LOCUS BBa_K1499503 2893 bp DNA linear 23-Dec-2024 DEFINITION quorum sensing machinery that activates GFP expression with extra terminator ACCESSION BBa_K1499503 VERSION BBa_K1499503.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..666 /label=LuxI CDS 82..666 /label=BBa_C0161 stem_loop 686..729 /label=stem_loop misc_feature 675..754 /label=BBa_B0010 terminator 675..754 /label=BBa_B0010 stem_loop 770..789 /label=T7 TE misc_feature 796..796 /label=stop polyA_site 790..803 /label=polya misc_feature 763..803 /label=BBa_B0012 terminator 763..803 /label=BBa_B0012 misc_feature 812..830 /label=TetR 1 misc_feature 812..865 /label=BBa_R0040 promoter 831..836 /label=-35 misc_feature 837..855 /label=TetR 2 promoter 854..859 /label=-10 promoter 812..865 /label=BBa_R0040 misc_feature 878..881 /label=conserved RBS 874..885 /label=BBa_B0034 misc_feature 892..893 /label=prefix misc_feature 1065..1065 /label=T misc_feature 1383..1383 /label=A CDS 892..1641 /label=luxR misc_feature 892..1647 /label=BBa_C0062 misc_feature 1648..1672 /label=Help:Barcodes CDS 892..1647 /label=BBa_C0062 stem_loop 1692..1735 /label=stem_loop misc_feature 1681..1760 /label=BBa_B0010 terminator 1681..1760 /label=BBa_B0010 stem_loop 1776..1795 /label=T7 TE misc_feature 1802..1802 /label=stop polyA_site 1796..1809 /label=polya misc_feature 1769..1809 /label=BBa_B0012 terminator 1769..1809 /label=BBa_B0012 stem_loop 1829..1872 /label=stem_loop misc_feature 1818..1897 /label=BBa_B0010 terminator 1818..1897 /label=BBa_B0010 stem_loop 1913..1932 /label=T7 TE misc_feature 1939..1939 /label=stop polyA_site 1933..1946 /label=polya misc_feature 1906..1946 /label=BBa_B0012 terminator 1906..1946 /label=BBa_B0012 misc_feature 1955..1974 /label=LuxR/HSL promoter 1974..1979 /label=-35 promoter 1996..2001 /label=-10 misc_feature 2007..2007 /label=start misc_feature 1955..2009 /label=BBa_R0062 promoter 1955..2009 /label=BBa_R0062 RBS 2018..2030 /label=RBS-3\Weak RBS 2024..2027 /label=RBS misc_feature 2018..2030 /label=BBa_B0032 RBS 2018..2030 /label=BBa_B0032 CDS 2037..2756 /label=GFP protein CDS 2037..2756 /label=BBa_E0040 stem_loop 2776..2819 /label=stem_loop misc_feature 2765..2844 /label=BBa_B0010 terminator 2765..2844 /label=BBa_B0010 stem_loop 2860..2879 /label=T7 TE misc_feature 2886..2886 /label=stop polyA_site 2880..2893 /label=polya misc_feature 2853..2893 /label=BBa_B0012 terminator 2853..2893 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 721 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 781 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc 841 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatgaaaaac 901 ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat 961 gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc 1021 gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct 1081 aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat 1141 tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat 1201 aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt 1261 ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa 1321 gacaactata tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct 1381 tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta 1441 accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt 1501 tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg 1561 aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt 1621 gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc actactagag 1681 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1741 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1801 gcgtttatat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac aggtttacgc 1981 aagaaaatgg tttgttatag tcgaataaat actagagtca cacaggaaag tactagatgc 2041 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 2101 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 2161 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 2221 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg 2281 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 2341 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 2401 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 2461 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 2521 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 2581 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 2641 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 2701 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact 2761 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2821 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2881 ttctgcgttt ata //