LOCUS       BBa_K1499203            1529 bp    DNA     linear       26-Nov-2024
DEFINITION  SdaB protein generator without tRNA + 5 amber stops
ACCESSION   BBa_K1499203
VERSION     BBa_K1499203.1  
FEATURES             Location/Qualifiers
     misc_feature    1..55
                     /label=J23038
     promoter        1..35
                     /label=Strong promoter (J23104)
     RBS             44..55
                     /label=B0034
     CDS             62..1429
                     /label=SdaB with 5 TAG stops
     misc_feature    158..160
                     /label=TAG stop 1
     misc_feature    749..751
                     /label=TAG stop 2
     misc_feature    866..868
                     /label=TAG stop 3
     misc_feature    995..997
                     /label=TAG stop 4
     misc_feature    1322..1324
                     /label=TAG stop 5
     stem_loop       1430..1509
                     /label=Terminator (B0010)
ORIGIN
        1 tgacagctag ctcagtccta ggtattgtgc tagctactag agaaagagga gaaagaccag
       61 atgattagcg tattcgatat tttcaaaatc ggcattggcc cttccagttc tcataccgtt
      121 ggaccaatga aagcgggtaa acaatttacc gacgattaga ttgcccgtaa cctgcttaaa
      181 gacgtgaccc gcgtggtggt tgacgtgtac ggctcgctct ctctgaccgg taaaggccac
      241 cacactgata tcgccattat tatgggcctg gcgggtaacc tgccggatac cgtggatatc
      301 gattccatcc ccagttttat tcaggatgtg aatactcatg gtcgcctgat gctggcaaac
      361 ggtcagcatg aagtggagtt cccggttgat cagtgcatga acttccacgc cgacaacctt
      421 tctctgcatg aaaacggtat gcgcattacc gcgctggcgg gcgataaagt cgtttacagc
      481 cagacttact actctattgg cggtggcttt atcgttgatg aagagcattt tggccagcag
      541 gatagcgcac cggttgaagt tccttatccg tacagttcag cagccgatct gcaaaaacat
      601 tgtcaggaaa ccgggctgtc actctctggc ctgatgatga aaaacgagct ggcgctgcac
      661 agcaaagaag agctggaaca gcacctggcg aacgtctggg aagtcatgcg cggcggtatt
      721 gagcgcggta tttccaccga aggcgtgtag cctggcaaac tgcgcgttcc acgccgtgct
      781 gcggcactac gccggatgct ggtcagccag gataaaacca ccactgaccc gatggcggtt
      841 gttgactgga tcaacatgtt tgcataggca gtgaacgaag agaacgctgc tggcggtcgc
      901 gtggtgactg cgccgactaa cggtgcgtgc gggattatcc cggcagttct ggcgtactac
      961 gacaagttta tccgcgaagt gaacgctaac tcataggctc gttacctgct ggtagccagc
     1021 gccattggtt ctctttataa gatgaacgcg tcgatttctg gtgctgaagt gggttgccag
     1081 ggtgaagttg gcgtggcgtg ctcaatggcg gcggctggtc tggcagaact attaggcgca
     1141 agcccggcgc aggtgtgcat cgcggcggaa atcgccatgg agcacaacct cggtctgacg
     1201 tgtgacccgg tcgccggaca ggtacaggtg ccatgcatcg agcgtaacgc cattgcggca
     1261 gtaaaagcgg tgaacgccgc acgtatggcg ctgcgccgta ccagcgagcc gcgcgtctgc
     1321 taggataaag ttatcgaaac catgtacgaa acaggtaaag atatgaacgc caagtaccgc
     1381 gaaacctctc gcggcggcct ggcaatgaag atcgttgcct gcgattaacc aggcatcaaa
     1441 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1501 acgctctcca attccaaccg cctgaacgt
//