##gff-version 3
##sequence-region BBa_K1500015 1 1667
BBa_K1500015	.	engineered_region	1	950	.	+	0	ID=BBa_K1500001;Name=BBa_K1500001
BBa_K1500015	.	promoter	1	200	.	+	0	ID=BBa_K1500999;Name=BBa_K1500999;Parent=BBa_K1500001
BBa_K1500015	.	sequence_feature	1	200	.	+	0	ID=annotation2422932;Name=misc;Parent=BBa_K1500999
BBa_K1500015	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1500001
BBa_K1500015	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1500015	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1500015	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1500015	.	engineered_region	228	950	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_K1500001
BBa_K1500015	.	sequence_alteration	579	579	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K1500015	.	tag	936	944	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K1500015	.	CDS	228	935	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K1500015	.	ribosome_entry_site	959	971	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1500015	.	engineered_region	959	971	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1500015	.	ribosome_entry_site	965	968	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1500015	.	ribosome_entry_site	959	971	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1500015	.	CDS	978	1667	.	+	0	ID=BBa_K1500996;Name=BBa_K1500996
BBa_K1500015	.	CDS	978	1667	.	+	0	ID=annotation2423203;Name=MutH;Parent=BBa_K1500996
BBa_K1500015	.	sequence_alteration	1144	1144	.	+	0	ID=annotation2422984;Name=E56A;Parent=BBa_K1500996
>BBa_K1500015
ctttttcaaagcatagcggattgttttcaaagggagtgtaaatttatctatacagaggtaagggttgaaagcgcgacta
aattgcctgtgtaaataaaaatgtacgaaatatggattgaaaactttactttatgtgttatcgttacgtcatcctcgct
gaggatcaactatcgcaaacgagcataaacaggatcgccatctactagagtcacacaggaaagtactagatggtgagca
agggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggcca
cgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggt
ggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccg
acatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgt
ggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccc
tccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccc
tgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggc
caagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacacc
atcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaat
aatactagagtcacacaggaaagtactagatgtcccaacctcgcccactgctctctcctcccgaaactgaagaacagtt
gttagcgcaagcacagcaactttctggttatacattgggagaactggcggcacttgtcgggctggttacgccagagaat
ttaaaacgcgataaaggctggattggcgtgttactggagatctggctaggtgccagcgcagggagtaaacctgagcaag
attttgctgctctgggcgtggaacttaaaactatccctgtggatagtcttggtcgtccgctggaaacaacattcgtttg
tgttgccccgttaacgggcaatagcggggtgacctgggaaaccagccacgtgcgccacaaactcaaacgcgtactgtgg
ataccggttgaaggcgagcgcagcatcccgctggcgcagcgtcgcgttggatcaccgttgctgtggagcccgaatgaag
aggaagaccggcagctacgcgaagactgggaagaattaatggatatgattgttctcggtcaggttgagcggattaccgc
tcgtcacggggagtatttacagatacgaccgaaagcagcgaatgcgaaagcgcttaccgaagccattggtgcccggggc
gaacggattctgacgctgccacgcggcttttatttgaagaagaatttcaccagtgcactactggcccgtcattttctga
tccagtag