LOCUS       BBa_K1499253            1103 bp    DNA     linear       25-Nov-2024
DEFINITION  aeBlue generator with 3 UAG stops + supP tRNA
ACCESSION   BBa_K1499253
VERSION     BBa_K1499253.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23104
     RBS             44..55
                     /label=B0034
     misc_feature    36..43
                     /label=RFC10 scar
     misc_feature    56..61
                     /label=spacer
     misc_feature    62..88
                     /label=FLAG-tag
     CDS             92..775
                     /label=aeBlue (K864401, re-codon optimized)
     misc_feature    260..262
                     /label=TAG stop
     misc_feature    416..418
                     /label=TAG stop 2
     misc_feature    596..598
                     /label=TAG stop 3
     misc_feature    776..793
                     /label=His-tag
     misc_feature    794..799
                     /label=double stop codon
     stem_loop       800..879
                     /label=Terminator (B0010)
     misc_feature    880..1103
                     /label=supP tRNA (K1499251)
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaagacca
       61 gatggactac aaggacgacg atgacaagct tgcgagttta gtgaaaaaag acatgtgcat
      121 taaaatgact atggagggta cagtgaatgg ccatcatttc aaatgcgtgg gtgaaggaga
      181 gggaaaaccg tttgagggta cccaagtgga gaaaattcgt attacggaag ggggtccgct
      241 gccgtttgca tatgacattt aggcgccgtg ctgtatgtac ggctcaaaga catttatcaa
      301 acacgtttcc ggtattcccg attatttcaa agagtccttt ccggaaggtt tcacctggga
      361 acgcacacaa atcttcgaag atggtggcta cctgaccatc catcaagata cctcgtagca
      421 gggtaacaac tttattttta aggtgaatgt aatcggtgca aactttccgg caaatggacc
      481 cgttatgcaa aaaaaaacgg cgggttggga accatgtgtg gaaatgctgt acccacgtga
      541 tggcgttctg tgtgggcaat ctctgatggc attgaagtgc acggatggga atcactagac
      601 gagtcacctg cgcacgacgt atcgctcccg taagccgtcc aacgccgtca atatgccaga
      661 atttcatttt ggtgaccatc gtatcgaaat cctgaaagcg gaacagggca aattttatga
      721 acagtacgag tccgctgtcg ctcgttattg tgaagcagcc cccagtaaac tgggacacca
      781 ccatcaccat cactaatgac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc gctgaaaatt tcagcactta
      901 gcgaggtgcg acgaagctgg cgcttgcatg gtggcgtgcg acaggtataa tccacaacgt
      961 tttccgcata cctcttcagt gccgaagtgg cgaaatcggt agacgcagtt gattctaaat
     1021 caaccgtaga aatacgtgcc ggttcgagtc cggccttcgg caccaaaagt atgtaaatag
     1081 acctcaactg aggtcttttt tcc
//