BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2045 1 LuxR/HSL range2045 1 1 20 annotation2047 1 -10 range2047 1 42 47 annotation2048 1 start range2048 1 53 53 annotation2046 1 -35 range2046 1 20 25 annotation7070 1 BBa_R0062 range7070 1 1 55 BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation1765 1 A range1765 1 492 492 annotation2213986 1 Help:Barcodes range2213986 1 757 781 annotation7039 1 BBa_C0062 range7039 1 1 756 annotation1766 1 luxR range1766 1 1 750 annotation1764 1 T range1764 1 174 174 annotation1762 1 prefix range1762 1 1 2 BBa_K150005 1 BBa_K150005 ribosome binding site of pTrc99a 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z - ribosome binding site of pTrc99a false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer annotation1983190 1 BBa_K150005 range1983190 1 1 13 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 BBa_K150104 1 BBa_K150104 linkerHD04 2008-10-20T11:00:00Z 2015-05-08T01:10:47Z - DNA linker region between lux pR promoter BBa_R0062 and ribosome binding site pTrc99a (BBa_) for the ColicinE1 Producer Controlled by 3OC6HSL Receiver Device (BBa_). false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer annotation1983191 1 linkerHD04 range1983191 1 1 8 BBa_K150008 1 BamHI BamHI restriction enzyme site 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z - BamHI restriction site false true _234_ 0 3299 9 Not in stock false - false Pascal Kraemer BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K150009 1 BBa_K150009 ColicinE1 Producer Controlled by 3OC6HSL Receiver Device 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z - The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE1 production. This activation of the lux pR promoter can additionally lead to expression of the kil protein by read through of the terminator t1 and therefore to lysis of the cell and colcin release. The colicinE1 immunity protein (imm) lying on the reverse strand is expressend under a constitutive promotor. false false _234_ 0 3299 9 It's complicated false - false Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne component2305761 1 BBa_K150007 component2305735 1 BBa_C0062 component2305739 1 BBa_K150100 component2305749 1 BBa_R0062 component2305757 1 BBa_K150008 component2305724 1 BBa_R0040 component2305743 1 BBa_B0012 component2305740 1 BBa_B0010 component2305756 1 BBa_K150005 component2305747 1 BBa_K150100 component2305731 1 BBa_B0034 component2305732 1 BBa_K150107 component2305729 1 BBa_K150100 component2305754 1 BBa_K150104 component2305742 1 BBa_K150100 annotation2305757 1 BBa_K150008 range2305757 1 1083 1088 annotation2305739 1 BBa_K150100 range2305739 1 862 869 annotation2305749 1 BBa_R0062 range2305749 1 1007 1061 annotation2305740 1 BBa_B0010 range2305740 1 870 949 annotation2305732 1 BBa_K150107 range2305732 1 75 80 annotation2305743 1 BBa_B0012 range2305743 1 958 998 annotation2305761 1 BBa_K150007 range2305761 1 1089 3180 annotation2305754 1 BBa_K150104 range2305754 1 1062 1069 annotation2305742 1 BBa_K150100 range2305742 1 950 957 annotation2305756 1 BBa_K150005 range2305756 1 1070 1082 annotation2305735 1 BBa_C0062 range2305735 1 81 836 annotation2305729 1 BBa_K150100 range2305729 1 55 62 annotation2305731 1 BBa_B0034 range2305731 1 63 74 annotation2305747 1 BBa_K150100 range2305747 1 999 1006 annotation2305724 1 BBa_R0040 range2305724 1 1 54 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K150007 1 BBa_K150007 ColicinE1 operon 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z This colicinE1 cassette is derived from the naturally existing colicinE1 plasmid out of the E. coli strain JC411. This colicinE1 operon contains all three important genes for colicinE1 expression and release. The colE1 gene codes for the lethal colicinE1 protein. The gene for the colE1 immunity protein located on the reverse strand is responsible for the immunity of the producing host bacteria to the colicinE1 and is therefore controlled by a constitutive promoter. Expression of the kil protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE1. false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer annotation1983359 1 colE1 immunity protein range1983359 1 1566 1907 annotation1983358 1 colE1 protein range1983358 1 1 1569 annotation1983360 1 kil protein range1983360 1 1955 2092 BBa_K150100 1 BBa_K150100 linkerHD00 2008-10-20T11:00:00Z 2015-05-08T01:10:47Z - original scar from BioBricks false false _234_ 0 3314 9 Not in stock false - false Christian Moritz BBa_K150107 1 BBa_K150107 linkerHD07 2008-10-20T11:00:00Z 