LOCUS       BBa_K1500004            1705 bp    DNA     linear       22-Dec-2024
DEFINITION  IPTG-GFP MutD
ACCESSION   BBa_K1500004
VERSION     BBa_K1500004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..946
                     /label=GFP protein
     CDS             227..946
                     /label=BBa_E0040
     misc_feature    1..946
                     /label=BBa_K1500002
     RBS             955..967
                     /label=RBS-3\Weak
     RBS             961..964
                     /label=RBS
     misc_feature    955..967
                     /label=BBa_B0032
     RBS             955..967
                     /label=BBa_B0032
     CDS             974..1705
                     /label=MutD
     CDS             974..1705
                     /label=BBa_K1500998
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagtcacac
      961 aggaaagtac tagatgagca ctgcaattac acgccagatc gttctcgata ccgaaaccac
     1021 cggtatgaac cagattggtg cgcactatga aggccacaag atcattgaga ttggtgccgt
     1081 tgaagtggtg aaccgtcgcc tgacgggcaa taacttccat gtttatctca aacccgatcg
     1141 gctggtggat ccggaagcct ttggcgtaca tggtattgcc gatgaatttt tgctcgataa
     1201 gcccacgttt gccgaagtag ccgatgagtt catggactat attcgcggcg cggagttggt
     1261 gatccataac gcagcgttcg atatcggctt tatggactac gagttttcgt tgcttaagcg
     1321 cgatattccg aagaccaata ctttctgtaa ggtcaccgat agccttgcgg tggcgaggaa
     1381 aatgtttccc ggtaagcgca acagcctcga tgcgttatgt gctcgctacg aaatagataa
     1441 cagtaaacga acgctgcacg gggcattact cgatgcccag atccttgcgg aagtttatct
     1501 ggcgatgacc ggtggtcaaa cgtcgatggc ttttgcgatg gaaggagaga cacaacagca
     1561 acaaggtgaa gcaacaattc agcgcattgt acgtcaggca agtaagttac gcgttgtttt
     1621 tgcgacagat gaagagattg cagctcatga agcccgtctc gatctggtgc agaagaaagg
     1681 cggaagttgc ctctggcgag cataa
//