BBa_K1497019 1 BBa_K1497019 fdeR (Naringenin binding protein) and fde operon regulatory domain 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d fdeR - Naringenin binding protein We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP K1497020 mKate (DsRed variant) K1497021 CFP K1497022 You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device. false false _1877_ 0 12466 9 It's complicated true d false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen BBa_K1497021 1 BBa_K1497021 Naringenin sensor (FdeR) with mKate as reporter 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP fluorescence K1497020 mKate (DsRed variant) fluorescence K1497021 CFP fluorescence K1497022 false false _1877_ 0 12466 9 It's complicated false d false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen component2398955 1 BBa_K1055000 component2398954 1 BBa_K1497019 annotation2398955 1 BBa_K1055000 range2398955 1 1221 1937 annotation2398954 1 BBa_K1497019 range2398954 1 1 1214 BBa_K1055000 1 BBa_K1055000 mKate is a fluorophor derived from dsRed 2013-09-17T11:00:00Z 2015-05-08T01:08:57Z IDT Technologies, gBLOCK assembly and subsequent PCR isolation mKate is a fluorophor derived from dsRed. It features the excitation maxima at 588 nm and the emission maxima at 633 nm. false false _1362_ 0 16536 9 It's complicated false Nucleotide sequence was added in order to avoid the formation of secondary structures during gBLOCK assembly and subsequent steps. The amino acid sequence was left unchanged. false Kai Fenzl BBa_K1497019_sequence 1 ctccttgtctcctaccctgcctggcggggtgtggccgctatcggcgcgttgtcatggccaggctcaacagcggcgcatccagtgcgagtgcgccgccggcttgccgcgccagccctttctggtggggccatgcagcggatgagtgaagcttaagagtgagcttgatattgatcaaatggattgttttgattcatgatatggacggcatcaatacattgaccaccccatccgtcgtctatccgagggtcggcaagaacaagcacaacaacggagaccagcgccatgcgtttcaacaagctcgacctcaatcttctggtcgccctggatgcactgctcacggagatgagcatcagccgcgccgccgaaaagatccatctgagccagtcggccatgagcaatgccctggcgcggctgcgcgagtatttcgatgatgaattgctgatccaggtgggccggcgcatggagcccacgccgcgcgccgaggtgctcaaggatgcggtgcatgatgtgctgcggcgtatcgatggctccatcgcggcgctgccggccttcgtgccggccgagtccacgcgcgagtttcgcatctcggtttcggactttacgctctccgtcctcatcccccgggtgctggcgcgcgcgcacgccgagggcaagcacatccgctttgccctgatgccgcaggtgcaagacccgacccgctcgctggatcgggccgaggtggacctgctggtcttgccgcaggaattatgcacgcccgatcatcctgccgaagaggtcttccgcgaacggcatgtctgcgtggtctggcgcgacagtgcgctggcgcaaggcgagctgacgctggaacgctacatggcctcaggccatgtggtgatggtgccgcctggggccaatgcgtcgtcggtggaggcgtggatggccaggaagctgggctttgcgcgccgggtggaagtgaccagcttcagcttcgcttctgcgctggcgctggtacaggggacggaccgcatcgccacggtgcatgcccggctggcgcagctgctggctccgcaatggccggtggtgatcaaggagagtccgctgtcgctgggcgagatgcggcagatgatgcagtggcatcgctaccgcagcaatgatcctggcatccagtggctgcgtcgggtgtttctggagagtgcgcaggagatggatgcggcgctgccaggcatctgctgaca