LOCUS       BBa_K1493530             970 bp    DNA     linear       22-Nov-2024
DEFINITION  pRHA+TetR
ACCESSION   BBa_K1493530
VERSION     BBa_K1493530.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=pRha
     promoter        1..122
                     /label=BBa_K914003
     misc_feature    135..138
                     /label=conserved
     RBS             131..142
                     /label=BBa_B0034
     CDS             152..768
                     /label=tetR
     misc_feature    769..802
                     /label=SsrA
     misc_feature    809..833
                     /label=Help:Barcodes
     CDS             149..808
                     /label=BBa_C0040
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    842..921
                     /label=BBa_B0010
     terminator      842..921
                     /label=BBa_B0010
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    963..963
                     /label=stop
     polyA_site      957..970
                     /label=polya
     misc_feature    930..970
                     /label=BBa_B0012
     terminator      930..970
                     /label=BBa_B0012
     misc_feature    131..970
                     /label=BBa_P0440
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagag aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa
      181 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
      241 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
      301 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
      361 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
      421 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
      481 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
      541 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
      601 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
      661 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
      721 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga
      781 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata
//