BBa_K1033000 1 TAL Tyrosine ammonia-lyase (TAL) with RBS 2013-08-14T11:00:00Z 2015-05-08T01:08:48Z Rhodobacter sphaeroides tyrosine ammonia-lyase (TAL) is an enzyme which catalyzes the formation of p-coumaric acid (aka p-hyroxycinnamic acid) from tyrosine. It belongs to the family of ammonia-lyases, enzymes that catalyze the deamination of amino acids [1]. P-coumaric acid is an important precursor in many metabolic pathways. TAL also has a secondary function as a phenylalanine ammonia-lyase (PAL) which catalyses the formation of cinnamic acid. The wild type RsTAL has a preference for tyrosine, however the mutations Met4 -> Ile, Ile325 -> Val and Val409 -> Met, can shift this preference to phenylalanine [2]. 1.Zhixiong Xue, Michael McCluskey, Keith Cantera, F. Sima Sariaslani, Lixuan Huang (2007) Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides. J Ind Microbiol Biotechnol 34:599-604 2. J.A. Kyndt, T.E. Meyer, M.A Cusanovich, J.J. Van Beeumen (2002) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein. FEBS Letters 512 240-244 false false _1340_ 0 18317 9 In stock true Earlier in iGEM eucaryot TAL has been used, however we used bacterial TAL for better compatibility with E.coli. false Karl Holdar, Hampus Elofsson, Emil Marklund, Kristoffer Lundmark, Marcus Hong, Lovisa Pettersson, Ken Braech-Andersen, Theodor L??we annotation2366524 1 B0034 range2366524 1 4 13 annotation2366489 1 Tyrosine amonia lyase range2366489 1 22 1596 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1033001 1 BBa_K1033001 4-coumarate ligase (4CL) with RBS 2013-08-22T11:00:00Z 2015-05-08T01:08:48Z Arabidopsis Thaliana 4-coumarate ligase (4CL) is an enzyme that catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A (4-coumaryl-CoA). false false _1340_ 0 18317 9 It's complicated false Yes. false Karl Holdar annotation2368408 1 4-coumarate-ligase range2368408 1 23 1701 annotation2368409 1 B0034 range2368409 1 4 11 BBa_K1497006 1 BBa_K1497006 B0034-4CL-B0034-TAL 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z w This composite part is the fusion device of the Biobrick parts K1033001 by Uppsala 2013 and K1033000 by. It is able to produce 4-coumaroyl-CoA from tyrosine, if put under a promotor. This device is the first composite part for the assembly of our Naringenin Operon (K1497007). We used the previously announced Biobricks to assemble this part and verified the function in our Naringenin Operon (K1497007). false false _1877_ 0 12466 9 It's complicated false no false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen component2398658 1 BBa_K1033001 component2398663 1 BBa_K1033000 component2398660 1 BBa_B0034 component2398655 1 BBa_B0034 annotation2398655 1 BBa_B0034 range2398655 1 1 12 annotation2398658 1 BBa_K1033001 range2398658 1 21 1728 annotation2398660 1 BBa_B0034 range2398660 1 1737 1748 annotation2398663 1 BBa_K1033000 range2398663 1 1757 3352 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1033000_sequence 1 gtaaaagaggagaaagtagctatgctcgccatgagccccccgaagccggccgtcgagctggatcgccacatcgatctggaccaggcccatgccgtggcgagcggcggcgcgcggattgtccttgcccctccggcgcgcgaccggtgccgtgcgtccgaagcgcggctcggcgctgtcatccgcgaggcgcgccatgtctacggactgacaaccggcttcggtccccttgcgaaccgcctgatctcaggtgagaatgtccgaacgctacaggccaatcttgtccatcatctggccagcggcgtgggaccggtgcttgactggacgacggcgcgcgccatggttctggcgcgtctggtgtcgatcgctcagggagcctccggtgccagcgaggggaccatcgctcgcctgatcgacctgctcaattccgagctcgctccggccgttcccagccgcggcacggtgggcgcgtcgggtgacctgacaccgcttgcgcatatggtgctctgcctccagggccggggagacttcctggaccgggacgggacgcggcttgacggcgcagaagggctccggcgcggacggctgcaaccgctcgatctctcccatcgcgatgcactggcgctggtcaacgggacctccgccatgaccgggatcgcgctggtgaatgctcacgcctgccgccatctcggcaactgggcggtggcgttgacggccctgcttgcggaatgtctgagaggccggaccgaggcatgggccgcggcactgtccgacctgcggccccatcccggacagaaggacgccgcagcgaggctgcgcgcccgcgtggacggcagcgcgcgggtggtccggcacgtcattgccgagcggaggctcgacgccggcgatatcgggacggagccggaggcggggcaggatgcctacagcctgcgctgcgctccgcaggttctcggggcgggcttcgacacgctcgcatggcatgaccgggtgctgacgatcgagctgaacgcggtgaccgacaatccggtgtttccgcccgatggcagcgtgcccgccctgcacgggggcaatttcatgggccagcatgtggcgctgacgtccgatgcgctcgccacggccgtcaccgttctggcgggccttgcggagcgccagattgcacgtctgacagatgaaaggctgaaccgtgggctgccccccttcctccaccggggccccgccgggttgaactccggcttcatgggcgcacaggtgacggcgaccgcgctcctggccgagatgcgagccacgggacctgcctcgatccattcgatctccacgaacgccgccaatcaggatgtggtctcgcttgggaccatcgccgcgcgcctctgccgcgagaagatcgaccgttgggcggagatccttgcgatcctcgctctctgtcttgcacaagctgcggagctgcgctgcggcagcggcctagacggggtgtctcccgcggggaagaagctggtgcaggccctgcgcgagcagttcccgccgcttgagacggaccggcccctgggacaggaaattgccgcgcttgctacgcacctcttgcagcaatctcccgtctgataa BBa_K1497006_sequence 1 aaagaggagaaatactagaggacaaaagaggagaaatctcctatggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgaatttgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgaatttgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaatactagagaaagaggagaaatactagaggtaaaagaggagaaagtagctatgctcgccatgagccccccgaagccggccgtcgagctggatcgccacatcgatctggaccaggcccatgccgtggcgagcggcggcgcgcggattgtccttgcccctccggcgcgcgaccggtgccgtgcgtccgaagcgcggctcggcgctgtcatccgcgaggcgcgccatgtctacggactgacaaccggcttcggtccccttgcgaaccgcctgatctcaggtgagaatgtccgaacgctacaggccaatcttgtccatcatctggccagcggcgtgggaccggtgcttgactggacgacggcgcgcgccatggttctggcgcgtctggtgtcgatcgctcagggagcctccggtgccagcgaggggaccatcgctcgcctgatcgacctgctcaattccgagctcgctccggccgttcccagccgcggcacggtgggcgcgtcgggtgacctgacaccgcttgcgcatatggtgctctgcctccagggccggggagacttcctggaccgggacgggacgcggcttgacggcgcagaagggctccggcgcggacggctgcaaccgctcgatctctcccatcgcgatgcactggcgctggtcaacgggacctccgccatgaccgggatcgcgctggtgaatgctcacgcctgccgccatctcggcaactgggcggtggcgttgacggccctgcttgcggaatgtctgagaggccggaccgaggcatgggccgcggcactgtccgacctgcggccccatcccggacagaaggacgccgcagcgaggctgcgcgcccgcgtggacggcagcgcgcgggtggtccggcacgtcattgccgagcggaggctcgacgccggcgatatcgggacggagccggaggcggggcaggatgcctacagcctgcgctgcgctccgcaggttctcggggcgggcttcgacacgctcgcatggcatgaccgggtgctgacgatcgagctgaacgcggtgaccgacaatccggtgtttccgcccgatggcagcgtgcccgccctgcacgggggcaatttcatgggccagcatgtggcgctgacgtccgatgcgctcgccacggccgtcaccgttctggcgggccttgcggagcgccagattgcacgtctgacagatgaaaggctgaaccgtgggctgccccccttcctccaccggggccccgccgggttgaactccggcttcatgggcgcacaggtgacggcgaccgcgctcctggccgagatgcgagccacgggacctgcctcgatccattcgatctccacgaacgccgccaatcaggatgtggtctcgcttgggaccatcgccgcgcgcctctgccgcgagaagatcgaccgttgggcggagatccttgcgatcctcgctctctgtcttgcacaagctgcggagctgcgctgcggcagcggcctagacggggtgtctcccgcggggaagaagctggtgcaggccctgcgcgagcagttcccgccgcttgagacggaccggcccctgggacaggaaattgccgcgcttgctacgcacctcttgcagcaatctcccgtctgataa BBa_K1033001_sequence 1 gacaaaagaggagaaatctcctatggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgaatttgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgaatttgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z