BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I13504 1 BBa_I13504 Screening plasmid intermediate 2005-05-30T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 Built by Josh as an intermediate in screening plasmid construction. false true _11_ 0 253 6 In stock false true jkm component1505067 1 BBa_B0034 component1505074 1 BBa_B0010 component1505084 1 BBa_B0012 component1505069 1 BBa_E0040 annotation1505069 1 BBa_E0040 range1505069 1 19 738 annotation1505067 1 BBa_B0034 range1505067 1 1 12 annotation1505084 1 BBa_B0012 range1505084 1 835 875 annotation1505074 1 BBa_B0010 range1505074 1 747 826 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1493002 1 BBa_K1493002 Fusaric acid induced promoter 2014-10-08T11:00:00Z 2015-05-08T01:10:45Z - - false false _1873_ 0 21281 9 It's complicated false - false Jeremy van Baalen annotation2428731 1 Promoter Regulator range2428731 1 1 951 annotation2428732 1 Inducible promoter range2428732 1 1035 1117 BBa_K1493003 1 BBa_K1493003 Fusaric acid induced promoter + GFP 2014-10-08T11:00:00Z 2015-05-08T01:10:45Z - - false false _1873_ 0 21281 9 It's complicated false - false Michiel Herpers component2407539 1 BBa_K1493002 component2407549 1 BBa_I13504 annotation2407549 1 BBa_I13504 range2407549 1 1126 2000 annotation2407539 1 BBa_K1493002 range2407539 1 1 1117 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1493003_sequence 1 gttattagttggcgatcagcacctgatgggttttcaggtctgcctcatgcgcggcaagaatctctggcagcgagttgcgcaggtactcgacccaggtcttgatcttggcatccagatactgccgcgatggatagatggcatacaggttcaactcctgcaggcgatactccggcagtacccgtaccaggctaccgtcccgcaggccctcgatagccgaatagatcggcagcacgcctacgcccatgccactgcggatcgcagtcttcatggcatcggcggtgttcaccatgaacggcgaactggtgatgttgaccatctcttggccttccgggccatcgaacagccacttctccagcggtatcaccgggctgaccatgcgcaggcaggcgtgcttgagcaggtcggcaggcttgtgcgccacgccatgctgggcgatgtaggcgggcgaggcacagacaatgctgtaggtgatgcccaggcgctgcgaaacgaaccccgagtcgggcagttcggtggccagcacgatggagacgtcataaccctcgtcgagcagatcgggcacgcggttggccatggtcaaatcgaaggtcacgtccgggtgcgtttcgcggtagcgggcgatggcatcgaccacgaagtgctggccgaccccagtcatcgaatgcaccttcaactgcccggccgggcgcgcatgggcgtcgctggcttcggcctcggcttcttcgacgtaggtaagaatctgttcgcaacgcatcaggtaacgcttgccggcttcggtcagcgcaatgcggcgggtggtacggttgagcagccttgtttgcagatgggcttccaggttggagaccgcccgcgacacgttcgcggtggtcgtatccagttgcgccgcagcggcagtgaagctgccgagttgggctacgcaactgaaagcacgcatgttttgcagggtgtccatgggtcactctcgaggtagaggacaaaattgtgtcacgaagtaacttggaaaagagaaaaagttgccttcgaccaaaggcggattatcgctgttttggtaacaaagattcgcaggaatctgtgcttatcgccagtgctgctgccccctagaattgcccggcccctttactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1493002_sequence 1 gttattagttggcgatcagcacctgatgggttttcaggtctgcctcatgcgcggcaagaatctctggcagcgagttgcgcaggtactcgacccaggtcttgatcttggcatccagatactgccgcgatggatagatggcatacaggttcaactcctgcaggcgatactccggcagtacccgtaccaggctaccgtcccgcaggccctcgatagccgaatagatcggcagcacgcctacgcccatgccactgcggatcgcagtcttcatggcatcggcggtgttcaccatgaacggcgaactggtgatgttgaccatctcttggccttccgggccatcgaacagccacttctccagcggtatcaccgggctgaccatgcgcaggcaggcgtgcttgagcaggtcggcaggcttgtgcgccacgccatgctgggcgatgtaggcgggcgaggcacagacaatgctgtaggtgatgcccaggcgctgcgaaacgaaccccgagtcgggcagttcggtggccagcacgatggagacgtcataaccctcgtcgagcagatcgggcacgcggttggccatggtcaaatcgaaggtcacgtccgggtgcgtttcgcggtagcgggcgatggcatcgaccacgaagtgctggccgaccccagtcatcgaatgcaccttcaactgcccggccgggcgcgcatgggcgtcgctggcttcggcctcggcttcttcgacgtaggtaagaatctgttcgcaacgcatcaggtaacgcttgccggcttcggtcagcgcaatgcggcgggtggtacggttgagcagccttgtttgcagatgggcttccaggttggagaccgcccgcgacacgttcgcggtggtcgtatccagttgcgccgcagcggcagtgaagctgccgagttgggctacgcaactgaaagcacgcatgttttgcagggtgtccatgggtcactctcgaggtagaggacaaaattgtgtcacgaagtaacttggaaaagagaaaaagttgccttcgaccaaaggcggattatcgctgttttggtaacaaagattcgcaggaatctgtgcttatcgccagtgctgctgccccctagaattgcccggcccctt BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_I13504_sequence 1 aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z