BBa_K1491036
type: ComponentDefinition
persistent identity: BBa K1491036
display id: BBa_K1491036
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Dead ER Intein T7 RNAP with YFPH
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the dead ER Intein is BBa_K1491026, the YFP is BBa_K864100
owned by: james
owned by: myers
mutable description: T7 RNA polymerase that contains the dead version estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP. Used as a control.
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constitutive dead ER Intein T7 RNAP with YFP for quantification of T7 RNAP to use as control for WT Intein
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_J23115
component: BBa_K1491005
component: BBa_K1491027
sequence annotation: BBa_J23115
sequence annotation: BBa_B0034
sequence annotation: BBa_K1491027
sequence annotation: BBa_K1491005
sequence: https://synbiohub.org/public/igem/BBa_K1491036_sequence/1

BBa_B0034
type: Component
persistent identity: component2414187
display id: component2414187
version: 1
top level: BBa_K1491036
definition: BBa_B0034
access: public

BBa_J23115
type: Component
persistent identity: component2414185
display id: component2414185
version: 1
top level: BBa_K1491036
definition: BBa_J23115
access: public

BBa_K1491005
type: Component
persistent identity: component2414202
display id: component2414202
version: 1
top level: BBa_K1491036
definition: BBa_K1491005
access: public

BBa_K1491027
type: Component
persistent identity: component2414196
display id: component2414196
version: 1
top level: BBa_K1491036
definition: BBa_K1491027
access: public

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2414185
display id: annotation2414185
version: 1
top level: BBa_K1491036
location: https://synbiohub.org/public/igem/BBa_K1491036/annotation2414185/range2414185/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K1491036/annotation2414185/range2414185/1
type: Range
persistent identity: range2414185
display id: range2414185
version: 1
top level: BBa_K1491036
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2414187
display id: annotation2414187
version: 1
top level: BBa_K1491036
location: https://synbiohub.org/public/igem/BBa_K1491036/annotation2414187/range2414187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491036/annotation2414187/range2414187/1
type: Range
persistent identity: range2414187
display id: range2414187
version: 1
top level: BBa_K1491036
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1491027
type: SequenceAnnotation
persistent identity: annotation2414196
display id: annotation2414196
version: 1
top level: BBa_K1491036
location: https://synbiohub.org/public/igem/BBa_K1491036/annotation2414196/range2414196/1
component: BBa_K1491027

https://synbiohub.org/public/igem/BBa_K1491036/annotation2414196/range2414196/1
type: Range
persistent identity: range2414196
display id: range2414196
version: 1
top level: BBa_K1491036
direction: direction/reverse
start: 62
end: 4319
orientation: inline

BBa_K1491005
type: SequenceAnnotation
persistent identity: annotation2414202
display id: annotation2414202
version: 1
top level: BBa_K1491036
location: https://synbiohub.org/public/igem/BBa_K1491036/annotation2414202/range2414202/1
component: BBa_K1491005

https://synbiohub.org/public/igem/BBa_K1491036/annotation2414202/range2414202/1
type: Range
persistent identity: range2414202
display id: range2414202
version: 1
top level: BBa_K1491036
direction: direction/reverse
start: 4328
end: 5086
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

BBa_K1491005
type: ComponentDefinition
persistent identity: BBa K1491005
display id: BBa_K1491005
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-SuperYFP2-His
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: RBS is BBa_B0034 and SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
mutable description: SuperYFP2 with 6x His tag(BBa_K1491004) and a strong RBS (BBa_B0034). It can be used as a reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: RBS+YFP+His
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1491004
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1491004
sequence: https://synbiohub.org/public/igem/BBa_K1491005_sequence/1

BBa_K1491004
type: Component
persistent identity: component2380100
display id: component2380100
version: 1
top level: BBa_K1491005
definition: BBa_K1491004
access: public

BBa_B0034
type: Component
persistent identity: component2380097
display id: component2380097
version: 1
top level: BBa_K1491005
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380097
display id: annotation2380097
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380097/range2380097/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491005/annotation2380097/range2380097/1
type: Range
persistent identity: range2380097
display id: range2380097
version: 1
top level: BBa_K1491005
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1491004
type: SequenceAnnotation
persistent identity: annotation2380100
display id: annotation2380100
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
component: BBa_K1491004

https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
type: Range
persistent identity: range2380100
display id: range2380100
version: 1
top level: BBa_K1491005
direction: direction/reverse
start: 19
end: 759
orientation: inline

