Constitutive Dead ER Intein T7 RNAP with YFPH
The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the dead ER Intein is BBa_K1491026, the YFP is BBa_K864100
T7 RNA polymerase that contains the dead version estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP. Used as a control.
Constitutive dead ER Intein T7 RNAP with YFP for quantification of T7 RNAP to use as control for WT Intein
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
RBS is BBa_B0034 and SuperYFP2 is BBa_K864100
SuperYFP2 with 6x His tag(BBa_K1491004) and a strong RBS (BBa_B0034). It can be used as a reporter.
T7 RNA Polymerase is BBa_K145001 and dead ER intein is BBa_K1491026
T7 RNA polymerase with a non-functional version of the ER intein. Used as a control.
Control for T7 RNA Polyermase with ER intein (BBa_K1491025)
SuperYFP2 (BBa_K864100) with 6x His tag for protein purification.
Codes for the N-terminus of T7 RNA polymerase (BBa_K145001). This was used to create a T7 polymerase-intein complex.
In order to insert the intein into T7 RNA polymerase, we needed both the N and C terms.
Non functional version of the ER intein (BBa_K1491024) used as a control.
Conversion of the iGEM parts registry to SBOL2.1