa_B0034
sequence annotation: BBa_K1491025
sequence annotation: BBa_K1491005
sequence: https://synbiohub.org/public/igem/BBa_K1491035_sequence/1

BBa_K1491005
type: Component
persistent identity: component2414176
display id: component2414176
version: 1
top level: BBa_K1491035
definition: BBa_K1491005
access: public

BBa_K1491025
type: Component
persistent identity: component2414170
display id: component2414170
version: 1
top level: BBa_K1491035
definition: BBa_K1491025
access: public

BBa_B0034
type: Component
persistent identity: component2414162
display id: component2414162
version: 1
top level: BBa_K1491035
definition: BBa_B0034
access: public

BBa_J23115
type: Component
persistent identity: component2414160
display id: component2414160
version: 1
top level: BBa_K1491035
definition: BBa_J23115
access: public

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2414160
display id: annotation2414160
version: 1
top level: BBa_K1491035
location: https://synbiohub.org/public/igem/BBa_K1491035/annotation2414160/range2414160/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K1491035/annotation2414160/range2414160/1
type: Range
persistent identity: range2414160
display id: range2414160
version: 1
top level: BBa_K1491035
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2414162
display id: annotation2414162
version: 1
top level: BBa_K1491035
location: https://synbiohub.org/public/igem/BBa_K1491035/annotation2414162/range2414162/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491035/annotation2414162/range2414162/1
type: Range
persistent identity: range2414162
display id: range2414162
version: 1
top level: BBa_K1491035
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1491025
type: SequenceAnnotation
persistent identity: annotation2414170
display id: annotation2414170
version: 1
top level: BBa_K1491035
location: https://synbiohub.org/public/igem/BBa_K1491035/annotation2414170/range2414170/1
component: BBa_K1491025

https://synbiohub.org/public/igem/BBa_K1491035/annotation2414170/range2414170/1
type: Range
persistent identity: range2414170
display id: range2414170
version: 1
top level: BBa_K1491035
direction: direction/reverse
start: 62
end: 4319
orientation: inline

BBa_K1491005
type: SequenceAnnotation
persistent identity: annotation2414176
display id: annotation2414176
version: 1
top level: BBa_K1491035
location: https://synbiohub.org/public/igem/BBa_K1491035/annotation2414176/range2414176/1
component: BBa_K1491005

https://synbiohub.org/public/igem/BBa_K1491035/annotation2414176/range2414176/1
type: Range
persistent identity: range2414176
display id: range2414176
version: 1
top level: BBa_K1491035
direction: direction/reverse
start: 4328
end: 5086
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

BBa_K1491005
type: ComponentDefinition
persistent identity: BBa K1491005
display id: BBa_K1491005
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-SuperYFP2-His
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: RBS is BBa_B0034 and SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
mutable description: SuperYFP2 with 6x His tag(BBa_K1491004) and a strong RBS (BBa_B0034). It can be used as a reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: RBS+YFP+His
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1491004
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1491004
sequence: https://synbiohub.org/public/igem/BBa_K1491005_sequence/1

BBa_K1491004
type: Component
persistent identity: component2380100
display id: component2380100
version: 1
top level: BBa_K1491005
definition: BBa_K1491004
access: public

BBa_B0034
type: Component
persistent identity: component2380097
display id: component2380097
version: 1
top level: BBa_K1491005
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380097
display id: annotation2380097
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380097/range2380097/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491005/annotation2380097/range2380097/1
type: Range
persistent identity: range2380097
display id: range2380097
version: 1
top level: BBa_K1491005
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1491004
type: SequenceAnnotation
persistent identity: annotation2380100
display id: annotation2380100
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
component: BBa_K1491004

https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
type: Range
persistent identity: range2380100
display id: range2380100
version: 1
top level: BBa_K1491005
direction: direction/reverse
start: 19
end: 759
orientation: inline