BBa_K1491027
type: ComponentDefinition
persistent identity: BBa K1491027
display id: BBa_K1491027
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA Polymerase with Dead ER Intein
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: T7 RNA Polymerase is BBa_K145001 and dead ER intein is BBa_K1491026
owned by: james
owned by: myers
mutable description: T7 RNA polymerase with a non-functional version of the ER intein. Used as a control.
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Control for T7 RNA Polyermase with ER intein (BBa_K1491025)
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1491023
component: BBa_K1491026
component: BBa_K1491022
sequence annotation: BBa_K1491022
sequence annotation: BBa_K1491026
sequence annotation: BBa_K1491023
sequence: https://synbiohub.org/public/igem/BBa_K1491027_sequence/1

BBa_K1491023
type: Component
persistent identity: component2384107
display id: component2384107
version: 1
top level: BBa_K1491027
definition: BBa_K1491023
access: public

BBa_K1491026
type: Component
persistent identity: component2384105
display id: component2384105
version: 1
top level: BBa_K1491027
definition: BBa_K1491026
access: public

BBa_K1491022
type: Component
persistent identity: component2384100
display id: component2384100
version: 1
top level: BBa_K1491027
definition: BBa_K1491022
access: public

BBa_K1491022
type: SequenceAnnotation
persistent identity: annotation2384100
display id: annotation2384100
version: 1
top level: BBa_K1491027
location: https://synbiohub.org/public/igem/BBa_K1491027/annotation2384100/range2384100/1
component: BBa_K1491022

https://synbiohub.org/public/igem/BBa_K1491027/annotation2384100/range2384100/1
type: Range
persistent identity: range2384100
display id: range2384100
version: 1
top level: BBa_K1491027
direction: direction/reverse
start: 1
end: 1473
orientation: inline

BBa_K1491026
type: SequenceAnnotation
persistent identity: annotation2384105
display id: annotation2384105
version: 1
top level: BBa_K1491027
location: https://synbiohub.org/public/igem/BBa_K1491027/annotation2384105/range2384105/1
component: BBa_K1491026

https://synbiohub.org/public/igem/BBa_K1491027/annotation2384105/range2384105/1
type: Range
persistent identity: range2384105
display id: range2384105
version: 1
top level: BBa_K1491027
direction: direction/reverse
start: 1482
end: 3056
orientation: inline

BBa_K1491023
type: SequenceAnnotation
persistent identity: annotation2384107
display id: annotation2384107
version: 1
top level: BBa_K1491027
location: https://synbiohub.org/public/igem/BBa_K1491027/annotation2384107/range2384107/1
component: BBa_K1491023

https://synbiohub.org/public/igem/BBa_K1491027/annotation2384107/range2384107/1
type: Range
persistent identity: range2384107
display id: range2384107
version: 1
top level: BBa_K1491027
direction: direction/reverse
start: 3065
end: 4258
orientation: inline

BBa_K1491004
type: ComponentDefinition
persistent identity: BBa K1491004
display id: BBa_K1491004
version: 1
was generated by: iGEM to SBOL conversion
description: SuperYFP2-His
created: 2014-07-14T11:00:00Z
modified: 2016-01-21T02:28:40Z
mutable provenance: SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
mutable description: SuperYFP2 (BBa_K864100) with 6x His tag for protein purification.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 4206
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: YFP+His
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K864100
sequence annotation: 6x His
sequence: https://synbiohub.org/public/igem/BBa_K1491004_sequence/1

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2380094
display id: annotation2380094
version: 1
top level: BBa_K1491004
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1
type: Range
persistent identity: range2380094
display id: range2380094
version: 1
top level: BBa_K1491004
direction: direction/forward
start: 1
end: 717
orientation: inline

6x His
type: SequenceAnnotation
persistent identity: annotation2380095
display id: annotation2380095
version: 1
top level: BBa_K1491004
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1
type: Range
persistent identity: range2380095
display id: range2380095
version: 1
top level: BBa_K1491004
direction: direction/forward
start: 718
end: 735
orientation: inline

BBa_K1491022
type: ComponentDefinition
persistent identity: BBa K1491022
display id: BBa_K1491022
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminus of T7 RNA Polymerase
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: BBa_K145001
owned by: james
owned by: myers
mutable description: Codes for the N-terminus of T7 RNA polymerase (BBa_K145001). This was used to create a T7 polymerase-intein complex.
discontinued: false
dominant: false
experience: experience/None
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to insert the intein into T7 RNA polymerase, we needed both the N and C terms.
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1491022_sequence/1

