BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1497012 1 BBa_K1497012 B0034-F3H 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d d false false _1877_ 0 12466 9 It's complicated false d false Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky component2398820 1 BBa_K1497009 component2398819 1 BBa_B0034 annotation2398819 1 BBa_B0034 range2398819 1 1 12 annotation2398820 1 BBa_K1497009 range2398820 1 19 1125 BBa_K1497009 1 BBa_K1497009 F3H 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d d false false _1877_ 0 12466 9 In stock false d false Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky BBa_K1497011 1 BBa_K1497011 B0034-ANS 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d d false false _1877_ 0 12466 9 In stock false d false Sascha Hein, Sven Jager, Thomas Dohmen BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_K1497999 1 BBa_K1497999 T7-B0034-F3H-B0034-DFR-B0034-sANS 2014-10-06T11:00:00Z 2015-05-08T01:10:46Z s s false false _1877_ 0 12466 9 It's complicated false s false Sascha Hein component2398867 1 BBa_K1497011 component2398866 1 BBa_K1497013 component2398862 1 BBa_K1497012 component2398858 1 BBa_I712074 annotation2398866 1 BBa_K1497013 range2398866 1 1188 2267 annotation2398862 1 BBa_K1497012 range2398862 1 55 1179 annotation2398867 1 BBa_K1497011 range2398867 1 2276 3445 annotation2398858 1 BBa_I712074 range2398858 1 1 46 BBa_K1497013 1 BBa_K1497013 B0034-DFR 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z a a false false _1877_ 0 12466 9 In stock false a false Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky component2398837 1 BBa_K1497010 component2398836 1 BBa_B0034 annotation2398836 1 BBa_B0034 range2398836 1 1 12 annotation2398837 1 BBa_K1497010 range2398837 1 19 1080 BBa_K1497010 1 BBa_K1497010 Dihydroflavonol 4-reductase (DFR) 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d d false false _1877_ 0 12466 9 In stock false d false Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky BBa_K1497999_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatggctccttcaactctcactgccttggcccaagagaaaactctaaattctaaatttgttcgagatgaagatgagcgtcccaaaattgcctacaataaattcagcgacgaaatccctgtcatttctctagctggtattggtgatgattctgttgacaagagatcccagatctgtcgaaaaattgtcgaggcttgtgaagactgggggattttccaggtggttgatcacggtattgatatcgatttaatttctgaaatgactcgtctcgctcgacaattctttgccttgccagctgaggaaaaactccggtttgacatgactggtggtaagaaaggtggatttattgtctctagtcatcttcagggtgaagcagtgcaggattggcgtgagatagtggcttatttttcgtacccaattcaggcccgggactactcaagatggcctgataagccggagggatggaggtccatcacggagatgtacagtgacaagttgatggcactagcttgcaagttactagaagtgttatcagaggccatggggcttgaaaaagagggtcttacaaaggcgtgtgtggatatggaccagaaagtgatagttaattactatcccaagtgcccacaacctgacttgacactgggactcaaaagacatacggatcctggtactattacccttttgttacaggatcaggttggtgggttacaggctactagggatggcggcaaaacttggattactgttcagcctgtggagggagcttttgttgtcaatttgggtgatcatggtcactatttaagcaatgggaggttcaagaatgccgaccaccaagcagttgtgaactccaattctagcagaatgtcaattgcaactttccagaacccggctccgaatgcaacagtatatccattaaagattagggagggagagaaggccgttatggaggagcctattacatttgctgagatgtacaagaggaagatgagcagagatattgagatggctacgcttaagaaattggctaaagaaaaagtgttgcaagaccaagaagtggagaaggccaagctgcaaatgacacccaagagcgcagacgaaattttcgcttgatactagagaaagaggagaaatactagatggtttctagtacaattaacgagacactagacggtaaacatgacattaataaggttgggcaaggcgagaccgtctgcgtgaccggtgcatccggtttcattggttcatggctcatcatgcgactccttgagcgtggctacaccgttcgagccacagttcgtgatcccgataacactaaaaaagtgcaacatttgttggatttgccaaatgccaagactaacttgacactttggaaagcggacctacacgaagaaggcagctttgatgcagccgttgatggatgcaccggcgtgtttcatatcgccacacctatggactttgagtccaaggatcccgagaatgagatgataaaacctacaataaatggaatgttggacatactaaagtcatgtgtgaaggccaaagtaaggagggtggtttttacgtcatctggtggaactgtgaatgtcgaagcgactcaaaaacccgtctatgacgagacttgttggagtgatctcgacttcattcgctctgttaagatgactggatggatgtacttcgcgtcaaaaatactagcagagcaagcagcgtggaagtacgcagcagaaaacaatctcgaatttatcagtatcattccaccgctcgttgttgggccctttatcatgccttctatgcctcctagcctcattaccgcgttatcccccataacaagaactgaatcacattatacaataataaagcaaggacaattcgtgcacttggatgacctttgtatgtctcacatcttcttatacgagaatccgaaggcaaatggtcgatatattgcctcagcctgtgctgctaccatttacgacatcgcaaagatgctcagggaagaataccctgagtacaatgtccccaccaaattcaaggactacaaggaggtcatggggcaagtacaattctcgtcgaagaaactgacggatctcgggtttgagttcaaatacgggttgaaggacatgtacacagcagctgtcgagacttgcagagcaaaagggcttcttcctctttccctagaacatcatgaaaatggatctgcttaatactagaggaaagaggagaaaactagatggttaccgcagcaagcattggtagccgtgttgaaagcctggcaagcagcggtattagcaccattccgaaagaatatgttcgtccggaagaagaactggttaacattggcgatatcttcgaggatgaaaaaagcaccgaaggtccgcaggttccgaccattgatctgaaagaaattgatagcgacgatatcaaagtgcgcgaaaaatgtcgtgaggacctgaaagaagcagcagttgaatggggtgttatgcatctgatcaatcatggcattagtgatgaactgatggaacgcgttaaaaaagcaggcaaagcattttttgatctgccgatcgaacagaaagaaaaatatgcaaatgatcaggccagcggcaaaattcagggttatggtagcaaactggcaaataatgcaagcggtcagctggaatgggaagattacttttttcactgtgtgtacccggaagataaacgtgatctgagcatttggcctcagaccccgagcgattatattgttgcaaccagcgaatatgccaaagaactgcgtggtctggcaaccaaaattctgagtattctgagcctgggtctgggcctggaagaaggtcgtctggaaaaagaagttggcggtctggaagaactgctgctgcaaatgaaaattaactattacccgaaatgtccgcagccggaactggcactgggtgttgaagcacataccgatattagcgcactgacctttattctgcataatatggttccgggtctgcaactgttttatggtggtaaatgggttaccgccaaatgtgttccgaatagcattgtgatgcatattggtgataccctggaaattctgtccaacggtaaatacaaaagcattctgcatcgtggcctggtgaataaagaaaaagttcgtattagctgggcagtgttttgtgaaccgccaaaagaaaaaatcattctgaaaccgctgccggaaaccgttagcgaagaggaaccggcaatttttccgcctcgtaccttttttgaacacatccagcataaactgtttcgccagagccaagaagccctggttagcaccaaagaaagcgcagcactgaaaagcaccaccgaaagtgcactgaaatcaaccaaagaggcagccctgatttcaaccaattaa BBa_K1497010_sequence 1 