LOCUS BBa_K1500009 4455 bp DNA linear 26-Nov-2024 DEFINITION IPTG--GFP--DnaE ACCESSION BBa_K1500009 VERSION BBa_K1500009.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..946 /label=GFP protein CDS 227..946 /label=BBa_E0040 misc_feature 1..946 /label=BBa_K1500002 misc_feature 959..962 /label=conserved RBS 955..966 /label=BBa_B0034 CDS 973..4455 /label=DnaE CDS 973..4455 /label=BBa_K1500993 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga 241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca 301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa 361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg 421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa 481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa 541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt 601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta 661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt 721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa 781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc 841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac 901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagaaagag 961 gagaaatact agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg 1021 atgatcgatg gcctggccaa aaccgcaccg ttggtaaaaa aggcggcggc gttgggtatg 1081 ccagcactgg cgatcaccga tttcaccaac ctttgtggtc tggtgaagtt ctacggagcg 1141 ggacatggcg cagggattaa gcctatcgtc ggggcagatt ttaacgtcca gtgcgacctg 1201 ctgggtgatg agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat 1261 ctgacgttgc tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc 1321 gatcgcgact ggcttatcga attaaacgaa gggttgatcc ttctttccgg cggacgcatg 1381 ggcgacgtcg gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg 1441 ttttatgaag aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg 1501 gatgaagaaa gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc 1561 gtggcgacca acgacgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc 1621 gtcgcgatcc acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg 1681 cagcaatata tgcgtagcga agaggagatg tgtgagctgt ttgccgacat ccccgaagcc 1741 cttgccaaca ccgttgagat cgccaaacgc tgtaacgtaa ccgtgcgtct tggtgaatac 1801 ttcctgccgc agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca 1861 aaagagggcc tggaagagcg tctggccttt ttattccctg atgaggaaga acgtcttaag 1921 cgccgcccgg aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc 1981 ttcccgggct acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta 2041 ccggtagggc caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc 2101 accgacctcg atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt 2161 gtctccatgc ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgag 2221 cacgtagcgg acatgtacgg tcgtgatgcg gtatcgcaga tcatcacctt cggtacaatg 2281 gcggcgaaag cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc 2341 gatcgtatct cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa 2401 gccgagccgc agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac 2461 atggcgcgca aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt 2521 atcgcgccga ccaaaattac cgattttgcg ccgctttact gcgatgaaga gggcaaacat 2581 ccggtcaccc agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc 2641 cttggtttgc gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg 2701 gcgaagaatg gcgagccgcc gctggatatc gctgcgatcc cgctggatga taagaaaagc 2761 ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg 2821 aaggacctga tcaagcgtct acaacctgac tgcttcgaag atatgatcgc cctagtggca 2881 ctgttccgcc ccggtccgtt gcaatcaggg atggtggata actttatcga ccgtaaacat 2941 ggtcgtgaag agatctccta tccggacgta cagtggcagc atgaaagcct gaaaccggta 3001 ctggagccaa cctacggcat tatcctgtat caggaacagg tcatgcagat tgcgcaggtg 3061 ctttctggtt ataccctcgg tggcgcggat atgctgcgtc gtgcgatggg taagaaaaag 3121 ccggaagaga tggctaagca acgttctgta tttgctgaag gtgcagaaaa gaacggaatc 3181 aacgctgaac tggcgatgaa aatcttcgac ctggtggaga aattcgctgg ttacggattt 3241 aacaaatcgc actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg 3301 cactatcctg cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag 3361 gtggtgggtc tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata 3421 aactccggtc tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc 3481 gcgatcaaag gggtcggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc 3541 ggctacttcc gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttgaaccgt 3601 cgcgtgctgg aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acatcgcgca 3661 gcgctgatga actcgctggg cgatgcgtta aaagcggcag atcaacacgc gaaagcggaa 3721 gctatcggtc aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa 3781 tcctacgcca gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg 3841 ttaggcctgt acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat 3901 gtcggaggcg taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct 3961 gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc 4021 tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat 4081 aaataccagc aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt 4141 gatgacttca gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc 4201 cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag 4261 cttttaaacc gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat 4321 ctctactatc agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc 4381 tctccgagcg atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa 4441 ctggagtttg actaa //