LOCUS       BBa_K1500011            1810 bp    DNA     linear       09-Dec-2025
DEFINITION  IPTG-GFP-dam
ACCESSION   BBa_K1500011
VERSION     BBa_K1500011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..946
                     /label=GFP protein
     CDS             227..946
                     /label=BBa_E0040
     misc_feature    1..946
                     /label=BBa_K1500002
     RBS             955..967
                     /label=RBS-3\Weak
     RBS             961..964
                     /label=RBS
     misc_feature    955..967
                     /label=BBa_B0032
     RBS             955..967
                     /label=BBa_B0032
     CDS             974..1810
                     /label=dam
     CDS             974..1810
                     /label=BBa_K1500994
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagtcacac
      961 aggaaagtac tagatgaaga aaaatcgcgc ttttttgaag tgggcagggg gcaagtatcc
     1021 cctgcttgat gatattaaac ggcatttgcc caagggcgaa tgtctggttg agccttttgt
     1081 aggtgccggg tcggtgtttc tcaacaccga cttttctcgt tatatccttg ccgatatcaa
     1141 tagcgacctg atcagtctct ataacattgt gaagatgcgt actgatgagt acgtacaggc
     1201 cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag gtttactatc agttccgcga
     1261 agagttcaac aaaagccagg atccgttccg tcgggcggta ctgtttttat atttgaaccg
     1321 ctacggttac aacggcctgt gtcgttacaa tctgcgcggt gagtttaacg tgccgttcgg
     1381 ccgctacaaa aaaccctatt tcccggaagc agagttgtat cacttcgctg aaaaagcgca
     1441 gaatgccttt ttctattgtg agtcttacgc cgatagcatg gcgcgcgcag atgatgcatc
     1501 cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg accgccaact ttacggcgta
     1561 tcacacaaac agttttacgc ttgaacaaca agcgcatctg gcggagatcg ccgaaggtct
     1621 ggttgagcgc catattccag tgctgatctc caatcacgat acgatgttaa cgcgtgagtg
     1681 gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc agtataagca gcaacggcgg
     1741 cacacgtaaa aaggtggacg aactgctggc tttgtacaaa ccaggagtcg tttcacccgc
     1801 gaaaaaataa
//