BBa_K1491023
type: ComponentDefinition
persistent identity: BBa K1491023
display id: BBa_K1491023
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminus of T7 RNA Polymerase
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: BBa_K145001
owned by: james
owned by: myers
mutable description: Codes for the N-terminus of T7 RNA polymerase (BBa_K145001). This was used to create a T7 polymerase-intein complex.
discontinued: false
dominant: false
experience: experience/None
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to insert the intein into T7 RNA polymerase, we needed both the N and C terms.
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1491023_sequence/1

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2384063
display id: annotation2384063
version: 1
top level: BBa_K1491023
location: https://synbiohub.org/public/igem/BBa_K1491023/annotation2384063/range2384063/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1491023/annotation2384063/range2384063/1
type: Range
persistent identity: range2384063
display id: range2384063
version: 1
top level: BBa_K1491023
direction: direction/forward
start: 1177
end: 1194
orientation: inline

BBa_K1491026
type: ComponentDefinition
persistent identity: BBa K1491026
display id: BBa_K1491026
version: 1
was generated by: iGEM to SBOL conversion
description: Dead ER Intein
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: Mutations to ER intein (BBa_K1491024)
owned by: james
owned by: myers
mutable description: Non functional version of the ER intein (BBa_K1491024) used as a control.
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Control for ER intein
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N term Intein
sequence annotation: Estrogen Receptor
sequence annotation: C term Intein
sequence annotation: Dead Mutation
sequence: https://synbiohub.org/public/igem/BBa_K1491026_sequence/1

N term Intein
type: SequenceAnnotation
persistent identity: annotation2384008
display id: annotation2384008
version: 1
top level: BBa_K1491026
location: https://synbiohub.org/public/igem/BBa_K1491026/annotation2384008/range2384008/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1491026/annotation2384008/range2384008/1
type: Range
persistent identity: range2384008
display id: range2384008
version: 1
top level: BBa_K1491026
direction: direction/forward
start: 1
end: 615
orientation: inline

Estrogen Receptor
type: SequenceAnnotation
persistent identity: annotation2384009
display id: annotation2384009
version: 1
top level: BBa_K1491026
location: https://synbiohub.org/public/igem/BBa_K1491026/annotation2384009/range2384009/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1491026/annotation2384009/range2384009/1
type: Range
persistent identity: range2384009
display id: range2384009
version: 1
top level: BBa_K1491026
direction: direction/forward
start: 616
end: 1373
orientation: inline

C term Intein
type: SequenceAnnotation
persistent identity: annotation2384010
display id: annotation2384010
version: 1
top level: BBa_K1491026
location: https://synbiohub.org/public/igem/BBa_K1491026/annotation2384010/range2384010/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1491026/annotation2384010/range2384010/1
type: Range
persistent identity: range2384010
display id: range2384010
version: 1
top level: BBa_K1491026
direction: direction/forward
start: 1374
end: 1575
orientation: inline

Dead Mutation
type: SequenceAnnotation
persistent identity: annotation2384011
display id: annotation2384011
version: 1
top level: BBa_K1491026
location: https://synbiohub.org/public/igem/BBa_K1491026/annotation2384011/range2384011/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1491026/annotation2384011/range2384011/1
type: Range
persistent identity: range2384011
display id: range2384011
version: 1
top level: BBa_K1491026
direction: direction/forward
start: 1573
end: 1575
orientation: inline

