BBa_K1497007 1 BBa_K1497007 Naringenin producing operon 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z The functionality of this construct is verified by putting the device under a promotor. Proofing functionality leaded to Bba_K1497008 (Naringenin Operon under the constitutive promotor J23100) and Bba_K1497008 (Naringenin Operon under the T7 Promotor). This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes: 4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497100) CHS (Bba_K1497101) For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented. false false _1877_ 0 12466 9 It's complicated true no false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen component2398756 1 BBa_K1033000 component2398758 1 BBa_B0034 component2398753 1 BBa_B0034 component2398751 1 BBa_K801093 component2398762 1 BBa_K1497001 component2398759 1 BBa_K1497000 component2398761 1 BBa_B0034 component2398747 1 BBa_B0034 annotation2398747 1 BBa_B0034 range2398747 1 1 12 annotation2398761 1 BBa_B0034 range2398761 1 4089 4100 annotation2398756 1 BBa_K1033000 range2398756 1 1733 3328 annotation2398751 1 BBa_K801093 range2398751 1 19 1704 annotation2398753 1 BBa_B0034 range2398753 1 1713 1724 annotation2398758 1 BBa_B0034 range2398758 1 3337 3348 annotation2398762 1 BBa_K1497001 range2398762 1 4107 5303 annotation2398759 1 BBa_K1497000 range2398759 1 3355 4080 BBa_K1497001 1 BBa_K1497001 CHS - Chalcone Synthase from Gerbera 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone. We used the CHS and verified the function in our Naringenin Operon (K1497007). The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. false false _1877_ 0 12466 9 It's complicated false no false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen BBa_K1033000 1 TAL Tyrosine ammonia-lyase (TAL) with RBS 2013-08-14T11:00:00Z 2015-05-08T01:08:48Z Rhodobacter sphaeroides tyrosine ammonia-lyase (TAL) is an enzyme which catalyzes the formation of p-coumaric acid (aka p-hyroxycinnamic acid) from tyrosine. It belongs to the family of ammonia-lyases, enzymes that catalyze the deamination of amino acids [1]. P-coumaric acid is an important precursor in many metabolic pathways. TAL also has a secondary function as a phenylalanine ammonia-lyase (PAL) which catalyses the formation of cinnamic acid. The wild type RsTAL has a preference for tyrosine, however the mutations Met4 -> Ile, Ile325 -> Val and Val409 -> Met, can shift this preference to phenylalanine [2]. 1.Zhixiong Xue, Michael McCluskey, Keith Cantera, F. Sima Sariaslani, Lixuan Huang (2007) Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides. J Ind Microbiol Biotechnol 34:599-604 2. J.A. Kyndt, T.E. Meyer, M.A Cusanovich, J.J. Van Beeumen (2002) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein. FEBS Letters 512 240-244 false false _1340_ 0 18317 9 In stock true Earlier in iGEM eucaryot TAL has been used, however we used bacterial TAL for better compatibility