LOCUS       BBa_K1500016            1667 bp    DNA     linear       22-Nov-2024
DEFINITION  paroF->MCherry-MutH(E56A)
ACCESSION   BBa_K1500016
VERSION     BBa_K1500016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=misc
     promoter        1..200
                     /label=BBa_K1500999
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..935
                     /label=mCherry
     misc_feature    579..579
                     /label=C->T (removing PstI site)
     misc_feature    936..944
                     /label=LVA
     misc_feature    228..950
                     /label=BBa_J06505
     misc_feature    1..950
                     /label=BBa_K1500001
     RBS             959..971
                     /label=RBS-3\Weak
     RBS             965..968
                     /label=RBS
     misc_feature    959..971
                     /label=BBa_B0032
     RBS             959..971
                     /label=BBa_B0032
     misc_feature    1144..1144
                     /label=E56A
     CDS             978..1667
                     /label=MutH
     CDS             978..1667
                     /label=BBa_K1500996
ORIGIN
        1 ctttttcaaa gcatagcgga ttgttttcaa agggagtgta aatttatcta tacagaggta
       61 agggttgaaa gcgcgactaa attgcctgtg taaataaaaa tgtacgaaat atggattgaa
      121 aactttactt tatgtgttat cgttacgtca tcctcgctga ggatcaacta tcgcaaacga
      181 gcataaacag gatcgccatc tactagagtc acacaggaaa gtactagatg gtgagcaagg
      241 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
      301 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      361 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      421 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
      481 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      541 aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct
      601 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      661 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      721 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
      781 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
      841 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
      901 gccactccac cggcggcatg gacgagctgt acaagttagt agcttaataa tactagagtc
      961 acacaggaaa gtactagatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
     1021 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
     1081 ttgtcgggct ggttacgcca gagaatttaa aacgcgataa aggctggatt ggcgtgttac
     1141 tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg
     1201 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
     1261 tttgtgttgc cccgttaacg ggcaatagcg gggtgacctg ggaaaccagc cacgtgcgcc
     1321 acaaactcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
     1381 agcgtcgcgt tggatcaccg ttgctgtgga gcccgaatga agaggaagac cggcagctac
     1441 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggattaccg
     1501 ctcgtcacgg ggagtattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg
     1561 aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga
     1621 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtag
//