BBa_K1491025
type: ComponentDefinition
persistent identity: BBa K1491025
display id: BBa_K1491025
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA Polymerase with ER Intein
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: T7 RNA polymerase is BBa_K145001 and intein is BBa_K1491024
owned by: james
owned by: myers
mutable description: T7 RNA polymerase with estrogen receptor intein embedded. Non functional with intein. When estrogen is introduced to the system, the intein splices out and links pieces of T7 RNA polymerase together, creating the functional version of the protein.
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Estrogen sensitive T7 RNA polymerase
star: false
creator: Danielle Peters
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1491022
component: BBa_K1491023
component: BBa_K1491024
sequence annotation: BBa_K1491022
sequence annotation: BBa_K1491024
sequence annotation: BBa_K1491023
sequence: https://synbiohub.org/public/igem/BBa_K1491025_sequence/1

BBa_K1491022
type: Component
persistent identity: component2384093
display id: component2384093
version: 1
top level: BBa_K1491025
definition: BBa_K1491022
access: public

BBa_K1491023
type: Component
persistent identity: component2384099
display id: component2384099
version: 1
top level: BBa_K1491025
definition: BBa_K1491023
access: public

BBa_K1491024
type: Component
persistent identity: component2384097
display id: component2384097
version: 1
top level: BBa_K1491025
definition: BBa_K1491024
access: public

BBa_K1491022
type: SequenceAnnotation
persistent identity: annotation2384093
display id: annotation2384093
version: 1
top level: BBa_K1491025
location: https://synbiohub.org/public/igem/BBa_K1491025/annotation2384093/range2384093/1
component: BBa_K1491022

https://synbiohub.org/public/igem/BBa_K1491025/annotation2384093/range2384093/1
type: Range
persistent identity: range2384093
display id: range2384093
version: 1
top level: BBa_K1491025
direction: direction/reverse
start: 1
end: 1473
orientation: inline

BBa_K1491024
type: SequenceAnnotation
persistent identity: annotation2384097
display id: annotation2384097
version: 1
top level: BBa_K1491025
location: https://synbiohub.org/public/igem/BBa_K1491025/annotation2384097/range2384097/1
component: BBa_K1491024

https://synbiohub.org/public/igem/BBa_K1491025/annotation2384097/range2384097/1
type: Range
persistent identity: range2384097
display id: range2384097
version: 1
top level: BBa_K1491025
direction: direction/reverse
start: 1482
end: 3056
orientation: inline

BBa_K1491023
type: SequenceAnnotation
persistent identity: annotation2384099
display id: annotation2384099
version: 1
top level: BBa_K1491025
location: https://synbiohub.org/public/igem/BBa_K1491025/annotation2384099/range2384099/1
component: BBa_K1491023

https://synbiohub.org/public/igem/BBa_K1491025/annotation2384099/range2384099/1
type: Range
persistent identity: range2384099
display id: range2384099
version: 1
top level: BBa_K1491025
direction: direction/reverse
start: 3065
end: 4258
orientation: inline

BBa_K1491004
type: ComponentDefinition
persistent identity: BBa K1491004
display id: BBa_K1491004
version: 1
was generated by: iGEM to SBOL conversion
description: SuperYFP2-His
created: 2014-07-14T11:00:00Z
modified: 2016-01-21T02:28:40Z
mutable provenance: SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
mutable description: SuperYFP2 (BBa_K864100) with 6x His tag for protein purification.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 4206
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: YFP+His
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K864100
sequence annotation: 6x His
sequence: https://synbiohub.org/public/igem/BBa_K1491004_sequence/1

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2380094
display id: annotation2380094
version: 1
top level: BBa_K1491004
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1
type: Range
persistent identity: range2380094
display id: range2380094
version: 1
top level: BBa_K1491004
direction: direction/forward
start: 1
end: 717
orientation: inline

6x His
type: SequenceAnnotation
persistent identity: annotation2380095
display id: annotation2380095
version: 1
top level: BBa_K1491004
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1
type: Range
persistent identity: range2380095
display id: range2380095
version: 1
top level: BBa_K1491004
direction: direction/forward
start: 718
end: 735
orientation: inline

BBa_K1491022
type: ComponentDefinition
persistent identity: BBa K1491022
display id: BBa_K1491022
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminus of T7 RNA Polymerase
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: BBa_K145001
owned by: james
owned by: myers
mutable description: Codes for the N-terminus of T7 RNA polymerase (BBa_K145001). This was used to create a T7 polymerase-intein complex.
discontinued: false
dominant: false
experience: experience/None
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to insert the intein into T7 RNA polymerase, we needed both the N and C terms.
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1491022_sequence/1

