##gff-version 3
##sequence-region BBa_K1499253 1 1103
BBa_K1499253	.	engineered_region	880	1103	.	+	0	ID=annotation2430055;Name=supP tRNA (K1499251)
BBa_K1499253	.	stop_codon	794	799	.	+	0	ID=annotation2430053;Name=double stop codon
BBa_K1499253	.	stop_codon	596	598	.	+	0	ID=annotation2430051;Name=TAG stop 3
BBa_K1499253	.	promoter	1	35	.	+	0	ID=annotation2430043;Name=J23104
BBa_K1499253	.	stop_codon	260	262	.	+	0	ID=annotation2430049;Name=TAG stop
BBa_K1499253	.	CDS	92	775	.	+	0	ID=annotation2430048;Name=aeBlue (K864401, re-codon optimized)
BBa_K1499253	.	sequence_feature	56	61	.	+	0	ID=annotation2430046;Name=spacer
BBa_K1499253	.	null	36	43	.	+	0	ID=annotation2430045;Name=RFC10 scar
BBa_K1499253	.	tag	62	88	.	+	0	ID=annotation2430047;Name=FLAG-tag
BBa_K1499253	.	stop_codon	416	418	.	+	0	ID=annotation2430050;Name=TAG stop 2
BBa_K1499253	.	ribosome_entry_site	44	55	.	+	0	ID=annotation2430044;Name=B0034
BBa_K1499253	.	tag	776	793	.	+	0	ID=annotation2430052;Name=His-tag
BBa_K1499253	.	stem_loop	800	879	.	+	0	ID=annotation2430054;Name=Terminator (B0010)
>BBa_K1499253
ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaagaccagatggactacaaggacgac
gatgacaagcttgcgagtttagtgaaaaaagacatgtgcattaaaatgactatggagggtacagtgaatggccatcatt
tcaaatgcgtgggtgaaggagagggaaaaccgtttgagggtacccaagtggagaaaattcgtattacggaagggggtcc
gctgccgtttgcatatgacatttaggcgccgtgctgtatgtacggctcaaagacatttatcaaacacgtttccggtatt
cccgattatttcaaagagtcctttccggaaggtttcacctgggaacgcacacaaatcttcgaagatggtggctacctga
ccatccatcaagatacctcgtagcagggtaacaactttatttttaaggtgaatgtaatcggtgcaaactttccggcaaa
tggacccgttatgcaaaaaaaaacggcgggttgggaaccatgtgtggaaatgctgtacccacgtgatggcgttctgtgt
gggcaatctctgatggcattgaagtgcacggatgggaatcactagacgagtcacctgcgcacgacgtatcgctcccgta
agccgtccaacgccgtcaatatgccagaatttcattttggtgaccatcgtatcgaaatcctgaaagcggaacagggcaa
attttatgaacagtacgagtccgctgtcgctcgttattgtgaagcagcccccagtaaactgggacaccaccatcaccat
cactaatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctccgctgaaaatttcagcacttagcgaggtgcgacgaagctggcgcttgcatggtggcgtgcgacaggtat
aatccacaacgttttccgcatacctcttcagtgccgaagtggcgaaatcggtagacgcagttgattctaaatcaaccgt
agaaatacgtgccggttcgagtccggccttcggcaccaaaagtatgtaaatagacctcaactgaggtcttttttcc