##gff-version 3
##sequence-region BBa_K1491036 1 5086
BBa_K1491036	.	engineered_region	4328	5086	.	+	0	ID=BBa_K1491005;Name=BBa_K1491005
BBa_K1491036	.	CDS	4346	5086	.	+	0	ID=BBa_K1491004;Name=BBa_K1491004;Parent=BBa_K1491005
BBa_K1491036	.	engineered_region	4346	5062	.	+	0	ID=annotation2380094;Name=BBa_K864100;Parent=BBa_K1491004
BBa_K1491036	.	tag	5063	5080	.	+	0	ID=annotation2380095;Name=6x His;Parent=BBa_K1491004
BBa_K1491036	.	ribosome_entry_site	4328	4339	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1491005
BBa_K1491036	.	conserved_region	4332	4335	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1491036	.	CDS	62	4319	.	+	0	ID=BBa_K1491027;Name=BBa_K1491027
BBa_K1491036	.	CDS	1543	3117	.	+	0	ID=BBa_K1491026;Name=BBa_K1491026;Parent=BBa_K1491027
BBa_K1491036	.	sequence_feature	2916	3117	.	+	0	ID=annotation2384010;Name=C term Intein;Parent=BBa_K1491026
BBa_K1491036	.	non_covalent_binding_site	2158	2915	.	+	0	ID=annotation2384009;Name=Estrogen Receptor;Parent=BBa_K1491026
BBa_K1491036	.	sequence_alteration	3115	3117	.	+	0	ID=annotation2384011;Name=Dead Mutation;Parent=BBa_K1491026
BBa_K1491036	.	sequence_feature	1543	2157	.	+	0	ID=annotation2384008;Name=N term Intein;Parent=BBa_K1491026
BBa_K1491036	.	CDS	62	1534	.	+	0	ID=BBa_K1491022;Name=BBa_K1491022;Parent=BBa_K1491027
BBa_K1491036	.	CDS	3126	4319	.	+	0	ID=BBa_K1491023;Name=BBa_K1491023;Parent=BBa_K1491027
BBa_K1491036	.	tag	4302	4319	.	+	0	ID=annotation2384063;Name=6x His Tag;Parent=BBa_K1491023
BBa_K1491036	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1491036	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1491036	.	promoter	1	35	.	+	0	ID=BBa_J23115;Name=BBa_J23115
>BBa_K1491036
tttatagctagctcagcccttggtacaatgctagctactagagaaagaggagaaatactagatgaacacgattaacatc
gctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttag
ctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaact
taaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatc
aacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccgg
aagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagc
aagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaa
aacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgc
tctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcat
cgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagact
atcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaac
cttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgct
ggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacatt
gcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccgg
tcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcac
cgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatg
cttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacg
ctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaa
ggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaag
ttcattgaggaaaaccacgagaacatcatggcttactagagtgctttgccaagggtaccaatgttttaatggcggatgg
gtctattgaatgtattgagaacattgaggttggtaataaggtcatgggtaaagatggcagacctcgtgaggtaattaaa
ttgcccagaggaagtgaaactatgtacagtgtcgtgcagaaaagtcaacacagagcccataaaagtgactcaagtcgtg
aaatgccagaattactcaagttcacgtgtaatgcgactcatgagttggttgttagaacacctcgtagtgtccgccgttt
gtctcgtaccattaagggtgtcgaatattttgaagttattacttttgagatgggtcaaaagaaggcccccgacggtaga
attgttgagcttgtcaaggaagtttcaaagagctatccagtatccgagggtcctgagagagctaacgaattggtagaat
cctatagaaaggcttcaaataaagcttattttgagtggactattgaggccagggatctttctctgttgggttcccatgt
tcgtaaagctacctaccagacttacgctccaattctttatgagaatgaccactttttcgactacatgcaaaaaagtaag
tttcatctcaccattgaaggtccatctaagaagaacagcctggccttgtccctgacggccgaccagatggtcagtgcct
tgttggatgctgagccccccatactctattccgagtatgatcctaccagacccttcagtgaggcttcgatgatgggctt
actgaccaacctggcagacagggagctggttcacatgatcaactgggcgaagagggtgccaggctttgtggatttgacc
ctccatgatcaggtccaccttctcgaatgtgcctggctagagatcctgatgattggtctcgtctggcgctccatggagc
acccagggaagctactgtttgctcctaacttgctcttggacaggaaccagggaaaatgtgtagagggcatggtggagat
cttcgacatgctgctggctacatcatctcggttccgcatgatgaatctccagggagaggagtttgtgtgcctcaaatct
attattttgcttaattctggagtgtacacatttctgtccagcaccctgaagtctctggaagagaaggaccatatccacc
gagtcctggacaagatcacagacactttgatccacctgatggccaaggcaggcctgaccctccagcagcagcaccagcg
gctggcccagctcctcctcatcctctcccacatcaggcacatgagtaacaaaggcatggagcatctgtacagcatgaag
tgcaagaacgtggtgcccctctatgacctgctgctggagatgctggacgcccaccgcctacatgcgcccaccggtgatg
ttttgcttaacgttctttctaagtgtgccggctctaaaaaattcaggcctgctcccgtcgctacttttgtacgtgagtg
ccagggattttatttcgagttacaagaattaaaggaaaacgattattacggaattaccttatctgatgattctgatcat
cagtttttgcttgccaaccaggttgtcgtccatcagtactagagtgcgctaagtctccactggagaacacttggtgggc
tgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataac
tgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtg
gtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattct
acaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaa
gtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtca
tgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattc
cggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtg
acggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataaga
agactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaa
gaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagat
agcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgta
agactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggc
tgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttc
tacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacc
tccgtgacatcttagagtcggacttcgcgttcgcgcatcatcaccaccatcactactagagaaagaggagaaatactag
atggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcata
agtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaa
gctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatg
aaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggta
actacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaa
agaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacag
aaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagc
aaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaaga
tccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctg
tataaacatcatcaccaccatcactaataa