LOCUS       BBa_K1493560            2024 bp    DNA     linear       18-May-2024
DEFINITION  pRHA+cIλ+pcI/tet+GFP
ACCESSION   BBa_K1493560
VERSION     BBa_K1493560.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=pRha
     promoter        1..122
                     /label=BBa_K914003
     misc_feature    135..138
                     /label=conserved
     RBS             131..142
                     /label=BBa_B0034
     CDS             152..859
                     /label=cI lambda
     misc_feature    860..892
                     /label=LVA
     misc_feature    899..923
                     /label=Help:Barcodes
     CDS             149..898
                     /label=BBa_C0051
     stem_loop       943..986
                     /label=stem_loop
     misc_feature    932..1011
                     /label=BBa_B0010
     terminator      932..1011
                     /label=BBa_B0010
     stem_loop       1027..1046
                     /label=T7 TE
     misc_feature    1053..1053
                     /label=stop
     polyA_site      1047..1060
                     /label=polya
     misc_feature    1020..1060
                     /label=BBa_B0012
     terminator      1020..1060
                     /label=BBa_B0012
     misc_feature    131..1060
                     /label=BBa_P0451
     misc_feature    1074..1090
                     /label=cI OR2
     promoter        1088..1093
                     /label=PR -35
     misc_feature    1098..1114
                     /label=cI OR1
     promoter        1111..1116
                     /label=PR -10
     misc_feature    1119..1138
                     /label=TetO1
     promoter        1069..1141
                     /label=BBa_K909013
     misc_feature    1154..1157
                     /label=conserved
     RBS             1150..1161
                     /label=BBa_B0034
     CDS             1168..1887
                     /label=GFP protein
     CDS             1168..1887
                     /label=BBa_E0040
     stem_loop       1907..1950
                     /label=stem_loop
     misc_feature    1896..1975
                     /label=BBa_B0010
     terminator      1896..1975
                     /label=BBa_B0010
     stem_loop       1991..2010
                     /label=T7 TE
     misc_feature    2017..2017
                     /label=stop
     polyA_site      2011..2024
                     /label=polya
     misc_feature    1984..2024
                     /label=BBa_B0012
     terminator      1984..2024
                     /label=BBa_B0012
     misc_feature    1150..2024
                     /label=BBa_I13504
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga
      181 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg
      241 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt
      301 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa
      361 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc
      421 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca
      481 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt
      541 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat
      601 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc
      661 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac
      721 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta
      781 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg
      841 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg
      901 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1021 cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggc ggctaacacc
     1081 gtgcgtgttg actattttac ctctggcggt gataattaat ccctatcagt gatagagatc
     1141 gtactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
     1201 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     1261 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     1321 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     1381 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     1441 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     1501 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     1561 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     1621 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     1681 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     1741 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     1801 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     1861 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
     1921 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1981 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//