BBa_K1055000_sequence 1 atggtaagcaaaggtgaagagttaatcaaggagaacatgcacatgaaattgtatatggaaggtacggtgaataatcaccatttcaaatgcacaagtgaaggcgaagggaagccgtacgaaggcacgcagacgatgcgcattaaggtagtggaaggcggtcctttaccgttcgcttttgatatcctggccacctccttcatgtatgggtccaagacctttatcaaccatacccagggcattccggacttttttaaacagtcttttccagaaggtttcacttgggagcgtgtaacgacctacgaggatggtggcgtgctgacggcaacccaggacacgagccttcaggatggatgtcttatttacaacgtgaaaattcgcggagtgaactttccgagcaacggtccagttatgcagaaaaaaaccctggggtgggaggcctcaaccgaaatgctgtacccagctgatggtggtctggaaggtcgctcggacatggcgctgaagctggtcggtggtggccatcttatttgtaaccttaaaacgacctaccgtagcaaaaagcctgcgaaaaacctgaaaatgcccggtgtctactacgttgaccggcgtctggaacgtattaaggaagccgacaaagagacatatgtggagcagcacgaagttgccgttgcacgctattgcgatctcccgagcaacctgggtcataaactgaattaataa BBa_K1497021_sequence 1 ctccttgtctcctaccctgcctggcggggtgtggccgctatcggcgcgttgtcatggccaggctcaacagcggcgcatccagtgcgagtgcgccgccggcttgccgcgccagccctttctggtggggccatgcagcggatgagtgaagcttaagagtgagcttgatattgatcaaatggattgttttgattcatgatatggacggcatcaatacattgaccaccccatccgtcgtctatccgagggtcggcaagaacaagcacaacaacggagaccagcgccatgcgtttcaacaagctcgacctcaatcttctggtcgccctggatgcactgctcacggagatgagcatcagccgcgccgccgaaaagatccatctgagccagtcggccatgagcaatgccctggcgcggctgcgcgagtatttcgatgatgaattgctgatccaggtgggccggcgcatggagcccacgccgcgcgccgaggtgctcaaggatgcggtgcatgatgtgctgcggcgtatcgatggctccatcgcggcgctgccggccttcgtgccggccgagtccacgcgcgagtttcgcatctcggtttcggactttacgctctccgtcctcatcccccgggtgctggcgcgcgcgcacgccgagggcaagcacatccgctttgccctgatgccgcaggtgcaagacccgacccgctcgctggatcgggccgaggtggacctgctggtcttgccgcaggaattatgcacgcccgatcatcctgccgaagaggtcttccgcgaacggcatgtctgcgtggtctggcgcgacagtgcgctggcgcaaggcgagctgacgctggaacgctacatggcctcaggccatgtggtgatggtgccgcctggggccaatgcgtcgtcggtggaggcgtggatggccaggaagctgggctttgcgcgccgggtggaagtgaccagcttcagcttcgcttctgcgctggcgctggtacaggggacggaccgcatcgccacggtgcatgcccggctggcgcagctgctggctccgcaatggccggtggtgatcaaggagagtccgctgtcgctgggcgagatgcggcagatgatgcagtggcatcgctaccgcagcaatgatcctggcatccagtggctgcgtcgggtgtttctggagagtgcgcaggagatggatgcggcgctgccaggcatctgctgacatactagatggtaagcaaaggtgaagagttaatcaaggagaacatgcacatgaaattgtatatggaaggtacggtgaataatcaccatttcaaatgcacaagtgaaggcgaagggaagccgtacgaaggcacgcagacgatgcgcattaaggtagtggaaggcggtcctttaccgttcgcttttgatatcctggccacctccttcatgtatgggtccaagacctttatcaaccatacccagggcattccggacttttttaaacagtcttttccagaaggtttcacttgggagcgtgtaacgacctacgaggatggtggcgtgctgacggcaacccaggacacgagccttcaggatggatgtcttatttacaacgtgaaaattcgcggagtgaactttccgagcaacggtccagttatgcagaaaaaaaccctggggtgggaggcctcaaccgaaatgctgtacccagctgatggtggtctggaaggtcgctcggacatggcgctgaagctggtcggtggtggccatcttatttgtaaccttaaaacgacctaccgtagcaaaaagcctgcgaaaaacctgaaaatgcccggtgtctactacgttgaccggcgtctggaacgtattaaggaagccgacaaagagacatatgtggagcagcacgaagttgccgttgcacgctattgcgatctcccgagcaacctgggtcataaactgaattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z