with E.coli. false Karl Holdar, Hampus Elofsson, Emil Marklund, Kristoffer Lundmark, Marcus Hong, Lovisa Pettersson, Ken Braech-Andersen, Theodor L??we annotation2366524 1 B0034 range2366524 1 4 13 annotation2366489 1 Tyrosine amonia lyase range2366489 1 22 1596 BBa_K801093 1 BBa_K801093 4-coumarate--coenzyme A ligase (4CL) coding region 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991 Released HQ 2013 4-coumarate--coenzyme A ligase (4CL) coding region from ''Arabidopsis thaliana''. false false _1057_ 0 13150 9 In stock false - false Ingmar Polte annotation2202141 1 Stop codon range2202141 1 1684 1686 annotation2202140 1 Start codon range2202140 1 1 3 annotation2202143 1 4CL range2202143 1 1 1683 BBa_K1497000 1 BBa_K1497000 Chalcone Isomerase (CHI) from Petunia 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z Petunia Hybrida The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. We used the CHI and verified the function in our Naringenin Operon (K1497007). false false _1877_ 0 12466 9 It's complicated false Original Sequence from Petunia false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1497001_sequence 1 atggcgtcctccgttgacatgaaggcgatcagagatgctcaacgtgcagaaggtccggcgaccattcttgccatcggaactgcaactccggcgaattgcgtctatcaagcggattatcccgattactattttcggatcaccaagagtgaacacatggtggatctcaaagagaaattcaagcgcatgtgtgacaagtcgatgataaggaaacgttacatgcacatcacagaggagtatcttaaacaaaaccctaacatgtgcgcgtacatggcgccgtcgctcgacgtccggcaagacctggtcgtcgtcgaagtcccaaagctcggcaaggaagccgccatgaaagccatcaaagaatggggccaccccaaatccaagatcacccacctcatcttctgcaccacctccggcgtcgacatgcccggcgccgactaccagctcaccaaactcctcggtctccggccatccgtcaaacgcttcatgatgtaccaacaaggctgcttcgccggcggcacggttctccggctagccaaagatctcgcggagaacaataaaggcgctagggttcttgtggtgtgctccgagatcacggcggtgactttccgaggacctaatgacacccaccttgattccctagtcggacaggccttgttcggcgacggggctgcggcggtgatcgtgggttccgatcccgacttgacgacggagcggccgttgtttgaaatggtttccgccgctcagacgatcttgccggactccgagggagccattgatggacacttgagggaagtagggttgacgtttcatttactcaaagacgtgcctgggttgatatcgaagaacatagagaaagctttaacgacggcgttttctccgttgggtatcaacgactggaactcgatattctggatagcacatcccggaggtccggcgatactggaccaggtggagctcaagctagggttgaaggaggagaagcttagagctactagacatgttttaagcgagtacggtaacatgtcaagtgcttgtgtgttgtttattatcgacgaaatgagaaagaagtcgtcggagaacggcgccggcaccaccggagaaggtttggagtggggtgttctgtttgggtttgggcctgggttgacggtggagacggtggttcttcacagtgtcccaaccaccgtgacggttcccgtctaa BBa_K801093_sequence 1 atggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgagttcgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgagttcgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaa BBa_K1497000_sequence 1 atggctcctccagtgtccgttactaaaatgcaggttgagaattacgctttcgcaccgaccgtgaaccctgctggttccaccaataccttgttccttgctggtgctgggcatagaggtctggagatagaagggaagtttgttaagtttacggcgataggtgtgtatctcgaagagagtgctattccttttctggccgaaaaatggaaaggcaaaaccccccaggagttgactgactcggtcgagttctttagggatgttgttacaggtccatttgagaaatttactcgagttactatgatcttgcccttgacgggcaagcagtactcggagaaggtggcggagaattgtgttgcgcattggaaggggataggaacgtatactgatgatgagggtcgtgccattgagaagtttctcgatgttttccggagtgaaacttttccacctggtgcttccatcatgtttactcaatcacccctagggttgttgacgattagcttcgctaaagatgattcagtaactggcactgcgaatgctgttatagagaacaagcagttgtctgaagcagtgctggaatcaataattgggaagcatggagtttctcctgcggcaaagtgtagtgtcgctgaaagagtagcggaactgctcaaaaagagctatgctgaagaggcatctgtttttggaaaaccggagaccgagaaatctactattccagtgattggagtctag