LOCUS       BBa_K1493701            1802 bp    DNA     linear       22-Nov-2024
DEFINITION  <i>pCI/Lac</i> + TetR + GFP
ACCESSION   BBa_K1493701
VERSION     BBa_K1493701.1  
FEATURES             Location/Qualifiers
     promoter        20..25
                     /label=PR -35
     promoter        43..48
                     /label=PR -10
     misc_feature    6..22
                     /label=cI OR2
     misc_feature    30..46
                     /label=cI OR1
     misc_feature    50..68
                     /label=LacO1
     promoter        1..71
                     /label=BBa_K909012
     misc_feature    84..87
                     /label=conserved
     RBS             80..91
                     /label=BBa_B0034
     CDS             101..717
                     /label=tetR
     misc_feature    718..751
                     /label=SsrA
     misc_feature    758..782
                     /label=Help:Barcodes
     CDS             98..757
                     /label=BBa_C0040
     stem_loop       802..845
                     /label=stem_loop
     misc_feature    791..870
                     /label=BBa_B0010
     terminator      791..870
                     /label=BBa_B0010
     stem_loop       886..905
                     /label=T7 TE
     misc_feature    912..912
                     /label=stop
     polyA_site      906..919
                     /label=polya
     misc_feature    879..919
                     /label=BBa_B0012
     terminator      879..919
                     /label=BBa_B0012
     misc_feature    80..919
                     /label=BBa_P0440
     misc_feature    932..935
                     /label=conserved
     RBS             928..939
                     /label=BBa_B0034
     CDS             946..1665
                     /label=GFP protein
     CDS             946..1665
                     /label=BBa_E0040
     stem_loop       1685..1728
                     /label=stem_loop
     misc_feature    1674..1753
                     /label=BBa_B0010
     terminator      1674..1753
                     /label=BBa_B0010
     stem_loop       1769..1788
                     /label=T7 TE
     misc_feature    1795..1795
                     /label=stop
     polyA_site      1789..1802
                     /label=polya
     misc_feature    1762..1802
                     /label=BBa_B0012
     terminator      1762..1802
                     /label=BBa_B0012
     misc_feature    928..1802
                     /label=BBa_I13504
ORIGIN
        1 gcggctaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatta attgtgagcg
       61 ctcacaattc gtactagaga aagaggagaa atactagatg tccagattag ataaaagtaa
      121 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
      181 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
      241 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
      301 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
      361 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
      421 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
      481 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
      541 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
      601 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
      661 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
      721 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
      781 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttatat actagagaaa gaggagaaat actagatgcg taaaggagaa
      961 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     1021 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1081 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
     1141 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
     1201 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1261 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     1321 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     1381 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
     1441 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1501 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1561 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     1621 gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca
     1681 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1741 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1801 ta
//