BBa_K1499502 1 BBa_K1499502 Cellulose Acetate Esterase (Cae) 2014-09-29T11:00:00Z 2015-05-08T01:10:46Z Genomic sequence of Neisseria Sicca Cellulose Acetate Esterase is an enzyme that de-acetylates cellulose acetate by hydrolyzing the acetyl ester moieties in cellulose acetate. The gene sequence is obtained from a bacterium, Neisseria Sicca that uses cellulose acetate as its sole source of carbon. Purified Cellulose Acetate Esterase has a high activity on Cellulose Acetate, but it has been shown to have activity on p-nitrophenyl acetate and some other p-nitrophenyl esters. (Original paper https://www.jstage.jst.go.jp/article/bbb/63/10/63_10_1708/_pdf) Since industrially made cellulose acetate is a plastic that tends to take a long time to biodegrade in the environment, the use of this enzyme could help in rapid degradation of cellulose acetate by removing the acetate groups. This part could be used specifically for de-acetylating cellulose acetate and other related esters. false false _1879_ 0 22072 9 Not in stock false Neisseria Sicca secretes the enzyme extracellularly and hence it has a signal sequence to aid for secretion. In order to use the gene in E.coli, we removed the signal sequence of Neisseria Sicca and added an E.coli specific secretion tag. In addition to that, we added a his-tag at the c'terminus end of the protein to aid purification of the protein. false Jovita Byemerwa annotation2429309 1 His Tag range2429309 1 1447 1468 annotation2429305 1 Signal sequence range2429305 1 94 184 annotation2429308 1 Esterase range2429308 1 189 1446 BBa_K1499502_sequence 1 aattcactcaggctcatcgcggcagaattcgcggccgcttctagattataggtaatacgactcactatagggttaactttaagaaggagaattatgaataacaatgacctgtttcaggcttcccgccgtcgcttcctggcacagttgggcgggttaacagtggccgggatgctcggtcctagtctgctgaaccctgggggtttgggcatgtatttatatgtgcctgacagcgtaaaacctaatgcgccagttttggtcgttgtgcattattgtactggcagtgctcaggctatgttttctgggacccaatttgacgaactggcgaaccggtacggctatattgttgtatatccgcaaccgacgggtcggggtgcctgctttgacgtaagtagtgcccaggcattacgtcatgacggtggctccgatcctacgtctattgcaaatatggttcgctacgtccagcgcacgtactctacggatgcttctcgtgtgttcgtaaccggtaccagctctggtgcaatgaccacccaagtcatgcttgcaacgtacccggatatttttgcggctggggcagcatttgcaggggtgccggccacatgcttttcgaccagctcgccgccgccgggatccacgagtcaggccccttggaactcagattgcgcccagggacgtattaatcgcacgggccagcagtggggcgatttggcgcgcgccgcttatccggggtataccggtgcccgcccccgggtccagctttggcatggcacgcaggatacgaccctggcctatgcaaactttggcgaagccgttgagcagtggacgaatgtgctcggcgtctctgctaccccgagcagtaccagtgtgaatggtgtagtcacacgcacacgctatggcggcacgggtgatcaagcaccggtggaggcaaactcgttagcaaacactacgcataatcttcctgtggacgcggcggcagctattcatttttttggcttagatggcccagcgccgacccagagccccacaagctctccacacccgactgatagcgcatcgccgaccccgacaccgacgccgacaccaactccgacgagttctgcctccgtcacgcccactggtaccgcgaccaccactgtggcatcaggatgtcgcgtctcgtaccaggtaaatgcatggaataccgggtacacagctaacgtcacgatcgggaattggggagccacggcggttgatggctggacgctgcgtttcaccctgcctgcgggacagcgtgtagagcaggcgtggtcctctcaggtaactcaaagcggtcaggtggttactgttactaatgcaccatggaacgctacgatcccggccggaggtaacgtccagcttggcttcaatgctagccatgatggttcggccgcggcgcctaccagcttcacgttgaacggcatgctgtgcgcgcacaaccaccgtcacaaacactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttactagtagcggccgctgcagtactgctaccgtcctgcctat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z