BBa_J06505
1
mCherry
monomeric RFP optimized for bacteria
2005-07-17T11:00:00Z
2015-08-31T04:08:18Z
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
Released HQ 2013
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
false
false
_20_
0
340
20
In stock
false
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
false
ytwang
annotation1585879
1
mCherry
range1585879
1
1
708
annotation1585887
1
LVA
range1585887
1
709
717
annotation1585880
1
C->T (removing PstI site)
range1585880
1
352
352
BBa_K1500996
1
BBa_K1500996
mutH (E56A)
2014-10-16T11:00:00Z
2015-05-08T01:10:47Z
E. coli (MG1655) genomic and quickchanged to E56A
mutagenized mutH useful as a mutagen protein
false
false
_1880_
0
23166
9
Not in stock
false
NA
false
Ryan Beckner
annotation2422984
1
E56A
range2422984
1
167
167
annotation2423203
1
MutH
range2423203
1
1
690
BBa_K1500999
1
BBa_K1500999
ParoF promoter
2014-10-16T11:00:00Z
2015-05-08T01:10:47Z
Cloned from wild-type DH5-alpha E. coli genomic sequence.
Used in BBa_1500001, this promoter drives expression of the AroF promoter in wild-type E. coli. Here, it is used as a tyrosine-repressible promoter sensitive to the transcription factor TyrR.
false
false
_1880_
0
23136
9
Not in stock
false
None.
false
Kevin Yang
annotation2422932
1
misc
range2422932
1
1
200
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K1500016
1
BBa_K1500016
paroF->MCherry-MutH(E56A)
2014-10-16T11:00:00Z
2015-05-08T01:10:46Z
paroF, mutH are genomic XL1Blue, mCherry is from distribution kit.
This biobrick contains mutH() and mCherry under the control of the tyrosine-sensitive promoter paroF. Unless the E. coli cell produces very high levels of tyrosine, the mutagenizing protein mutH() and mCherry will be produced. The mCherry is used as a reporter to indicate when this maximum tyrosine production is reached. mutH should cause genomic mutations that should result in the cell eventually producing high levels of tyrosine.
false
false
_1880_
0
23166
9
It's complicated
false
NA
false
Ryan Beckner
component2423363
1
BBa_B0032
component2423367
1
BBa_K1500996
component2423361
1
BBa_K1500001
annotation2423363
1
BBa_B0032
range2423363
1
959
971
annotation2423367
1
BBa_K1500996
range2423367
1
978
1667
annotation2423361
1
BBa_K1500001
range2423361
1
1
950
BBa_K1500001
1
BBa_K1500001
ParoF-mCherry backbone
2014-10-16T11:00:00Z
2015-05-08T01:10:46Z
mCherry-LVA (BBa_J06505) was used and came from the registry plasmid. ParoF was cloned from wild-type DH5-alpha E. coli genomic sequence. RBS.3 (BBa_B0032) sequence originally came from the registry and was designed directly into primer overhangs for mCherry-LVA.
A plasmid that contains the tyrosine-repressible promoter ParoF, followed by an RBS (RBS.3, medium strength) and mCherry-LVA as a reporter gene directly downstream. We used it to insert our combinations of mutator genes directly downstream of the ParoF promoter, forming the actuator module of our project which contains an optimized promoter, a reporter, and one or more mutators. The transcription factor TyrR can bind to ParoF and repress it when itself bound to tyrosine.
false
false
_1880_
0
23136
9
It's complicated
false
In order to insert mutator genes downstream of the mCherry-LVA, we would cut open the two downstream restriction sites, with corresponding cuts on the mutator genes, before ligating the insert and this plasmid together.
false
Kevin Yang
component2422931
1
BBa_J06505
component2422924
1
BBa_K1500999
component2422926
1
BBa_B0032
annotation2422931
1
BBa_J06505
range2422931
1
228
950
annotation2422926
1
BBa_B0032
range2422926
1
209
221
annotation2422924
1
BBa_K1500999
range2422924
1
1
200
BBa_K1500016_sequence
1
ctttttcaaagcatagcggattgttttcaaagggagtgtaaatttatctatacagaggtaagggttgaaagcgcgactaaattgcctgtgtaaataaaaatgtacgaaatatggattgaaaactttactttatgtgttatcgttacgtcatcctcgctgaggatcaactatcgcaaacgagcataaacaggatcgccatctactagagtcacacaggaaagtactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagtcacacaggaaagtactagatgtcccaacctcgcccactgctctctcctcccgaaactgaagaacagttgttagcgcaagcacagcaactttctggttatacattgggagaactggcggcacttgtcgggctggttacgccagagaatttaaaacgcgataaaggctggattggcgtgttactggagatctggctaggtgccagcgcagggagtaaacctgagcaagattttgctgctctgggcgtggaacttaaaactatccctgtggatagtcttggtcgtccgctggaaacaacattcgtttgtgttgccccgttaacgggcaatagcggggtgacctgggaaaccagccacgtgcgccacaaactcaaacgcgtactgtggataccggttgaaggcgagcgcagcatcccgctggcgcagcgtcgcgttggatcaccgttgctgtggagcccgaatgaagaggaagaccggcagctacgcgaagactgggaagaattaatggatatgattgttctcggtcaggttgagcggattaccgctcgtcacggggagtatttacagatacgaccgaaagcagcgaatgcgaaagcgcttaccgaagccattggtgcccggggcgaacggattctgacgctgccacgcggcttttatttgaagaagaatttcaccagtgcactactggcccgtcattttctgatccagtag
BBa_J06505_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
BBa_K1500996_sequence
1
atgtcccaacctcgcccactgctctctcctcccgaaactgaagaacagttgttagcgcaagcacagcaactttctggttatacattgggagaactggcggcacttgtcgggctggttacgccagagaatttaaaacgcgataaaggctggattggcgtgttactggagatctggctaggtgccagcgcagggagtaaacctgagcaagattttgctgctctgggcgtggaacttaaaactatccctgtggatagtcttggtcgtccgctggaaacaacattcgtttgtgttgccccgttaacgggcaatagcggggtgacctgggaaaccagccacgtgcgccacaaactcaaacgcgtactgtggataccggttgaaggcgagcgcagcatcccgctggcgcagcgtcgcgttggatcaccgttgctgtggagcccgaatgaagaggaagaccggcagctacgcgaagactgggaagaattaatggatatgattgttctcggtcaggttgagcggattaccgctcgtcacggggagtatttacagatacgaccgaaagcagcgaatgcgaaagcgcttaccgaagccattggtgcccggggcgaacggattctgacgctgccacgcggcttttatttgaagaagaatttcaccagtgcactactggcccgtcattttctgatccagtag
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1500001_sequence
1
ctttttcaaagcatagcggattgttttcaaagggagtgtaaatttatctatacagaggtaagggttgaaagcgcgactaaattgcctgtgtaaataaaaatgtacgaaatatggattgaaaactttactttatgtgttatcgttacgtcatcctcgctgaggatcaactatcgcaaacgagcataaacaggatcgccatctactagagtcacacaggaaagtactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
BBa_K1500999_sequence
1
ctttttcaaagcatagcggattgttttcaaagggagtgtaaatttatctatacagaggtaagggttgaaagcgcgactaaattgcctgtgtaaataaaaatgtacgaaatatggattgaaaactttactttatgtgttatcgttacgtcatcctcgctgaggatcaactatcgcaaacgagcataaacaggatcgccatc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z