BBa_K1499501 1 BBa_K1499501 Endo-1,4-beta-glucanase (cellulase gene) 2014-09-28T11:00:00Z 2015-05-08T01:10:46Z Genomic sequence of Neisseria Sicca SB Endo-1,4-&-beta-glucanase (EG 1) is an enzyme that breaks down the 1,4-beta-D-glycosidic linkages in cellulose to produce glucose. The coding sequence of this gene was from Neisseria Sicca bacterium that uses cellulose acetate as its sole carbon source. This enzyme is specific for 1,4-beta linkages in cellulose. false false _1879_ 0 22072 9 In stock false Since the gene is usually secreted by the original bacterium (Neisseria Sicca), it contains a signal sequence for extra-cellular secretion. We removed this signal sequence and replaced it with a secretion tag specific for E.coli, so that the enzyme can be secreted extracellularly. In order to allow protein purification and assays, we added a His-tag at the C' terminus of the gene. false Jovita Byemerwa annotation2430103 1 Metal-affinity tag range2430103 1 1378 1398 annotation2412319 1 Cellulase range2412319 1 1 1374 BBa_K1499501_sequence 1 atgtccgcccgccgcactgcctccgccctgttgaccgccgctgctttatccgttactggcctgaccgcggtccgtgagcatcgcgacgcagccgatggctgccgtgttgattataccgtgcaaagccagtggaacagcggtttccagggtgacgtgaagattacgaacctgggcagccctctgagctcttggacggtcgcatggacctttccatccagcggtcaacgcgtcagccagtcgtggaacgccaatgttacgaccagcggcagccgtgttagcgcgagcagcattggctggaatggttcgctgggcaccggtgccagcacccaattcggcttcattggttctttcaccgacagcaatccggttccgaccagctttaccgttaacggtgtagcgtgcacgggtaccgtcacgccgacccagggtccgacgcaaggtccgacccaaggcccaacccagggcccgacccagggtccgacgcagggtccgacgcaaggtccgactcaaggcccgacccagaacccgcagcctagcggcgatctgtacgtggacactgagaaccagagctacgcagcgtggcaagcggcgagcggtagcacgaaagaccttttggcgaagattgcattgacgccgcagagcgtgtggattggtaactggaactctccgagcgtgagccagcaacaaatgcgtgacgctaccagccgtgcgtccgctgcgggcaaaaccgtttctgtggtgatctacgcaatcccgggtcgtgactgtggtagctactccggtggccgtgtcgcggaaagcgaatatgcacaatggattgataccgtcgcgagcggtatcgtcggtaaaccaatcgtgatcctggagccggacgctctggcacaactgggcaattgcggtggccagggtgatcgcgttggttatctgcgctatgcggcaaaatccctgacggcagctggcggcgtttacatcgacgcgggtcacagcggctggttgagcgtggataccgcggttaatcgtctgaaccagatcggttttcaatatgcgctggcattcgcgctgaacaccagcaattaccaaacgacctcagcgagccagcaatacggtgaacagattagccagcgcctgggtggcaagggttatgttatcgacacctctcgcaatggtaatggttccaatggcgagtggtgcaaccgtggtcgtgcactgggcgataagccgcgtctggtgaacgacagcacgggcctggatgccctgctgtgggtcaaactgccgggtgagtctgacggtacgtgtaaacgtccggctgcgggtcagtggtggcaagatatggcgctggaatacgcccgtaatgcgaagtggccgggtattaagcataatcaccgtcacaagcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z