LOCUS       BBa_J23038                55 bp    DNA     linear       16-Feb-2025
DEFINITION  [Pcon][RBS]
ACCESSION   BBa_J23038
VERSION     BBa_J23038.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaa
//
LOCUS       BBa_K1499252            1124 bp    DNA     linear       16-Feb-2025
DEFINITION  GFP with 2 stop codons generator + supP tRNA
ACCESSION   BBa_K1499252
VERSION     BBa_K1499252.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S04591
     misc_feature    117..119
                     /label=Start Codon
     misc_feature    831..836
                     /label=Double TAA Stop
     misc_feature    159..161
                     /label=TAG Amber Stop1
     misc_feature    471..473
                     /label=TAG Amber Stop2
     CDS             117..836
                     /label=GFP (E0040) Ambered
     misc_feature    56..110
                     /label=J23038
     misc_feature    837..916
                     /label=Terminator (B0010)
     promoter        56..90
                     /label=Strong promoter
     RBS             99..110
                     /label=Strong RBS
     primer_bind     917..936
                     /label=Internal amplif primer_R
     CDS             56..781
                     /label=BBa_K1499250
     stem_loop       793..836
                     /label=stem_loop
     misc_feature    782..861
                     /label=BBa_B0010
     terminator      782..861
                     /label=BBa_B0010
     primer_bind     862..881
                     /label=Internal primer bind_R 
     primer_bind     882..901
                     /label=Internal primer bind_F
     misc_feature    902..1124
                     /label=tRNA and flanking regions
     misc_feature    1002..1086
                     /label=supP tRNA
     misc_feature    1036..1038
                     /label=CUA anticodon
     misc_feature    862..1124
                     /label=BBa_K1499251
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaagacca
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca atttaggttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacctaggt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      841 tgtttgtcgg tgaacgctct ccaattccaa ccgcctgaac gggggctgaa cttcctgtac
      901 cgctgaaaat ttcagcactt agcgaggtgc gacgaagctg gcgcttgcat ggtggcgtgc
      961 gacaggtata atccacaacg ttttccgcat acctcttcag tgccgaagtg gcgaaatcgg
     1021 tagacgcagt tgattctaaa tcaaccgtag aaatacgtgc cggttcgagt ccggccttcg
     1081 gcaccaaaag tatgtaaata gacctcaact gaggtctttt ttcc
//