LOCUS BBa_K1499500 2756 bp DNA linear 09-May-2024 DEFINITION quorum sensing machinery that activates GFP expression ACCESSION BBa_K1499500 VERSION BBa_K1499500.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..666 /label=LuxI CDS 82..666 /label=BBa_C0161 stem_loop 686..729 /label=stem_loop misc_feature 675..754 /label=BBa_B0010 terminator 675..754 /label=BBa_B0010 stem_loop 770..789 /label=T7 TE misc_feature 796..796 /label=stop polyA_site 790..803 /label=polya misc_feature 763..803 /label=BBa_B0012 terminator 763..803 /label=BBa_B0012 misc_feature 1..803 /label=BBa_I13202 misc_feature 812..830 /label=TetR 1 misc_feature 812..865 /label=BBa_R0040 promoter 831..836 /label=-35 misc_feature 837..855 /label=TetR 2 promoter 854..859 /label=-10 promoter 812..865 /label=BBa_R0040 misc_feature 878..881 /label=conserved RBS 874..885 /label=BBa_B0034 misc_feature 892..893 /label=prefix misc_feature 1065..1065 /label=T misc_feature 1383..1383 /label=A CDS 892..1641 /label=luxR misc_feature 892..1647 /label=BBa_C0062 misc_feature 1648..1672 /label=Help:Barcodes CDS 892..1647 /label=BBa_C0062 stem_loop 1692..1735 /label=stem_loop misc_feature 1681..1760 /label=BBa_B0010 terminator 1681..1760 /label=BBa_B0010 stem_loop 1776..1795 /label=T7 TE misc_feature 1802..1802 /label=stop polyA_site 1796..1809 /label=polya misc_feature 1769..1809 /label=BBa_B0012 terminator 1769..1809 /label=BBa_B0012 misc_feature 1818..1837 /label=LuxR/HSL promoter 1837..1842 /label=-35 promoter 1859..1864 /label=-10 misc_feature 1870..1870 /label=start misc_feature 1818..1872 /label=BBa_R0062 promoter 1818..1872 /label=BBa_R0062 RBS 1881..1893 /label=RBS-3\Weak RBS 1887..1890 /label=RBS misc_feature 1881..1893 /label=BBa_B0032 RBS 1881..1893 /label=BBa_B0032 CDS 1900..2619 /label=GFP protein CDS 1900..2619 /label=BBa_E0040 stem_loop 2639..2682 /label=stem_loop misc_feature 2628..2707 /label=BBa_B0010 terminator 2628..2707 /label=BBa_B0010 stem_loop 2723..2742 /label=T7 TE misc_feature 2749..2749 /label=stop polyA_site 2743..2756 /label=polya misc_feature 2716..2756 /label=BBa_B0012 terminator 2716..2756 /label=BBa_B0012 misc_feature 812..2756 /label=BBa_T9002 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 721 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 781 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc 841 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatgaaaaac 901 ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat 961 gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc 1021 gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct 1081 aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat 1141 tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat 1201 aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt 1261 ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa 1321 gacaactata tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct 1381 tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta 1441 accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt 1501 tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg 1561 aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt 1621 gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc actactagag 1681 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1741 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1801 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 1861 tagtcgaata aatactagag tcacacagga aagtactaga tgcgtaaagg agaagaactt 1921 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 1981 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt 2041 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt 2101 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc 2161 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag 2221 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt 2281 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca 2341 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt 2401 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca 2461 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt 2521 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct 2581 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat 2641 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2701 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //