BBa_K1497016
type: ComponentDefinition
persistent identity: BBa K1497016
display id: BBa_K1497016
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive naringenin producing operon
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
mutable provenance: d
owned by: james
owned by: myers
mutable description: d
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: d
star: false
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1497007
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K1497007
sequence: https://synbiohub.org/public/igem/BBa_K1497016_sequence/1

BBa_K1497007
type: Component
persistent identity: component2398929
display id: component2398929
version: 1
top level: BBa_K1497016
definition: BBa_K1497007
access: public

BBa_J23100
type: Component
persistent identity: component2398911
display id: component2398911
version: 1
top level: BBa_K1497016
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2398911
display id: annotation2398911
version: 1
top level: BBa_K1497016
location: https://synbiohub.org/public/igem/BBa_K1497016/annotation2398911/range2398911/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1497016/annotation2398911/range2398911/1
type: Range
persistent identity: range2398911
display id: range2398911
version: 1
top level: BBa_K1497016
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1497007
type: SequenceAnnotation
persistent identity: annotation2398929
display id: annotation2398929
version: 1
top level: BBa_K1497016
location: https://synbiohub.org/public/igem/BBa_K1497016/annotation2398929/range2398929/1
component: BBa_K1497007

https://synbiohub.org/public/igem/BBa_K1497016/annotation2398929/range2398929/1
type: Range
persistent identity: range2398929
display id: range2398929
version: 1
top level: BBa_K1497016
direction: direction/reverse
start: 44
end: 5346
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K1497007
type: ComponentDefinition
persistent identity: BBa K1497007
display id: BBa_K1497007
version: 1
was generated by: iGEM to SBOL conversion
description: Naringenin producing operon
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
mutable provenance: The functionality of this construct is verified by putting the device under a promotor. Proofing functionality leaded to Bba_K1497008 (Naringenin Operon under the constitutive promotor J23100) and Bba_K1497008 (Naringenin Operon under the T7 Promotor).
owned by: james
owned by: myers
mutable description: This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes: 4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497100) CHS (Bba_K1497101) For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: no
star: false
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497000
component: BBa_B0034
component: BBa_K1033000
component: BBa_K801093
component: BBa_K1497001
sequence annotation: BBa_B0034
sequence annotation: BBa_K801093
sequence annotation: BBa_K1033000
sequence annotation: BBa_K1497000
sequence annotation: BBa_K1497001
sequence: https://synbiohub.org/public/igem/BBa_K1497007_sequence/1

BBa_K1497000
type: Component
persistent identity: component2398759
display id: component2398759
version: 1
top level: BBa_K1497007
definition: BBa_K1497000
access: public

BBa_B0034
type: Component
persistent identity: component2398758
display id: component2398758
version: 1
top level: BBa_K1497007
definition: BBa_B0034
access: public

BBa_K1033000
type: Component
persistent identity: component2398756
display id: component2398756
version: 1
top level: BBa_K1497007
definition: TAL
access: public

BBa_B0034
type: Component
persistent identity: component2398753
display id: component2398753
version: 1
top level: BBa_K1497007
definition: BBa_B0034
access: public

BBa_K801093
type: Component
persistent identity: component2398751
display id: component2398751
version: 1
top level: BBa_K1497007
definition: BBa_K801093
access: public

BBa_B0034
type: Component
persistent identity: component2398747
display id: component2398747
version: 1
top level: BBa_K1497007
definition: BBa_B0034
access: public

BBa_K1497001
type: Component
persistent identity: component2398762
display id: component2398762
version: 1
top level: BBa_K1497007
definition: BBa_K1497001
access: public

BBa_B0034
type: Component
persistent identity: component2398761
display id: component2398761
version: 1
top level: BBa_K1497007
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398747
display id: annotation2398747
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398747/range2398747/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398747/range2398747/1
type: Range
persistent identity: range2398747
display id: range2398747
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K801093
type: SequenceAnnotation
persistent identity: annotation2398751
display id: annotation2398751
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398751/range2398751/1
component: BBa_K801093

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398751/range2398751/1
type: Range
persistent identity: range2398751
display id: range2398751
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 19
end: 1704
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398753
display id: annotation2398753
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398753/range2398753/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398753/range2398753/1
type: Range
persistent identity: range2398753
display id: range2398753
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 1713
end: 1724
orientation: inline

BBa_K1033000
type: SequenceAnnotation
persistent identity: annotation2398756
display id: annotation2398756
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398756/range2398756/1
component: BBa_K1033000

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398756/range2398756/1
type: Range
persistent identity: range2398756
display id: range2398756
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 1733
end: 3328
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398758
display id: annotation2398758
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398758/range2398758/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398758/range2398758/1
type: Range
persistent identity: range2398758
display id: range2398758
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 3337
end: 3348
orientation: inline

BBa_K1497000
type: SequenceAnnotation
persistent identity: annotation2398759
display id: annotation2398759
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398759/range2398759/1
component: BBa_K1497000

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398759/range2398759/1
type: Range
persistent identity: range2398759
display id: range2398759
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 3355
end: 4080
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398761
display id: annotation2398761
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398761/range2398761/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398761/range2398761/1
type: Range
persistent identity: range2398761
display id: range2398761
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 4089
end: 4100
orientation: inline

BBa_K1497001
type: SequenceAnnotation
persistent identity: annotation2398762
display id: annotation2398762
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398762/range2398762/1
component: BBa_K1497001

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398762/range2398762/1
type: Range
persistent identity: range2398762
display id: range2398762
version: 1
top level: BBa_K1497007
direction: direction/reverse
start: 4107
end: 5303
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

TAL
type: ComponentDefinition
persistent identity: BBa K1033000
display id: BBa_K1033000
version: 1
was generated by: iGEM to SBOL conversion
description: Tyrosine ammonia-lyase (TAL) with RBS
created: 2013-08-14T11:00:00Z
modified: 2015-05-08T01:08:48Z
mutable provenance: Rhodobacter sphaeroides
owned by: james
owned by: myers
mutable description: tyrosine ammonia-lyase (TAL) is an enzyme which catalyzes the formation of p-coumaric acid (aka p-hyroxycinnamic acid) from tyrosine. It belongs to the family of ammonia-lyases, enzymes that catalyze the deamination of amino acids [1]. P-coumaric acid is an important precursor in many metabolic pathways. TAL also has a secondary function as a phenylalanine ammonia-lyase (PAL) which catalyses the formation of cinnamic acid. The wild type RsTAL has a preference for tyrosine, however the mutations Met4 -> Ile, Ile325 -> Val and Val409 -> Met, can shift this preference to phenylalanine [2]. 1.Zhixiong Xue, Michael McCluskey, Keith Cantera, F. Sima Sariaslani, Lixuan Huang (2007) Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides. J Ind Microbiol Biotechnol 34:599-604 2. J.A. Kyndt, T.E. Meyer, M.A Cusanovich, J.J. Van Beeumen (2002) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein. FEBS Letters 512 240-244
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 18317
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Earlier in iGEM eucaryot TAL has been used, however we used bacterial TAL for better compatibility with E.coli.
star: false
creator: Karl Holdar, Hampus Elofsson, Emil Marklund, Kristoffer Lundmark, Marcus Hong, Lovisa Pettersson, Ken Braech-Andersen, Theodor L??we
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: Tyrosine amonia lyase
sequence: https://synbiohub.org/public/igem/BBa_K1033000_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2366524
display id: annotation2366524
version: 1
top level: TAL
location: https://synbiohub.org/public/igem/BBa_K1033000/annotation2366524/range2366524/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1033000/annotation2366524/range2366524/1
type: Range
persistent identity: range2366524
display id: range2366524
version: 1
top level: TAL
direction: direction/forward
start: 4
end: 13
orientation: inline

Tyrosine amonia lyase
type: SequenceAnnotation
persistent identity: annotation2366489
display id: annotation2366489
version: 1
top level: TAL
location: https://synbiohub.org/public/igem/BBa_K1033000/annotation2366489/range2366489/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1033000/annotation2366489/range2366489/1
type: Range
persistent identity: range2366489
display id: range2366489
version: 1
top level: TAL
direction: direction/forward
start: 22
end: 1596
orientation: inline

BBa_K801093
type: ComponentDefinition
persistent identity: BBa K801093
display id: BBa_K801093
version: 1
was generated by: iGEM to SBOL conversion
description: 4-coumarate--coenzyme A ligase (4CL) coding region
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 4-coumarate--coenzyme A ligase (4CL) coding region from ''Arabidopsis thaliana''.
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Ingmar Polte
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: 4CL
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801093_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2202140
display id: annotation2202140
version: 1
top level: BBa_K801093
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1
type: Range
persistent identity: range2202140
display id: range2202140
version: 1
top level: BBa_K801093
direction: direction/forward
start: 1
end: 3
orientation: inline

4CL
type: SequenceAnnotation
persistent identity: annotation2202143
display id: annotation2202143
version: 1
top level: BBa_K801093
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1
type: Range
persistent identity: range2202143
display id: range2202143
version: 1
top level: BBa_K801093
direction: direction/forward
start: 1
end: 1683
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202141
display id: annotation2202141
version: 1
top level: BBa_K801093
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1
type: Range
persistent identity: range2202141
display id: range2202141
version: 1
top level: BBa_K801093
direction: direction/forward
start: 1684
end: 1686
orientation: inline

BBa_K1497000
type: ComponentDefinition
persistent identity: BBa K1497000
display id: BBa_K1497000
version: 1
was generated by: iGEM to SBOL conversion
description: Chalcone Isomerase (CHI) from Petunia
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
mutable provenance: Petunia Hybrida
owned by: james
owned by: myers
mutable description: The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. We used the CHI and verified the function in our Naringenin Operon (K1497007).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Original Sequence from Petunia
star: false
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1497000_sequence/1

BBa_K1497001
type: ComponentDefinition
persistent identity: BBa K1497001
display id: BBa_K1497001
version: 1
was generated by: iGEM to SBOL conversion
description: CHS - Chalcone Synthase from Gerbera
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
mutable provenance: Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone. We used the CHS and verified the function in our Naringenin Operon (K1497007).
owned by: james
owned by: myers
mutable description: The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497001_sequence/1

