BBa_K1498000 1 BBa_K1498000 kerUS keratinase from Brevibacillus brevis 2014-10-06T11:00:00Z 2015-05-08T01:10:46Z is the codon optimised (for E. coli) kerUS gene from: Jaouadi, N. Z., Rekik, H., Badis, A., Trabelsi, S., Belhoul, M., Yahiaoui, A. B., ??? Jaouadi, B. (2013). Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities. PloS one, 8(10), e76722. doi:10.1371/journal.pone.0076722 Genbank Number: KC152966 Coding region of kerUS keratinase from Brevibacillus brevis with signal for extracellular secretion, which is cleaved following secretion Codes for a 45.9kDa (predicted)pre-processed protein, cleaved to 29kDa active protein false false _1878_ 0 21965 9 In stock false codon optimisation for E. coli, ensuring no BioBrick restriction enzyme sites were present false Charlotte Green BBa_K1498000_sequence 1 gattaaagaggagaaatactagatgtgcgtgaaaaaaaaaaacgtgatgaccagcgttctgctggcagttccgctgctgtttagcgcaggttttggtggtagcatggcaaatgcagaacgtgttagcaaaaccgatagcgagaaaagctatatcgttggttttaaagcaagcgcaaccaccaatagcagcaaaaaacaggcagttattcagaatggtggcaaactggaaaaacagtatcgtctgattaatgcagcacaggttaccatgagcgaacaggcagccaaaaaactggaacatgatccgagcattgcatatgtggaagaagatcataaagccgaagcatatgcacagaccgttccgtatggtattccgcagatcaaagaaccggcagttcatgcacagggttataaaggtgcaaatgttaaagttgcagttctggatacaggtattcatgcagcccatccggatctgaatgttgccggtggtgcaagctttaaaccgagcgaaccgaatgcaacccaggattttcagagccatgccacccatgttgcaggcaccattgcagcactggataataccattggtgttctgggtgttgcaccgagcgcaagcctgtatgcagttaaagtgctggatcgtaatggtgatggtcaggatagctggattattagcggtattgaatgggcagttgccaataacatggatgtgattaatatgagcctgggtggtccgaatggtagcaccgcactgaaaaatgcagttgatacagcaaataatcgtggtgttgttgttgtggcagcagcaggtaatagcggtagctttggtagtaccagcaccgttggttatccggcaaaatatgatagtaccggtgcagttgcgaatgtgaatagcaataatgttaataacagcagcagctcagcaggtccggaactggatgttagcgcaccgggtacaagcattctgagcaccgttccgagcagcggttataccagctataccgcaaccagcatggcatcaccgcatgttgcgggtgcagcagccgttattctgagtaaatatccgaatctgaccaatagccaggttcgtcagcgtctggaaaacaccgcaacaccgctgggtgatagcttctattatggtaaaggtctgatcaatgttcaggcagcgagcaattaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z