##gff-version 3
##sequence-region BBa_K1499500 1 2756
BBa_K1499500	.	engineered_region	1	803	.	+	0	ID=BBa_I13202;Name=BBa_I13202
BBa_K1499500	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13202
BBa_K1499500	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1499500	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I13202
BBa_K1499500	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1499500	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1499500	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1499500	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1499500	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1499500	.	terminator	675	754	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13202
BBa_K1499500	.	engineered_region	675	754	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1499500	.	stem_loop	686	729	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1499500	.	terminator	763	803	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13202
BBa_K1499500	.	stop_codon	796	796	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1499500	.	polyA_site	790	803	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1499500	.	stem_loop	770	789	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1499500	.	engineered_region	763	803	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1499500	.	CDS	82	666	.	+	0	ID=BBa_C0161;Name=BBa_C0161;Parent=BBa_I13202
BBa_K1499500	.	CDS	82	666	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
BBa_K1499500	.	engineered_region	812	2756	.	+	0	ID=BBa_T9002;Name=BBa_T9002
BBa_K1499500	.	ribosome_entry_site	874	885	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_T9002
BBa_K1499500	.	conserved_region	878	881	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1499500	.	promoter	812	865	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_T9002
BBa_K1499500	.	engineered_region	812	865	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1499500	.	promoter	831	836	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1499500	.	promoter	854	859	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1499500	.	non_covalent_binding_site	812	830	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1499500	.	non_covalent_binding_site	837	855	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1499500	.	terminator	2628	2707	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_K1499500	.	engineered_region	2628	2707	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1499500	.	stem_loop	2639	2682	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1499500	.	terminator	1681	1760	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_K1499500	.	engineered_region	1681	1760	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1499500	.	stem_loop	1692	1735	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1499500	.	ribosome_entry_site	1881	1893	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_T9002
BBa_K1499500	.	ribosome_entry_site	1881	1893	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1499500	.	engineered_region	1881	1893	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1499500	.	ribosome_entry_site	1887	1890	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1499500	.	CDS	892	1647	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_T9002
BBa_K1499500	.	engineered_tag	1648	1672	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1499500	.	engineered_region	892	1647	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1499500	.	engineered_region	892	893	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1499500	.	point_mutation	1065	1065	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1499500	.	CDS	892	1641	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1499500	.	point_mutation	1383	1383	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1499500	.	terminator	1769	1809	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_K1499500	.	stop_codon	1802	1802	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1499500	.	polyA_site	1796	1809	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1499500	.	stem_loop	1776	1795	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1499500	.	engineered_region	1769	1809	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1499500	.	terminator	2716	2756	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_K1499500	.	stop_codon	2749	2749	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1499500	.	polyA_site	2743	2756	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1499500	.	stem_loop	2723	2742	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1499500	.	engineered_region	2716	2756	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1499500	.	promoter	1818	1872	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_T9002
BBa_K1499500	.	start_codon	1870	1870	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1499500	.	promoter	1859	1864	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1499500	.	promoter	1837	1842	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1499500	.	engineered_region	1818	1872	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1499500	.	non_covalent_binding_site	1818	1837	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1499500	.	CDS	1900	2619	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_T9002
BBa_K1499500	.	CDS	1900	2619	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K1499500
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactact
agagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgt
agaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatca
tttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatga
cgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaa
aacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcc
ctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatt
tttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataat
aaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatattt
caaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccg
ctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgc
tagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata