##gff-version 3
##sequence-region BBa_K1499503 1 2893
BBa_K1499503	.	ribosome_entry_site	2018	2030	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1499503	.	ribosome_entry_site	2018	2030	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1499503	.	engineered_region	2018	2030	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1499503	.	ribosome_entry_site	2024	2027	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1499503	.	terminator	2765	2844	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1499503	.	engineered_region	2765	2844	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1499503	.	stem_loop	2776	2819	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1499503	.	promoter	1955	2009	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1499503	.	non_covalent_binding_site	1955	1974	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1499503	.	promoter	1974	1979	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1499503	.	engineered_region	1955	2009	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1499503	.	promoter	1996	2001	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1499503	.	start_codon	2007	2007	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1499503	.	terminator	1906	1946	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1499503	.	engineered_region	1906	1946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1499503	.	stem_loop	1913	1932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1499503	.	polyA_site	1933	1946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1499503	.	stop_codon	1939	1939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1499503	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1499503	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1499503	.	terminator	1769	1809	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1499503	.	engineered_region	1769	1809	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1499503	.	stem_loop	1776	1795	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1499503	.	polyA_site	1796	1809	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1499503	.	stop_codon	1802	1802	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1499503	.	terminator	1818	1897	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1499503	.	engineered_region	1818	1897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1499503	.	stem_loop	1829	1872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1499503	.	terminator	763	803	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1499503	.	engineered_region	763	803	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1499503	.	stem_loop	770	789	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1499503	.	polyA_site	790	803	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1499503	.	stop_codon	796	796	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1499503	.	CDS	892	1647	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1499503	.	engineered_region	892	893	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1499503	.	engineered_region	892	1647	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1499503	.	engineered_tag	1648	1672	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1499503	.	point_mutation	1383	1383	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1499503	.	point_mutation	1065	1065	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1499503	.	CDS	892	1641	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1499503	.	CDS	2037	2756	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K1499503	.	CDS	2037	2756	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1499503	.	terminator	675	754	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1499503	.	engineered_region	675	754	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1499503	.	stem_loop	686	729	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1499503	.	ribosome_entry_site	874	885	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1499503	.	conserved_region	878	881	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1499503	.	CDS	82	666	.	+	0	ID=BBa_C0161;Name=BBa_C0161
BBa_K1499503	.	CDS	82	666	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
BBa_K1499503	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1499503	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1499503	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1499503	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1499503	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1499503	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1499503	.	terminator	1681	1760	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1499503	.	engineered_region	1681	1760	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1499503	.	stem_loop	1692	1735	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1499503	.	terminator	2853	2893	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1499503	.	engineered_region	2853	2893	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1499503	.	stem_loop	2860	2879	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1499503	.	polyA_site	2880	2893	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1499503	.	stop_codon	2886	2886	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1499503	.	promoter	812	865	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1499503	.	promoter	854	859	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1499503	.	promoter	831	836	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1499503	.	engineered_region	812	865	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1499503	.	non_covalent_binding_site	837	855	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1499503	.	non_covalent_binding_site	812	830	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K1499503
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactact
agagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgt
agaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatca
tttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatga
cgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaa
aacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcc
ctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatt
tttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataat
aaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatattt
caaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccg
ctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgc
tagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata