LOCUS       BBa_K1500006            2821 bp    DNA     linear       22-Nov-2024
DEFINITION  IPTG-GFP MutL
ACCESSION   BBa_K1500006
VERSION     BBa_K1500006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..946
                     /label=GFP protein
     CDS             227..946
                     /label=BBa_E0040
     misc_feature    1..946
                     /label=BBa_K1500002
     RBS             955..967
                     /label=RBS-3\Weak
     RBS             961..964
                     /label=RBS
     misc_feature    955..967
                     /label=BBa_B0032
     RBS             955..967
                     /label=BBa_B0032
     CDS             974..2821
                     /label=MutL
     CDS             974..2821
                     /label=BBa_K1500997
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagtcacac
      961 aggaaagtac tagatgccaa ttcaggtctt accgccacaa ctggcgaacc agattgccgc
     1021 aggtgaggtg gtcgagcgac ctgcgtcggt agtcaaagaa ctagtggaaa acagcctcga
     1081 tgcaggtgcg acgcgtatcg atattgatat cgaacgcggt ggggcgaaac ttatccgcat
     1141 tcgtgataac ggctgcggta tcaaaaaaga tgagctggcg ctggcgctgg ctcgtcatgc
     1201 caccagtaaa atcgcctctc tggacgatct cgaagccatt atcagcctgg gctttcgcgg
     1261 tgaggcgctg gcgagtatca gttcggtttc ccgcctgacg ctcacttcac gcaccgcaga
     1321 acagcaggaa gcctggcagg cctatgccga agggcgcgat atgaacgtga cggtaaaacc
     1381 ggcggcgcat cctgtgggga cgacgctgga ggtgctggat ctgttctaca acaccccggc
     1441 gcggcgcaaa ttcctgcgca ccgagaaaac cgaatttaac cacattgatg agatcatccg
     1501 ccgcattgcg ctggcgcgtt tcgacgtcac gatcaacctg tcgcataacg gtaaaattgt
     1561 gcgtcagtac cgcgcagtgc cggaaggcgg gcaaaaagaa cggcgcttag gcgcgatttg
     1621 cggcaccgct tttcttgaac aagcgctggc gattgaatgg caacacggcg atctcacgct
     1681 acgcggctgg gtggccgatc caaatcacac cacgcccgca ctggcagaaa ttcagtattg
     1741 ctacgtgaac ggtcgcatga tgcgcgatcg cctgatcaat cacgcgatcc gccaggcctg
     1801 cgaagacaaa ctgggggccg atcagcaacc ggcatttgtg ttgtatctgg agatcgaccc
     1861 acatcaggtg gacgtcaacg tgcaccccgc caaacacgaa gtgcgtttcc atcagtcgcg
     1921 tctggtgcat gattttatct atcagggcgt gctgagcgtg ctacaacagc aactggaaac
     1981 gccgctaccg ctggacgatg aaccccaacc tgcaccgcgt tccattccgg aaaaccgcgt
     2041 ggcggcgggg cgcaatcact ttgcagaacc ggcagctcgt gagccggtag ctccgcgcta
     2101 cactcctgcg ccagcatcag gcagtcgtcc ggctgccccc tggccgaatg cgcagccagg
     2161 ctaccagaaa cagcaaggtg aagtgtatcg ccagcttttg caaacgcccg cgccgatgca
     2221 aaaattaaaa gcgccggaac cgcaggaacc tgcacttgcg gcgaacagtc agagttttgg
     2281 tcgggtactg actatcgtcc attccgactg tgcgttgctg gagcgcgacg gcaacatttc
     2341 acttttatcc ttgccagtgg cagaacgttg gctgcgtcag gcacaattga cgccgggtga
     2401 agcgcccgtt tgcgcccagc cgctgctgat tccgttgcgg ctaaaagttt ctgccgaaga
     2461 aaaatcggca ttagaaaaag cgcagtctgc cctggcggaa ttgggtattg atttccagtc
     2521 agatgcacag catgtgacca tcagggcagt gcctttaccc ttacgccaac aaaatttaca
     2581 aatcttgatt cctgaactga taggctacct ggcgaagcag tccgtattcg aacctggcaa
     2641 tattgcgcag tggattgcac gaaatctgat gagcgaacat gcgcagtggt caatggcaca
     2701 ggccataacc ctgctggcgg acgtggaacg gttatgtccg caacttgtga aaacgccgcc
     2761 gggtggtctg ttacaatctg ttgatttaca tccggcgata aaagccctga aagatgagtg
     2821 a
//