BBa_K1497000 1 BBa_K1497000 Chalcone Isomerase (CHI) from Petunia 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z Petunia Hybrida The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. We used the CHI and verified the function in our Naringenin Operon (K1497007). false false _1877_ 0 12466 9 It's complicated false Original Sequence from Petunia false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen BBa_K1497000_sequence 1 atggctcctccagtgtccgttactaaaatgcaggttgagaattacgctttcgcaccgaccgtgaaccctgctggttccaccaataccttgttccttgctggtgctgggcatagaggtctggagatagaagggaagtttgttaagtttacggcgataggtgtgtatctcgaagagagtgctattccttttctggccgaaaaatggaaaggcaaaaccccccaggagttgactgactcggtcgagttctttagggatgttgttacaggtccatttgagaaatttactcgagttactatgatcttgcccttgacgggcaagcagtactcggagaaggtggcggagaattgtgttgcgcattggaaggggataggaacgtatactgatgatgagggtcgtgccattgagaagtttctcgatgttttccggagtgaaacttttccacctggtgcttccatcatgtttactcaatcacccctagggttgttgacgattagcttcgctaaagatgattcagtaactggcactgcgaatgctgttatagagaacaagcagttgtctgaagcagtgctggaatcaataattgggaagcatggagtttctcctgcggcaaagtgtagtgtcgctgaaagagtagcggaactgctcaaaaagagctatgctgaagaggcatctgtttttggaaaaccggagaccgagaaatctactattccagtgattggagtctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z