atggtttctagtacaattaacgagacactagacggtaaacatgacattaataaggttgggcaaggcgagaccgtctgcgtgaccggtgcatccggtttcattggttcatggctcatcatgcgactccttgagcgtggctacaccgttcgagccacagttcgtgatcccgataacactaaaaaagtgcaacatttgttggatttgccaaatgccaagactaacttgacactttggaaagcggacctacacgaagaaggcagctttgatgcagccgttgatggatgcaccggcgtgtttcatatcgccacacctatggactttgagtccaaggatcccgagaatgagatgataaaacctacaataaatggaatgttggacatactaaagtcatgtgtgaaggccaaagtaaggagggtggtttttacgtcatctggtggaactgtgaatgtcgaagcgactcaaaaacccgtctatgacgagacttgttggagtgatctcgacttcattcgctctgttaagatgactggatggatgtacttcgcgtcaaaaatactagcagagcaagcagcgtggaagtacgcagcagaaaacaatctcgaatttatcagtatcattccaccgctcgttgttgggccctttatcatgccttctatgcctcctagcctcattaccgcgttatcccccataacaagaactgaatcacattatacaataataaagcaaggacaattcgtgcacttggatgacctttgtatgtctcacatcttcttatacgagaatccgaaggcaaatggtcgatatattgcctcagcctgtgctgctaccatttacgacatcgcaaagatgctcagggaagaataccctgagtacaatgtccccaccaaattcaaggactacaaggaggtcatggggcaagtacaattctcgtcgaagaaactgacggatctcgggtttgagttcaaatacgggttgaaggacatgtacacagcagctgtcgagacttgcagagcaaaagggcttcttcctctttccctagaacatcatgaaaatggatctgcttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1497012_sequence 1 aaagaggagaaatactagatggctccttcaactctcactgccttggcccaagagaaaactctaaattctaaatttgttcgagatgaagatgagcgtcccaaaattgcctacaataaattcagcgacgaaatccctgtcatttctctagctggtattggtgatgattctgttgacaagagatcccagatctgtcgaaaaattgtcgaggcttgtgaagactgggggattttccaggtggttgatcacggtattgatatcgatttaatttctgaaatgactcgtctcgctcgacaattctttgccttgccagctgaggaaaaactccggtttgacatgactggtggtaagaaaggtggatttattgtctctagtcatcttcagggtgaagcagtgcaggattggcgtgagatagtggcttatttttcgtacccaattcaggcccgggactactcaagatggcctgataagccggagggatggaggtccatcacggagatgtacagtgacaagttgatggcactagcttgcaagttactagaagtgttatcagaggccatggggcttgaaaaagagggtcttacaaaggcgtgtgtggatatggaccagaaagtgatagttaattactatcccaagtgcccacaacctgacttgacactgggactcaaaagacatacggatcctggtactattacccttttgttacaggatcaggttggtgggttacaggctactagggatggcggcaaaacttggattactgttcagcctgtggagggagcttttgttgtcaatttgggtgatcatggtcactatttaagcaatgggaggttcaagaatgccgaccaccaagcagttgtgaactccaattctagcagaatgtcaattgcaactttccagaacccggctccgaatgcaacagtatatccattaaagattagggagggagagaaggccgttatggaggagcctattacatttgctgagatgtacaagaggaagatgagcagagatattgagatggctacgcttaagaaattggctaaagaaaaagtgttgcaagaccaagaagtggagaaggccaagctgcaaatgacacccaagagcgcagacgaaattttcgcttga BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K1497009_sequence 1 atggctccttcaactctcactgccttggcccaagagaaaactctaaattctaaatttgttcgagatgaagatgagcgtcccaaaattgcctacaataaattcagcgacgaaatccctgtcatttctctagctggtattggtgatgattctgttgacaagagatcccagatctgtcgaaaaattgtcgaggcttgtgaagactgggggattttccaggtggttgatcacggtattgatatcgatttaatttctgaaatgactcgtctcgctcgacaattctttgccttgccagctgaggaaaaactccggtttgacatgactggtggtaagaaaggtggatttattgtctctagtcatcttcagggtgaagcagtgcaggattggcgtgagatagtggcttatttttcgtacccaattcaggcccgggactactcaagatggcctgataagccggagggatggaggtccatcacggagatgtacagtgacaagttgatggcactagcttgcaagttactagaagtgttatcagaggccatggggcttgaaaaagagggtcttacaaaggcgtgtgtggatatggaccagaaagtgatagttaattactatcccaagtgcccacaacctgacttgacactgggactcaaaagacatacggatcctggtactattacccttttgttacaggatcaggttggtgggttacaggctactagggatggcggcaaaacttggattactgttcagcctgtggagggagcttttgttgtcaatttgggtgatcatggtcactatttaagcaatgggaggttcaagaatgccgaccaccaagcagttgtgaactccaattctagcagaatgtcaattgcaactttccagaacccggctccgaatgcaacagtatatccattaaagattagggagggagagaaggccgttatggaggagcctattacatttgctgagatgtacaagaggaagatgagcagagatattgagatggctacgcttaagaaattggctaaagaaaaagtgttgcaagaccaagaagtggagaaggccaagctgcaaatgacacccaagagcgcagacgaaattttcgcttga BBa_K1497013_sequence 1 aaagaggagaaatactagatggtttctagtacaattaacgagacactagacggtaaacatgacattaataaggttgggcaaggcgagaccgtctgcgtgaccggtgcatccggtttcattggttcatggctcatcatgcgactccttgagcgtggctacaccgttcgagccacagttcgtgatcccgataacactaaaaaagtgcaacatttgttggatttgccaaatgccaagactaacttgacactttggaaagcggacctacacgaagaaggcagctttgatgcagccgttgatggatgcaccggcgtgtttcatatcgccacacctatggactttgagtccaaggatcccgagaatgagatgataaaacctacaataaatggaatgttggacatactaaagtcatgtgtgaaggccaaagtaaggagggtggtttttacgtcatctggtggaactgtgaatgtcgaagcgactcaaaaacccgtctatgacgagacttgttggagtgatctcgacttcattcgctctgttaagatgactggatggatgtacttcgcgtcaaaaatactagcagagcaagcagcgtggaagtacgcagcagaaaacaatctcgaatttatcagtatcattccaccgctcgttgttgggccctttatcatgccttctatgcctcctagcctcattaccgcgttatcccccataacaagaactgaatcacattatacaataataaagcaaggacaattcgtgcacttggatgacctttgtatgtctcacatcttcttatacgagaatccgaaggcaaatggtcgatatattgcctcagcctgtgctgctaccatttacgacatcgcaaagatgctcagggaagaataccctgagtacaatgtccccaccaaattcaaggactacaaggaggtcatggggcaagtacaattctcgtcgaagaaactgacggatctcgggtttgagttcaaatacgggttgaaggacatgtacacagcagctgtcgagacttgcagagcaaaagggcttcttcctctttccctagaacatcatgaaaatggatctgcttaa BBa_K1497011_sequence 1 gaaagaggagaaaactagatggttaccgcagcaagcattggtagccgtgttgaaagcctggcaagcagcggtattagcaccattccgaaagaatatgttcgtccggaagaagaactggttaacattggcgatatcttcgaggatgaaaaaagcaccgaaggtccgcaggttccgaccattgatctgaaagaaattgatagcgacgatatcaaagtgcgcgaaaaatgtcgtgaggacctgaaagaagcagcagttgaatggggtgttatgcatctgatcaatcatggcattagtgatgaactgatggaacgcgttaaaaaagcaggcaaagcattttttgatctgccgatcgaacagaaagaaaaatatgcaaatgatcaggccagcggcaaaattcagggttatggtagcaaactggcaaataatgcaagcggtcagctggaatgggaagattacttttttcactgtgtgtacccggaagataaacgtgatctgagcatttggcctcagaccccgagcgattatattgttgcaaccagcgaatatgccaaagaactgcgtggtctggcaaccaaaattctgagtattctgagcctgggtctgggcctggaagaaggtcgtctggaaaaagaagttggcggtctggaagaactgctgctgcaaatgaaaattaactattacccgaaatgtccgcagccggaactggcactgggtgttgaagcacataccgatattagcgcactgacctttattctgcataatatggttccgggtctgcaactgttttatggtggtaaatgggttaccgccaaatgtgttccgaatagcattgtgatgcatattggtgataccctggaaattctgtccaacggtaaatacaaaagcattctgcatcgtggcctggtgaataaagaaaaagttcgtattagctgggcagtgttttgtgaaccgccaaaagaaaaaatcattctgaaaccgctgccggaaaccgttagcgaagaggaaccggcaatttttccgcctcgtaccttttttgaacacatccagcataaactgtttcgccagagccaagaagccctggttagcaccaaagaaagcgcagcactgaaaagcaccaccgaaagtgcactgaaatcaaccaaagaggcagccctgatttcaaccaattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z