BBa_K150004
type: ComponentDefinition
persistent identity: BBa K150004
display id: BBa_K150004
version: 1
was generated by: iGEM to SBOL conversion
description: lambda cI generator
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Lambda cI generator consisting of a strong constitutive promotor (BBa_J23101), a strong ribosome binding site (BBa_B0034), a lambda cI coding sequence (BBa_K150001) and a double terminator (BBa_B0015). The lambda cI coding sequence was optimized for high expression levels in E. coli
discontinued: false
dominant: false
experience: experience/Works
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz, Maximilian Hoerner, Anna Stoeckl, Yin Cai, Stephen Kraemer
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23101
component: BBa_B0032
component: BBa_K150103
component: BBa_K150001
component: BBa_K150102
component: BBa_B0010
component: BBa_K150101
component: BBa_B0012
component: BBa_K150100
sequence annotation: BBa_J23101
sequence annotation: BBa_K150103
sequence annotation: BBa_B0032
sequence annotation: BBa_K150102
sequence annotation: BBa_K150001
sequence annotation: BBa_K150101
sequence annotation: BBa_B0010
sequence annotation: BBa_K150100
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K150004_sequence/1

BBa_J23101
type: Component
persistent identity: component1983332
display id: component1983332
version: 1
top level: BBa_K150004
definition: BBa_J23101
access: public

BBa_B0032
type: Component
persistent identity: component1983335
display id: component1983335
version: 1
top level: BBa_K150004
definition: BBa_B0032
access: public

BBa_K150103
type: Component
persistent identity: component1983333
display id: component1983333
version: 1
top level: BBa_K150004
definition: BBa_K150103
access: public

BBa_K150001
type: Component
persistent identity: component1983339
display id: component1983339
version: 1
top level: BBa_K150004
definition: BBa_K150001
access: public

BBa_K150102
type: Component
persistent identity: component1983337
display id: component1983337
version: 1
top level: BBa_K150004
definition: BBa_K150102
access: public

BBa_B0010
type: Component
persistent identity: component1983341
display id: component1983341
version: 1
top level: BBa_K150004
definition: BBa_B0010
access: public

BBa_K150101
type: Component
persistent identity: component1983340
display id: component1983340
version: 1
top level: BBa_K150004
definition: BBa_K150101
access: public

BBa_B0012
type: Component
persistent identity: component1983344
display id: component1983344
version: 1
top level: BBa_K150004
definition: BBa_B0012
access: public

BBa_K150100
type: Component
persistent identity: component1983343
display id: component1983343
version: 1
top level: BBa_K150004
definition: BBa_K150100
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation1983332
display id: annotation1983332
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983332/range1983332/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K150004/annotation1983332/range1983332/1
type: Range
persistent identity: range1983332
display id: range1983332
version: 1
top level: BBa_K150004
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K150103
type: SequenceAnnotation
persistent identity: annotation1983333
display id: annotation1983333
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983333/range1983333/1
component: BBa_K150103

https://synbiohub.org/public/igem/BBa_K150004/annotation1983333/range1983333/1
type: Range
persistent identity: range1983333
display id: range1983333
version: 1
top level: BBa_K150004
direction: direction/reverse
start: 36
end: 43
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1983335
display id: annotation1983335
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983335/range1983335/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K150004/annotation1983335/range1983335/1
type: Range
persistent identity: range1983335
display id: range1983335
version: 1
top level: BBa_K150004
direction: direction/forward
start: 44
end: 56
orientation: inline

BBa_K150102
type: SequenceAnnotation
persistent identity: annotation1983337
display id: annotation1983337
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983337/range1983337/1
component: BBa_K150102

https://synbiohub.org/public/igem/BBa_K150004/annotation1983337/range1983337/1
type: Range
persistent identity: range1983337
display id: range1983337
version: 1
top level: BBa_K150004
direction: direction/reverse
start: 57
end: 62
orientation: inline

BBa_K150001
type: SequenceAnnotation
persistent identity: annotation1983339
display id: annotation1983339
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983339/range1983339/1
component: BBa_K150001

https://synbiohub.org/public/igem/BBa_K150004/annotation1983339/range1983339/1
type: Range
persistent identity: range1983339
display id: range1983339
version: 1
top level: BBa_K150004
direction: direction/reverse
start: 63
end: 776
orientation: inline

BBa_K150101
type: SequenceAnnotation
persistent identity: annotation1983340
display id: annotation1983340
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983340/range1983340/1
component: BBa_K150101

