LOCUS       BBa_K1493702            2116 bp    DNA     linear       17-May-2024
DEFINITION  Toggle switch part with reporter gene <i>gfp</i>
ACCESSION   BBa_K1493702
VERSION     BBa_K1493702.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     misc_feature    63..1233
                     /label=BBa_S0100
     misc_feature    1246..1249
                     /label=conserved
     RBS             1242..1253
                     /label=BBa_B0034
     CDS             1260..1979
                     /label=GFP protein
     CDS             1260..1979
                     /label=BBa_E0040
     stem_loop       1999..2042
                     /label=stem_loop
     misc_feature    1988..2067
                     /label=BBa_B0010
     terminator      1988..2067
                     /label=BBa_B0010
     stem_loop       2083..2102
                     /label=T7 TE
     misc_feature    2109..2109
                     /label=stop
     polyA_site      2103..2116
                     /label=polya
     misc_feature    2076..2116
                     /label=BBa_B0012
     terminator      2076..2116
                     /label=BBa_B0012
     misc_feature    1242..2116
                     /label=BBa_I13504
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag aaatactaga
     1261 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1321 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     1381 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     1441 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
     1501 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1561 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1621 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1681 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1741 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1801 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1861 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1921 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1981 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2041 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2101 cctttctgcg tttata
//