LOCUS       BBa_K1493550            1771 bp    DNA     linear       25-Nov-2024
DEFINITION  pRHA+cIλ+TetR
ACCESSION   BBa_K1493550
VERSION     BBa_K1493550.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=pRha
     promoter        1..122
                     /label=BBa_K914003
     misc_feature    135..138
                     /label=conserved
     RBS             131..142
                     /label=BBa_B0034
     CDS             152..859
                     /label=cI lambda
     misc_feature    860..892
                     /label=LVA
     misc_feature    899..923
                     /label=Help:Barcodes
     CDS             149..898
                     /label=BBa_C0051
     misc_feature    131..923
                     /label=BBa_S0103
     misc_feature    936..939
                     /label=conserved
     RBS             932..943
                     /label=BBa_B0034
     CDS             953..1569
                     /label=tetR
     misc_feature    1570..1603
                     /label=SsrA
     misc_feature    1610..1634
                     /label=Help:Barcodes
     CDS             950..1609
                     /label=BBa_C0040
     stem_loop       1654..1697
                     /label=stem_loop
     misc_feature    1643..1722
                     /label=BBa_B0010
     terminator      1643..1722
                     /label=BBa_B0010
     stem_loop       1738..1757
                     /label=T7 TE
     misc_feature    1764..1764
                     /label=stop
     polyA_site      1758..1771
                     /label=polya
     misc_feature    1731..1771
                     /label=BBa_B0012
     terminator      1731..1771
                     /label=BBa_B0012
     misc_feature    932..1771
                     /label=BBa_P0440
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga
      181 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg
      241 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt
      301 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa
      361 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc
      421 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca
      481 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt
      541 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat
      601 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc
      661 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac
      721 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta
      781 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg
      841 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg
      901 ctgatagtgc tagtgtagat cgctactaga gaaagaggag aaatactaga tgtccagatt
      961 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
     1021 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
     1081 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
     1141 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
     1201 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
     1261 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
     1321 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
     1381 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
     1441 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
     1501 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga
     1561 aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg
     1621 ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1681 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1741 tcaccttcgg gtgggccttt ctgcgtttat a
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