https://synbiohub.org/public/igem/BBa_K150004/annotation1983340/range1983340/1
type: Range
persistent identity: range1983340
display id: range1983340
version: 1
top level: BBa_K150004
direction: direction/reverse
start: 777
end: 784
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1983341
display id: annotation1983341
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983341/range1983341/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K150004/annotation1983341/range1983341/1
type: Range
persistent identity: range1983341
display id: range1983341
version: 1
top level: BBa_K150004
direction: direction/forward
start: 785
end: 864
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation1983343
display id: annotation1983343
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983343/range1983343/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150004/annotation1983343/range1983343/1
type: Range
persistent identity: range1983343
display id: range1983343
version: 1
top level: BBa_K150004
direction: direction/reverse
start: 865
end: 872
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1983344
display id: annotation1983344
version: 1
top level: BBa_K150004
location: https://synbiohub.org/public/igem/BBa_K150004/annotation1983344/range1983344/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K150004/annotation1983344/range1983344/1
type: Range
persistent identity: range1983344
display id: range1983344
version: 1
top level: BBa_K150004
direction: direction/forward
start: 873
end: 913
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K150001
type: ComponentDefinition
persistent identity: BBa K150001
display id: BBa_K150001
version: 1
was generated by: iGEM to SBOL conversion
description: lambda cI
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: aminoacid sequence from: NP_040628 and NC_001416
owned by: james
owned by: myers
mutable description: Coding region for the cI repressor based on cI repressor aminoacid sequence from bacteriophage lambda. The nucleotide sequence was optimized for high expression levels in E. coli using Geneart's GeneOptimizer technology.
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: lambda cI
sequence: https://synbiohub.org/public/igem/BBa_K150001_sequence/1

lambda cI
type: SequenceAnnotation
persistent identity: annotation1983143
display id: annotation1983143
version: 1
top level: BBa_K150001
location: https://synbiohub.org/public/igem/BBa_K150001/annotation1983143/range1983143/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K150001/annotation1983143/range1983143/1
type: Range
persistent identity: range1983143
display id: range1983143
version: 1
top level: BBa_K150001
direction: direction/forward
start: 1
end: 714
orientation: inline

BBa_K150100
type: ComponentDefinition
persistent identity: BBa K150100
display id: BBa_K150100
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD00
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: original scar from BioBricks
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150100_sequence/1

BBa_K150101
type: ComponentDefinition
persistent identity: BBa K150101
display id: BBa_K150101
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD01
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: linker sequence used in BBa_K15004
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K150101_sequence/1

BBa_K150102
type: ComponentDefinition
persistent identity: BBa K150102
display id: BBa_K150102
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD02
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: linker sequence used in BBa_K150004
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K150102_sequence/1

BBa_K150103
type: ComponentDefinition
persistent identity: BBa K150103
display id: BBa_K150103
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD03
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: linker sequence used in BBa_K150004
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K150103_sequence/1

https://synbiohub.org/public/igem/BBa_K150004_sequence/1
type: Sequence
persistent identity: BBa K150004 sequence
display id: BBa_K150004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctagagctctcacacaggaaagggtaccatgagcaccaaaaaaaaaccgctgacccaagaacagctggaagatgcgcgtcgtctgaaagcgatctacgaaaaaaaaaaaaacgaactgggcctgagccaggaaagcgttgcggataaaatgggcatgggccagagcggtgtgggtgcgctgtttaacggcattaacgcgctgaacgcgtataacgctgctctgctggccaaaattctgaaagtgagcgtggaagaatttagcccgagcattgcacgcgaaatctacgaaatgtatgaagcggtgagcatgcagccgagcctgcgtagcgaatatgaatatccggtgtttagccatgtgcaggcaggcatgttttctccggaactgcgtacctttaccaaaggtgatgcggaacgttgggtgagcaccaccaaaaaagcgagcgatagcgcgttttggctggaagtggaaggcaacagcatgaccgcaccgaccggcagcaaaccgagctttccggatggcatgctgattctggtggatccggaacaggcggtggaaccgggtgatttttgcattgcgcgtctgggtggtgatgagttcaccttcaaaaaactgattcgtgatagcggtcaggtgtttctgcaaccgctgaatccgcagtatccgatgattccgtgcaacgaaagctgtagcgtggtgggcaaagtgattgcgagccagtggccggaagaaacctttggctaagagctcagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150001_sequence/1
type: Sequence
persistent identity: BBa K150001 sequence
display id: BBa_K150001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaccaaaaaaaaaccgctgacccaagaacagctggaagatgcgcgtcgtctgaaagcgatctacgaaaaaaaaaaaaacgaactgggcctgagccaggaaagcgttgcggataaaatgggcatgggccagagcggtgtgggtgcgctgtttaacggcattaacgcgctgaacgcgtataacgctgctctgctggccaaaattctgaaagtgagcgtggaagaatttagcccgagcattgcacgcgaaatctacgaaatgtatgaagcggtgagcatgcagccgagcctgcgtagcgaatatgaatatccggtgtttagccatgtgcaggcaggcatgttttctccggaactgcgtacctttaccaaaggtgatgcggaacgttgggtgagcaccaccaaaaaagcgagcgatagcgcgttttggctggaagtggaaggcaacagcatgaccgcaccgaccggcagcaaaccgagctttccggatggcatgctgattctggtggatccggaacaggcggtggaaccgggtgatttttgcattgcgcgtctgggtggtgatgagttcaccttcaaaaaactgattcgtgatagcggtcaggtgtttctgcaaccgctgaatccgcagtatccgatgattccgtgcaacgaaagctgtagcgtggtgggcaaagtgattgcgagccagtggccggaagaaacctttggctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150100_sequence/1
type: Sequence
persistent identity: BBa K150100 sequence
display id: BBa_K150100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150101_sequence/1
type: Sequence
persistent identity: BBa K150101 sequence
display id: BBa_K150101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagctcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150102_sequence/1
type: Sequence
persistent identity: BBa K150102 sequence
display id: BBa_K150102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150103_sequence/1
type: Sequence
persistent identity: BBa K150103 sequence
display id: BBa_K150103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagagctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z