BBa_K1491023
type: ComponentDefinition
persistent identity: BBa K1491023
display id: BBa_K1491023
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminus of T7 RNA Polymerase
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: BBa_K145001
owned by: james
owned by: myers
mutable description: Codes for the N-terminus of T7 RNA polymerase (BBa_K145001). This was used to create a T7 polymerase-intein complex.
discontinued: false
dominant: false
experience: experience/None
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to insert the intein into T7 RNA polymerase, we needed both the N and C terms.
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1491023_sequence/1

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2384063
display id: annotation2384063
version: 1
top level: BBa_K1491023
location: https://synbiohub.org/public/igem/BBa_K1491023/annotation2384063/range2384063/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1491023/annotation2384063/range2384063/1
type: Range
persistent identity: range2384063
display id: range2384063
version: 1
top level: BBa_K1491023
direction: direction/forward
start: 1177
end: 1194
orientation: inline

BBa_K1491024
type: ComponentDefinition
persistent identity: BBa K1491024
display id: BBa_K1491024
version: 1
was generated by: iGEM to SBOL conversion
description: ER Intein
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: Chong, Shaorong, Kay S. Williams, Chad Wotkowicz, and Ming-Qun Xu. "Modulation of Protein Splicing of the Saccharomyces Cerevisiae Vacuolar Membrane ATPase Intein." Journal of Biological Chemistry 273.17 (1998): 10567-0577.
owned by: james
owned by: myers
mutable description: The intein contains the human E2 estrogen ligand binding domain. When estrogen binds to the receptor, the intein splices out of the protein and joins the N and C terms of the protein, thereby activating it. This allows for an estrogen sensitive protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Estrogen sensitive intein
star: false
creator: Danielle Peters
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: N term Intein
sequence annotation: Estrogen Receptor
sequence annotation: C term Intein
sequence: https://synbiohub.org/public/igem/BBa_K1491024_sequence/1

N term Intein
type: SequenceAnnotation
persistent identity: annotation2383955
display id: annotation2383955
version: 1
top level: BBa_K1491024
location: https://synbiohub.org/public/igem/BBa_K1491024/annotation2383955/range2383955/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1491024/annotation2383955/range2383955/1
type: Range
persistent identity: range2383955
display id: range2383955
version: 1
top level: BBa_K1491024
direction: direction/forward
start: 1
end: 615
orientation: inline

Estrogen Receptor
type: SequenceAnnotation
persistent identity: annotation2383956
display id: annotation2383956
version: 1
top level: BBa_K1491024
location: https://synbiohub.org/public/igem/BBa_K1491024/annotation2383956/range2383956/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1491024/annotation2383956/range2383956/1
type: Range
persistent identity: range2383956
display id: range2383956
version: 1
top level: BBa_K1491024
direction: direction/forward
start: 616
end: 1373
orientation: inline

C term Intein
type: SequenceAnnotation
persistent identity: annotation2383957
display id: annotation2383957
version: 1
top level: BBa_K1491024
location: https://synbiohub.org/public/igem/BBa_K1491024/annotation2383957/range2383957/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1491024/annotation2383957/range2383957/1
type: Range
persistent identity: range2383957
display id: range2383957
version: 1
top level: BBa_K1491024
direction: direction/forward
start: 1374
end: 1575
orientation: inline

