Lambda cI generator consisting of a strong constitutive promotor (BBa_J23101), a strong ribosome binding site (BBa_B0034), a lambda cI coding sequence (BBa_K150001) and a double terminator (BBa_B0015). The lambda cI coding sequence was optimized for high expression levels in E. coli
Christian Moritz, Maximilian Hoerner, Anna Stoeckl, Yin Cai, Stephen Kraemer
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Transcriptional terminator consisting of a 64 bp stem-loop.
aminoacid sequence from: NP_040628 and NC_001416
Coding region for the cI repressor based on cI repressor aminoacid sequence from bacteriophage lambda. The nucleotide sequence was optimized for high expression levels in E. coli using Geneart's GeneOptimizer technology.
Conversion of the iGEM parts registry to SBOL2.1