https://synbiohub.org/public/igem/BBa_K1497016_sequence/1
type: Sequence
persistent identity: BBa K1497016 sequence
display id: BBa_K1497016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgagttcgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgagttcgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaatactagagaaagaggagaaatactagaggtaaaagaggagaaagtagctatgctcgccatgagccccccgaagccggccgtcgagctggatcgccacatcgatctggaccaggcccatgccgtggcgagcggcggcgcgcggattgtccttgcccctccggcgcgcgaccggtgccgtgcgtccgaagcgcggctcggcgctgtcatccgcgaggcgcgccatgtctacggactgacaaccggcttcggtccc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1497007_sequence/1
type: Sequence
persistent identity: BBa K1497007 sequence
display id: BBa_K1497007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgagttcgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgagttcgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaatactagagaaagaggagaaatactagaggtaaaagaggagaaagtagctatgctcgccatgagccccccgaagccggccgtcgagctggatcgccacatcgatctggaccaggcccatgccgtggcgagcggcggcgcgcggattgtccttgcccctccggcgcgcgaccggtgccgtgcgtccgaagcgcggctcggcgctgtcatccgcgaggcgcgccatgtctacggactgacaaccggcttcggtccccttgcgaaccgcctgatctcaggtgagaatgtccgaacgctac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1033000_sequence/1
type: Sequence
persistent identity: BBa K1033000 sequence
display id: BBa_K1033000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaaaagaggagaaagtagctatgctcgccatgagccccccgaagccggccgtcgagctggatcgccacatcgatctggaccaggcccatgccgtggcgagcggcggcgcgcggattgtccttgcccctccggcgcgcgaccggtgccgtgcgtccgaagcgcggctcggcgctgtcatccgcgaggcgcgccatgtctacggactgacaaccggcttcggtccccttgcgaaccgcctgatctcaggtgagaatgtccgaacgctacaggccaatcttgtccatcatctggccagcggcgtgggaccggtgcttgactggacgacggcgcgcgccatggttctggcgcgtctggtgtcgatcgctcagggagcctccggtgccagcgaggggaccatcgctcgcctgatcgacctgctcaattccgagctcgctccggccgttcccagccgcggcacggtgggcgcgtcgggtgacctgacaccgcttgcgcatatggtgctctgcctccagggccggggagacttcctggaccgggacgggacgcggcttgacggcgcagaagggctccggcgcggacggctgcaaccgctcgatctctcccatcgcgatgcactggcgctggtcaacgggacctccgccatgaccgggatcgcgctggtgaatgctcacgcctgccgccatctcggcaactgggcggtggcgttgacggccctgcttgcggaatgtctgagaggccggaccgaggcatgggccgcggcactgtccgacctgcggccccatcccggacagaaggacgccgcagcgaggctgcgcgcccgcgtggacggcagcgcgcgggtggtccggcacgtcattgccgagcggaggctcgacgccggcgatatcgggacggagccggaggcggggcaggatgcctacagcctgcgctgcgctccgcaggttctcggggcgggcttcgacacgctcgcatggcatgaccgggtgctgacgatcgagctgaacgcggtgaccgacaatccggtgtttccgcccgatggcagcgtgcccgccctgcacgggggcaatttcatgggccagcatgtggcgctgacgtccgatgcgctcgccacggccgtcaccgttctggcgggccttgcggagcgccagattgcacgtctgacagatgaaaggctgaaccgtgggctgccccccttcctccaccggggccccgccgggttgaactccggcttcatgggcgcacaggtgacggcgaccgcgctcctggccgagatgcgagccacgggacctgcctcgatccattcgatctccacgaacgccgccaatcaggatgtggtctcgcttgggaccatcgccgcgcgcctctgccgcgagaagatcgaccgttgggcggagatccttgcgatcctcgctctctgtcttgcacaagctgcggagctgcgctgcggcagcggcctagacggggtgtctcccgcggggaagaagctggtgcaggccctgcgcgagcagttcccgccgcttgagacggaccggcccctgggacaggaaattgccgcgcttgctacgcacctcttgcagcaatctcccgtctgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801093_sequence/1
type: Sequence
persistent identity: BBa K801093 sequence
display id: BBa_K801093_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgagttcgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgagttcgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1497000_sequence/1
type: Sequence
persistent identity: BBa K1497000 sequence
display id: BBa_K1497000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctcctccagtgtccgttactaaaatgcaggttgagaattacgctttcgcaccgaccgtgaaccctgctggttccaccaataccttgttccttgctggtgctgggcatagaggtctggagatagaagggaagtttgttaagtttacggcgataggtgtgtatctcgaagagagtgctattccttttctggccgaaaaatggaaaggcaaaaccccccaggagttgactgactcggtcgagttctttagggatgttgttacaggtccatttgagaaatttactcgagttactatgatcttgcccttgacgggcaagcagtactcggagaaggtggcggagaattgtgttgcgcattggaaggggataggaacgtatactgatgatgagggtcgtgccattgagaagtttctcgatgttttccggagtgaaacttttccacctggtgcttccatcatgtttactcaatcacccctagggttgttgacgattagcttcgctaaagatgattcagtaactggcactgcgaatgctgttatagagaacaagcagttgtctgaagcagtgctggaatcaataattgggaagcatggagtttctcctgcggcaaagtgtagtgtcgctgaaagagtagcggaactgctcaaaaagagctatgctgaagaggcatctgtttttggaaaaccggagaccgagaaatctactattccagtgattggagtctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1497001_sequence/1
type: Sequence
persistent identity: BBa K1497001 sequence
display id: BBa_K1497001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgtcctccgttgacatgaaggcgatcagagatgctcaacgtgcagaaggtccggcgaccattcttgccatcggaactgcaactccggcgaattgcgtctatcaagcggattatcccgattactattttcggatcaccaagagtgaacacatggtggatctcaaagagaaattcaagcgcatgtgtgacaagtcgatgataaggaaacgttacatgcacatcacagaggagtatcttaaacaaaaccctaacatgtgcgcgtacatggcgccgtcgctcgacgtccggcaagacctggtcgtcgtcgaagtcccaaagctcggcaaggaagccgccatgaaagccatcaaagaatggggccaccccaaatccaagatcacccacctcatcttctgcaccacctccggcgtcgacatgcccggcgccgactaccagctcaccaaactcctcggtctccggccatccgtcaaacgcttcatgatgtaccaacaaggctgcttcgccggcggcacggttctccggctagccaaagatctcgcggagaacaataaaggcgctagggttcttgtggtgtgctccgagatcacggcggtgactttccgaggacctaatgacacccaccttgattccctagtcggacaggccttgttcggcgacggggctgcggcggtgatcgtgggttccgatcccgacttgacgacggagcggccgttgtttgaaatggtttccgccgctcagacgatcttgccggactccgagggagccattgatggacacttgagggaagtagggttgacgtttcatttactcaaagacgtgcctgggttgatatcgaagaacatagagaaagctttaacgacggcgttttctccgttgggtatcaacgactggaactcgatattctggatagcacatcccggaggtccggcgatactggaccaggtggagctcaagctagggttgaaggaggagaagcttagagctactagacatgttttaagcgagtacggtaacatgtcaagtgcttgtgtgttgtttattatcgacgaaatgagaaagaagtcgtcggagaacggcgccggcaccaccggagaaggtttggagtggggtgttctgtttgggtttgggcctgggttgacggtggagacggtggttcttcacagtgtcccaaccaccgtgacggttcccgtctaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z