https://synbiohub.org/public/igem/BBa_K1491035_sequence/1
type: Sequence
persistent identity: BBa K1491035 sequence
display id: BBa_K1491035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatagctagctcagcccttggtacaatgctagctactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttactagagtgctttgccaagggtaccaatgttttaatggcggatgggtctattgaatgtattgagaacattgaggttggtaataaggtcatgggtaaagatggcagacctcgtgaggtaattaaattgcccagaggaagtgaaactatgtacagtgtcgtgcagaaaagtcaacacagagcccataaaagtgactcaagtcgtgaaatgccagaattactcaagttcacgtgtaatgcgactcatgagttggttgttagaacacctcgtagtgtccgccgtttgtctcgtaccattaagggtgtcgaatattttgaagttattacttttgagatgggtcaaaagaaggcccccgacggtagaattgttgagcttgtcaaggaagtttcaaagagctatccagtatccgagggtcctgagagagctaacgaattggtagaatcctatagaaaggcttcaaataaagc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23115_sequence/1
type: Sequence
persistent identity: BBa J23115 sequence
display id: BBa_J23115_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatagctagctcagcccttggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491005_sequence/1
type: Sequence
persistent identity: BBa K1491005 sequence
display id: BBa_K1491005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491025_sequence/1
type: Sequence
persistent identity: BBa K1491025 sequence
display id: BBa_K1491025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttactagagtgctttgccaagggtaccaatgttttaatggcggatgggtctattgaatgtattgagaacattgaggttggtaataaggtcatgggtaaagatggcagacctcgtgaggtaattaaattgcccagaggaagtgaaactatgtacagtgtcgtgcagaaaagtcaacacagagcccataaaagtgactcaagtcgtgaaatgccagaattactcaagttcacgtgtaatgcgactcatgagttggttgttagaacacctcgtagtgtccgccgtttgtctcgtaccattaagggtgtcgaatattttgaagttattacttttgagatgggtcaaaagaaggcccccgacggtagaattgttgagcttgtcaaggaagtttcaaagagctatccagtatccgagggtcctgagagagctaacgaattggtagaatcctatagaaaggcttcaaataaagcttattttgagtggactattgaggccagggatctttctctgttgggttcccatgttcgtaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491004_sequence/1
type: Sequence
persistent identity: BBa K1491004 sequence
display id: BBa_K1491004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491022_sequence/1
type: Sequence
persistent identity: BBa K1491022 sequence
display id: BBa_K1491022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491023_sequence/1
type: Sequence
persistent identity: BBa K1491023 sequence
display id: BBa_K1491023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgcatcatcaccaccatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491024_sequence/1
type: Sequence
persistent identity: BBa K1491024 sequence
display id: BBa_K1491024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgctttgccaagggtaccaatgttttaatggcggatgggtctattgaatgtattgagaacattgaggttggtaataaggtcatgggtaaagatggcagacctcgtgaggtaattaaattgcccagaggaagtgaaactatgtacagtgtcgtgcagaaaagtcaacacagagcccataaaagtgactcaagtcgtgaaatgccagaattactcaagttcacgtgtaatgcgactcatgagttggttgttagaacacctcgtagtgtccgccgtttgtctcgtaccattaagggtgtcgaatattttgaagttattacttttgagatgggtcaaaagaaggcccccgacggtagaattgttgagcttgtcaaggaagtttcaaagagctatccagtatccgagggtcctgagagagctaacgaattggtagaatcctatagaaaggcttcaaataaagcttattttgagtggactattgaggccagggatctttctctgttgggttcccatgttcgtaaagctacctaccagacttacgctccaattctttatgagaatgaccactttttcgactacatgcaaaaaagtaagtttcatctcaccattgaaggtccatctaagaagaacagcctggccttgtccctgacggccgaccagatggtcagtgccttgttggatgctgagccccccatactctattccgagtatgatcctaccagacccttcagtgaggcttcgatgatgggcttactgaccaacctggcagacagggagctggttcacatgatcaactgggcgaagagggtgccaggctttgtggatttgaccctccatgatcaggtccaccttctcgaatgtgcctggctagagatcctgatgattggtctcgtctggcgctccatggagcacccagggaagctactgtttgctcctaacttgctcttggacaggaaccagggaaaatgtgtagagggcatggtggagatcttcgacatgctgctggctacatcatctcggttccgcatgatgaatctccagggagaggagtttgtgtgcctcaaatctattattttgcttaattctggagtgtacacatttctgtccagcaccctgaagtctctggaagagaaggaccatatccaccgagtcctggacaagatcacagacactttgatccacctgatggccaaggcaggcctgaccctccagcagcagcaccagcggctggcccagctcctcctcatcctctcccacatcaggcacatgagtaacaaaggcatggagcatctgtacagcatgaagtgcaagaacgtggtgcccctctatgacctgctgctggagatgctggacgcccaccgcctacatgcgcccaccggtgatgttttgcttaacgttctttctaagtgtgccggctctaaaaaattcaggcctgctcccgtcgctacttttgtacgtgagtgccagggattttatttcgagttacaagaattaaaggaaaacgattattacggaattaccttatctgatgattctgatcatcagtttttgcttgccaaccaggttgtcgtccataat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z