BBa_B0034_sequence 1 aaagaggagaaa BBa_K1033000_sequence 1 gtaaaagaggagaaagtagctatgctcgccatgagccccccgaagccggccgtcgagctggatcgccacatcgatctggaccaggcccatgccgtggcgagcggcggcgcgcggattgtccttgcccctccggcgcgcgaccggtgccgtgcgtccgaagcgcggctcggcgctgtcatccgcgaggcgcgccatgtctacggactgacaaccggcttcggtccccttgcgaaccgcctgatctcaggtgagaatgtccgaacgctacaggccaatcttgtccatcatctggccagcggcgtgggaccggtgcttgactggacgacggcgcgcgccatggttctggcgcgtctggtgtcgatcgctcagggagcctccggtgccagcgaggggaccatcgctcgcctgatcgacctgctcaattccgagctcgctccggccgttcccagccgcggcacggtgggcgcgtcgggtgacctgacaccgcttgcgcatatggtgctctgcctccagggccggggagacttcctggaccgggacgggacgcggcttgacggcgcagaagggctccggcgcggacggctgcaaccgctcgatctctcccatcgcgatgcactggcgctggtcaacgggacctccgccatgaccgggatcgcgctggtgaatgctcacgcctgccgccatctcggcaactgggcggtggcgttgacggccctgcttgcggaatgtctgagaggccggaccgaggcatgggccgcggcactgtccgacctgcggccccatcccggacagaaggacgccgcagcgaggctgcgcgcccgcgtggacggcagcgcgcgggtggtccggcacgtcattgccgagcggaggctcgacgccggcgatatcgggacggagccggaggcggggcaggatgcctacagcctgcgctgcgctccgcaggttctcggggcgggcttcgacacgctcgcatggcatgaccgggtgctgacgatcgagctgaacgcggtgaccgacaatccggtgtttccgcccgatggcagcgtgcccgccctgcacgggggcaatttcatgggccagcatgtggcgctgacgtccgatgcgctcgccacggccgtcaccgttctggcgggccttgcggagcgccagattgcacgtctgacagatgaaaggctgaaccgtgggctgccccccttcctccaccggggccccgccgggttgaactccggcttcatgggcgcacaggtgacggcgaccgcgctcctggccgagatgcgagccacgggacctgcctcgatccattcgatctccacgaacgccgccaatcaggatgtggtctcgcttgggaccatcgccgcgcgcctctgccgcgagaagatcgaccgttgggcggagatccttgcgatcctcgctctctgtcttgcacaagctgcggagctgcgctgcggcagcggcctagacggggtgtctcccgcggggaagaagctggtgcaggccctgcgcgagcagttcccgccgcttgagacggaccggcccctgggacaggaaattgccgcgcttgctacgcacctcttgcagcaatctcccgtctgataa BBa_K1497007_sequence 1 aaagaggagaaatactagatggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgagttcgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgagttcgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaatactagagaaagaggagaaatactagaggtaaaagaggagaaagtagctatgctcgccatgagccccccgaagccggccgtcgagctggatcgccacatcgatctggaccaggcccatgccgtggcgagcggcggcgcgcggattgtccttgcccctccggcgcgcgaccggtgccgtgcgtccgaagcgcggctcggcgctgtcatccgcgaggcgcgccatgtctacggactgacaaccggcttcggtccccttgcgaaccgcctgatctcaggtgagaatgtccgaacgctacaggccaatcttgtccatcatctggccagcggcgtgggaccggtgcttgactggacgacggcgcgcgccatggttctggcgcgtctggtgtcgatcgctcagggagcctccggtgccagcgaggggaccatcgctcgcctgatcgacctgctcaattccgagctcgctccggccgttcccagccgcggcacggtgggcgcgtcgggtgacctgacaccgcttgcgcatatggtgctctgcctccagggccggggagacttcctggaccgggacgggacgcggcttgacggcgcagaagggctccggcgcggacggctgcaaccgctcgatctctcccatcgcgatgcactggcgctggtcaacgggacctccgccatgaccgggatcgcgctggtgaatgctcacgcctgccgccatctcggcaactgggcggtggcgttgacggccctgcttgcggaatgtctgagaggccggaccgaggcatgggccgcggcactgtccgacctgcggccccatcccggacagaaggacgccgcagcgaggctgcgcgcccgcgtggacggcagcgcgcgggtggtccggcacgtcattgccgagcggaggctcgacgccggcgatatcgggacggagccggaggcggggcaggatgcctacagcctgcgctgcgctccgcaggttctcggggcgggcttcgacacgctcgcatggcatgaccgggtgctgacgatcgagctgaacgcggtgaccgacaatccggtgtttccgcccgatggcagcgtgcccgccctgcacgggggcaatttcatgggccagcatgtggcgctgacgtccgatgcgctcgccacggccgtcaccgttctggcgggccttgcggagcgccagattgcacgtctgacagatgaaaggctgaaccgtgggctgccccccttcctccaccggggccccgccgggttgaactccggcttcatgggcgcacaggtgacggcgaccgcgctcctggccgagatgcgagccacgggacctgcctcgatccattcgatctccacgaacgccgccaatcaggatgtggtctcgcttgggaccatcgccgcgcgcctctgccgcgagaagatcgaccgttgggcggagatccttgcgatcctcgctctctgtcttgcacaagctgcggagctgcgctgcggcagcggcctagacggggtgtctcccgcggggaagaagctggtgcaggccctgcgcgagcagttcccgccgcttgagacggaccggcccctgggacaggaaattgccgcgcttgctacgcacctcttgcagcaatctcccgtctgataatactagagaaagaggagaaatactagatggctcctccagtgtccgttactaaaatgcaggttgagaattacgctttcgcaccgaccgtgaaccctgctggttccaccaataccttgttccttgctggtgctgggcatagaggtctggagatagaagggaagtttgttaagtttacggcgataggtgtgtatctcgaagagagtgctattccttttctggccgaaaaatggaaaggcaaaaccccccaggagttgactgactcggtcgagttctttagggatgttgttacaggtccatttgagaaatttactcgagttactatgatcttgcccttgacgggcaagcagtactcggagaaggtggcggagaattgtgttgcgcattggaaggggataggaacgtatactgatgatgagggtcgtgccattgagaagtttctcgatgttttccggagtgaaacttttccacctggtgcttccatcatgtttactcaatcacccctagggttgttgacgattagcttcgctaaagatgattcagtaactggcactgcgaatgctgttatagagaacaagcagttgtctgaagcagtgctggaatcaataattgggaagcatggagtttctcctgcggcaaagtgtagtgtcgctgaaagagtagcggaactgctcaaaaagagctatgctgaagaggcatctgtttttggaaaaccggagaccgagaaatctactattccagtgattggagtctagtactagagaaagaggagaaatactagatggcgtcctccgttgacatgaaggcgatcagagatgctcaacgtgcagaaggtccggcgaccattcttgccatcggaactgcaactccggcgaattgcgtctatcaagcggattatcccgattactattttcggatcaccaagagtgaacacatggtggatctcaaagagaaattcaagcgcatgtgtgacaagtcgatgataaggaaacgttacatgcacatcacagaggagtatcttaaacaaaaccctaacatgtgcgcgtacatggcgccgtcgctcgacgtccggcaagacctggtcgtcgtcgaagtcccaaagctcggcaaggaagccgccatgaaagccatcaaagaatggggccaccccaaatccaagatcacccacctcatcttctgcaccacctccggcgtcgacatgcccggcgccgactaccagctcaccaaactcctcggtctccggccatccgtcaaacgcttcatgatgtaccaacaaggctgcttcgccggcggcacggttctccggctagccaaagatctcgcggagaacaataaaggcgctagggttcttgtggtgtgctccgagatcacggcggtgactttccgaggacctaatgacacccaccttgattccctagtcggacaggccttgttcggcgacggggctgcggcggtgatcgtgggttccgatcccgacttgacgacggagcggccgttgtttgaaatggtttccgccgctcagacgatcttgccggactccgagggagccattgatggacacttgagggaagtagggttgacgtttcatttactcaaagacgtgcctgggttgatatcgaagaacatagagaaagctttaacgacggcgttttctccgttgggtatcaacgactggaactcgatattctggatagcacatcccggaggtccggcgatactggaccaggtggagctcaagctagggttgaaggaggagaagcttagagctactagacatgttttaagcgagtacggtaacatgtcaagtgcttgtgtgttgtttattatcgacgaaatgagaaagaagtcgtcggagaacggcgccggcaccaccggagaaggtttggagtggggtgttctgtttgggtttgggcctgggttgacggtggagacggtggttcttcacagtgtcccaaccaccgtgacggttcccgtctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z