BBa_K1499253 1 BBa_K1499253 aeBlue generator with 3 UAG stops + supP tRNA 2014-10-07T11:00:00Z 2015-05-08T01:10:46Z iGEM todo false false _1879_ 0 11095 9 It's complicated true added 3 stop codons for the amberless system false Raman Nelakanti annotation2430048 1 aeBlue (K864401, re-codon optimized) range2430048 1 92 775 annotation2430045 1 RFC10 scar range2430045 1 36 43 annotation2430055 1 supP tRNA (K1499251) range2430055 1 880 1103 annotation2430047 1 FLAG-tag range2430047 1 62 88 annotation2430043 1 J23104 range2430043 1 1 35 annotation2430044 1 B0034 range2430044 1 44 55 annotation2430046 1 spacer range2430046 1 56 61 annotation2430054 1 Terminator (B0010) range2430054 1 800 879 annotation2430050 1 TAG stop 2 range2430050 1 416 418 annotation2430051 1 TAG stop 3 range2430051 1 596 598 annotation2430052 1 His-tag range2430052 1 776 793 annotation2430053 1 double stop codon range2430053 1 794 799 annotation2430049 1 TAG stop range2430049 1 260 262 BBa_K1499253_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaagaccagatggactacaaggacgacgatgacaagcttgcgagtttagtgaaaaaagacatgtgcattaaaatgactatggagggtacagtgaatggccatcatttcaaatgcgtgggtgaaggagagggaaaaccgtttgagggtacccaagtggagaaaattcgtattacggaagggggtccgctgccgtttgcatatgacatttaggcgccgtgctgtatgtacggctcaaagacatttatcaaacacgtttccggtattcccgattatttcaaagagtcctttccggaaggtttcacctgggaacgcacacaaatcttcgaagatggtggctacctgaccatccatcaagatacctcgtagcagggtaacaactttatttttaaggtgaatgtaatcggtgcaaactttccggcaaatggacccgttatgcaaaaaaaaacggcgggttgggaaccatgtgtggaaatgctgtacccacgtgatggcgttctgtgtgggcaatctctgatggcattgaagtgcacggatgggaatcactagacgagtcacctgcgcacgacgtatcgctcccgtaagccgtccaacgccgtcaatatgccagaatttcattttggtgaccatcgtatcgaaatcctgaaagcggaacagggcaaattttatgaacagtacgagtccgctgtcgctcgttattgtgaagcagcccccagtaaactgggacaccaccatcaccatcactaatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctccgctgaaaatttcagcacttagcgaggtgcgacgaagctggcgcttgcatggtggcgtgcgacaggtataatccacaacgttttccgcatacctcttcagtgccgaagtggcgaaatcggtagacgcagttgattctaaatcaaccgtagaaatacgtgccggttcgagtccggccttcggcaccaaaagtatgtaaatagacctcaactgaggtcttttttcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z