https://synbiohub.org/public/igem/BBa_K1491036_sequence/1
type: Sequence
persistent identity: BBa K1491036 sequence
display id: BBa_K1491036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatagctagctcagcccttggtacaatgctagctactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttactagagtgctttgccaagggtaccaatgttttaatggcggatgggtctattgaatgtattgagaacattgaggttggtaataaggtcatgggtaaagatggcagacctcgtgaggtaattaaattgcccagaggaagtgaaactatgtacagtgtcgtgcagaaaagtcaacacagagcccataaaagtgactcaagtcgtgaaatgccagaattactcaagttcacgtgtaatgcgactcatgagttggttgttagaacacctcgtagtgtccgccgtttgtctcgtaccattaagggtgtcgaatattttgaagttattacttttgagatgggtcaaaagaaggcccccgacggtagaattgttgagcttgtcaaggaagtttcaaagagctatccagtatccgagggtcctgagagagctaacgaattggtagaatcctatagaaaggcttcaaataaagc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23115_sequence/1
type: Sequence
persistent identity: BBa J23115 sequence
display id: BBa_J23115_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatagctagctcagcccttggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491005_sequence/1
type: Sequence
persistent identity: BBa K1491005 sequence
display id: BBa_K1491005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491027_sequence/1
type: Sequence
persistent identity: BBa K1491027 sequence
display id: BBa_K1491027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttactagagtgctttgccaagggtaccaatgttttaatggcggatgggtctattgaatgtattgagaacattgaggttggtaataaggtcatgggtaaagatggcagacctcgtgaggtaattaaattgcccagaggaagtgaaactatgtacagtgtcgtgcagaaaagtcaacacagagcccataaaagtgactcaagtcgtgaaatgccagaattactcaagttcacgtgtaatgcgactcatgagttggttgttagaacacctcgtagtgtccgccgtttgtctcgtaccattaagggtgtcgaatattttgaagttattacttttgagatgggtcaaaagaaggcccccgacggtagaattgttgagcttgtcaaggaagtttcaaagagctatccagtatccgagggtcctgagagagctaacgaattggtagaatcctatagaaaggcttcaaataaagcttattttgagtggactattgaggccagggatctttctctgttgggttcccatgttcgtaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491004_sequence/1
type: Sequence
persistent identity: BBa K1491004 sequence
display id: BBa_K1491004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491022_sequence/1
type: Sequence
persistent identity: BBa K1491022 sequence
display id: BBa_K1491022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491023_sequence/1
type: Sequence
persistent identity: BBa K1491023 sequence
display id: BBa_K1491023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgcatcatcaccaccatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491026_sequence/1
type: Sequence
persistent identity: BBa K1491026 sequence
display id: BBa_K1491026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgctttgccaagggtaccaatgttttaatggcggatgggtctattgaatgtattgagaacattgaggttggtaataaggtcatgggtaaagatggcagacctcgtgaggtaattaaattgcccagaggaagtgaaactatgtacagtgtcgtgcagaaaagtcaacacagagcccataaaagtgactcaagtcgtgaaatgccagaattactcaagttcacgtgtaatgcgactcatgagttggttgttagaacacctcgtagtgtccgccgtttgtctcgtaccattaagggtgtcgaatattttgaagttattacttttgagatgggtcaaaagaaggcccccgacggtagaattgttgagcttgtcaaggaagtttcaaagagctatccagtatccgagggtcctgagagagctaacgaattggtagaatcctatagaaaggcttcaaataaagcttattttgagtggactattgaggccagggatctttctctgttgggttcccatgttcgtaaagctacctaccagacttacgctccaattctttatgagaatgaccactttttcgactacatgcaaaaaagtaagtttcatctcaccattgaaggtccatctaagaagaacagcctggccttgtccctgacggccgaccagatggtcagtgccttgttggatgctgagccccccatactctattccgagtatgatcctaccagacccttcagtgaggcttcgatgatgggcttactgaccaacctggcagacagggagctggttcacatgatcaactgggcgaagagggtgccaggctttgtggatttgaccctccatgatcaggtccaccttctcgaatgtgcctggctagagatcctgatgattggtctcgtctggcgctccatggagcacccagggaagctactgtttgctcctaacttgctcttggacaggaaccagggaaaatgtgtagagggcatggtggagatcttcgacatgctgctggctacatcatctcggttccgcatgatgaatctccagggagaggagtttgtgtgcctcaaatctattattttgcttaattctggagtgtacacatttctgtccagcaccctgaagtctctggaagagaaggaccatatccaccgagtcctggacaagatcacagacactttgatccacctgatggccaaggcaggcctgaccctccagcagcagcaccagcggctggcccagctcctcctcatcctctcccacatcaggcacatgagtaacaaaggcatggagcatctgtacagcatgaagtgcaagaacgtggtgcccctctatgacctgctgctggagatgctggacgcccaccgcctacatgcgcccaccggtgatgttttgcttaacgttctttctaagtgtgccggctctaaaaaattcaggcctgctcccgtcgctacttttgtacgtgagtgccagggattttatttcgagttacaagaattaaaggaaaacgattattacggaattaccttatctgatgattctgatcatcagtttttgcttgccaaccaggttgtcgtccatcag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z