BBa_K1499250 1 BBa_K1499250 GFP (E0040) with two amber stop codons 2014-08-26T11:00:00Z 2015-05-08T01:10:46Z todo todo false false _1879_ 0 11095 9 It's complicated false todo false Raman Nelakanti annotation2411110 1 Terminator (B0010) range2411110 1 782 861 annotation2411133 1 Internal amplif primer_R range2411133 1 862 881 annotation2411131 1 Strong RBS range2411131 1 44 55 annotation2381648 1 GFP (E0040) Ambered range2381648 1 62 781 annotation2411103 1 J23038 range2411103 1 1 55 annotation2381645 1 Double TAA Stop range2381645 1 776 781 annotation2381644 1 Start Codon range2381644 1 62 64 annotation2381646 1 TAG Amber Stop1 range2381646 1 104 106 annotation2381647 1 TAG Amber Stop2 range2381647 1 416 418 annotation2411128 1 Strong promoter range2411128 1 1 35 BBa_J23038 1 BBa_J23038 [Pcon][RBS] 2006-07-31T11:00:00Z 2015-08-31T04:08:39Z Digest pSB1A2-RA2 from 1022 library (SpeI/AlwNI/PstI, Neb2, Large 1568 ) Paste into pSB1A2-B0034 (XbaI/AlwNI, Neb2, Small 566) Released HQ 2013 RA2 promoter from 1022 library with a ribosome binding site. false true _52_ 0 933 52 In stock false na false Samantha Liang component1893629 1 BBa_J23104 component1893631 1 BBa_B0034 annotation1893631 1 BBa_B0034 range1893631 1 44 55 annotation1893629 1 BBa_J23104 range1893629 1 1 35 BBa_J23104 1 BBa_J23104 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters replace later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_S04591 1 BBa_S04591 J23104:B0034 2011-09-14T11:00:00Z 2015-05-08T01:14:42Z Released HQ 2013 true false _9_ 0 9115 9 Discontinued false false Julia M??ller component2128645 1 BBa_B0034 component2128643 1 BBa_J23104 annotation2128645 1 BBa_B0034 range2128645 1 44 55 annotation2128643 1 BBa_J23104 range2128643 1 1 35 BBa_K1499252 1 BBa_K1499252 GFP with 2 stop codons generator + supP tRNA 2014-08-26T11:00:00Z 2015-05-08T01:10:46Z todo todo false false _1879_ 0 11095 9 It's complicated true todo false Raman Nelakanti component2381693 1 BBa_S04591 component2381702 1 BBa_K1499251 component2381700 1 BBa_B0010 component2381699 1 BBa_K1499250 annotation2381702 1 BBa_K1499251 range2381702 1 862 1124 annotation2381693 1 BBa_S04591 range2381693 1 1 55 annotation2381700 1 BBa_B0010 range2381700 1 782 861 annotation2381699 1 BBa_K1499250 range2381699 1 56 781 BBa_K1499251 1 BBa_K1499251 supP tRNA 2014-08-26T11:00:00Z 2015-05-08T01:10:46Z todo todo false false _1879_ 0 11095 9 It's complicated true todo false Raman Nelakanti annotation2430056 1 Internal primer bind_R range2430056 1 1 20 annotation2430058 1 tRNA and flanking regions range2430058 1 41 263 annotation2430060 1 CUA anticodon range2430060 1 175 177 annotation2430057 1 Internal primer bind_F range2430057 1 21 40 annotation2430059 1 supP tRNA range2430059 1 141 225 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1499250_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaagaccagatgcgtaaaggagaagaacttttcactggagttgtcccaatttaggttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacctaggttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctccaattccaaccgcctgaacg BBa_B0034_sequence 1 aaagaggagaaa BBa_J23038_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa BBa_K1499252_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaagaccagatgcgtaaaggagaagaacttttcactggagttgtcccaatttaggttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacctaggttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctccaattccaaccgcctgaacgggggctgaacttcctgtaccgctgaaaatttcagcacttagcgaggtgcgacgaagctggcgcttgcatggtggcgtgcgacaggtataatccacaacgttttccgcatacctcttcagtgccgaagtggcgaaatcggtagacgcagttgattctaaatcaaccgtagaaatacgtgccggttcgagtccggccttcggcaccaaaagtatgtaaatagacctcaactgaggtcttttttcc BBa_J23104_sequence 1 ttgacagctagctcagtcctaggtattgtgctagc BBa_S04591_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa BBa_K1499251_sequence 1 caattccaaccgcctgaacgggggctgaacttcctgtaccactgaaaatttcagcacttagcgaggtgcgacgaagctggcgcttgcatggtggcgtgcgacaggtataatccacaacgttttccgcatacctcttcagtgccgaagtggcgaaatcggtagacgcagttgattctaaatcaaccgtagaaatacgtgccggttcgagtccggccttcggcaccaaaagtatgtaaatagacctcaactgaggtcttttttcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z