2015-05-08T01:10:47Z - linkerHD07 false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K150007_sequence 1 atggaaaccgcggtagcgtactataaagatggtgttccttatgatgataagggacaggtaattattactcttttgaatggtactcctgacgggagtggctctggcggcggaggtggaaaaggaggcagtaaaagtgaaagttcagcagctattcatgcaactgctaaatggtctactgctcaattaaagaaaacacaggcagagcaggctgcccgggcaaaagcagcagcggaagcacaggcgaaagcaaaggcaaacagggatgcgctgactcagcgcctgaaggatatcgtgaatgaggctcttcgtcacaatgcctcacgtacgccttcagcaacagagcttgctcatgctaataatgcagctatgcaggcggaagcagagcgtttgcgccttgcgaaagcagaagaaaaagcccgtaaagaagcggaagcagcagaaaaggcttttcaggaagcagaacaacgacgtaaagagattgaacgggagaaggctgaaacagaacgccagttgaaactggctgaagctgaagagaaacgactggctgcattgagtgaagaagctaaagctgttgagatcgcccaaaaaaaactttctgctgcacaatctgaagtggtgaaaatggatggagagattaagactctcaattctcgtttaagctccagtatccatgcccgtgatgcagaaatgaaaacgctcgctggaaaacgaaatgaactggctcaggcatccgctaaatataaagaactggatgagctggtcaaaaaactatcaccaagagccaatgatccgcttcagaaccgtcctttttttgaagcaaccagacgacgggttggggccggtaagattagagaagaaaaacaaaaacaggtaacagcatcagaaacacgtattaaccggataaatgctgatataactcagatccagaaggctatttctcaggtcagtaataatcgtaatgccggtatcgctcgtgttcatgaagctgaagaaaatttgaaaaaagcacagaataatctccttaattcacagattaaggatgctgttgatgcaacagttagcttttatcaaacgctgactgaaaaatatggtgaaaaatattcgaaaatggcacaggaacttgctgataagtctaaaggtaagaaaatcggcaatgtgaatgaagctctcgctgcttttgaaaaatacaaggatgttttaaataagaaattcagcaaagccgatcgtgatgctatttttaatgcgttggcatcggtgaagtatgatgactgggctaaacatttagatcagtttgccaagtacttgaagattacggggcatgtttcttttggatatgatgtggtatctgatatcctaaaaattaaggatacaggtgactggaagccactatttcttacattagagaagaaagcagcagatgcaggggtgagttatgttgttgctttactttttagcttgcttgctggaactacattaggtatttggggtattgctattgttacaggtattctatgctcctatattgataagaataaacttaatactataaatgaggtgttagggatttaattattctttattgatataaaaagtcctagcaatccaaatgggattgctaggaccaaacaaagtagattatatagcataaataggtttaattttgctacgggggcgttatttaggttttttcttctttcgaaaaaatctttctttatgaagttaaaagctatgtattcaatagcatattttgaatatggacatagaatagtgcttatcactattgcatatagcatcttatctgacacaaggaaataatacccttcgctgttttttgttataaggtatatatatataagtgtgcagtacaggccaaataaaatattttttatgtagtatcttaagctcataaattaaacctcgccatatattcttttcattttataaggatcgagttatgaggaaaagattttttgtgggaatattcgcgataaacctccttgttggatgtcaggctaactatatacgtgatgttcagggagggaccatcgcaccatcctcctcttctaaactgacggggatcgcggttcagtgg BBa_K150107_sequence 1 tactag BBa_K150009_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaggaaacagaaccggatccatggaaaccgcggtagcgtactataaagatggtgttccttatgatgataagggacaggtaattattactcttttgaatggtactcctgacgggagtggctctggcggcggaggtggaaaaggaggcagtaaaagtgaaagttcagcagctattcatgcaactgctaaatggtctactgctcaattaaagaaaacacaggcagagcaggctgcccgggcaaaagcagcagcggaagcacaggcgaaagcaaaggcaaacagggatgcgctgactcagcgcctgaaggatatcgtgaatgaggctcttcgtcacaatgcctcacgtacgccttcagcaacagagcttgctcatgctaataatgcagctatgcaggcggaagcagagcgtttgcgccttgcgaaagcagaagaaaaagcccgtaaagaagcggaagcagcagaaaaggcttttcaggaagcagaacaacgacgtaaagagattgaacgggagaaggctgaaacagaacgccagttgaaactggctgaagctgaagagaaacgactggctgcattgagtgaagaagctaaagctgttgagatcgcccaaaaaaaactttctgctgcacaatctgaagtggtgaaaatggatggagagattaagactctcaattctcgtttaagctccagtatccatgcccgtgatgcagaaatgaaaacgctcgctggaaaacgaaatgaactggctcaggcatccgctaaatataaagaactggatgagctggtcaaaaaactatcaccaagagccaatgatccgcttcagaaccgtcctttttttgaagcaaccagacgacgggttggggccggtaagattagagaagaaaaacaaaaacaggtaacagcatcagaaacacgtattaaccggataaatgctgatataactcagatccagaaggctatttctcaggtcagtaataatcgtaatgccggtatcgctcgtgttcatgaagctgaagaaaatttgaaaaaagcacagaataatctccttaattcacagattaaggatgctgttgatgcaacagttagcttttatcaaacgctgactgaaaaatatggtgaaaaatattcgaaaatggcacaggaacttgctgataagtctaaaggtaagaaaatcggcaatgtgaatgaagctctcgctgcttttgaaaaatacaaggatgttttaaataagaaattcagcaaagccgatcgtgatgctatttttaatgcgttggcatcggtgaagtatgatgactgggctaaacatttagatcagtttgccaagtacttgaagattacggggcatgtttcttttggatatgatgtggtatctgatatcctaaaaattaaggatacaggtgactggaagccactatttcttacattagagaagaaagcagcagatgcaggggtgagttatgttgttgctttactttttagcttgcttgctggaactacattaggtatttggggtattgctattgttacaggtattctatgctcctatattgataagaataaacttaatactataaatgaggtgttagggatttaattattctttattgatataaaaagtcctagcaatccaaatgggattgctaggaccaaacaaagtagattatatagcataaataggtttaattttgctacgggggcgttatttaggttttttcttctttcgaaaaaatctttctttatgaagttaaaagctatgtattcaatagcatattttgaatatggacatagaatagtgcttatcactattgcatatagcatcttatctgacacaaggaaataatacccttcgctgttttttgttataaggtatatatatataagtgtgcagtacaggccaaataaaatattttttatgtagtatcttaagctcataaattaaacctcgccatatattcttttcattttataaggatcgagttatgaggaaaagattttttgtgggaatattcgcgataaacctccttgttggatgtcaggctaactatatacgtgatgttcagggagggaccatcgcaccatcctcctcttctaaactgacggggatcgcggttcagtgg BBa_K150005_sequence 1 aggaaacagaacc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K150104_sequence 1 tactagag BBa_K150100_sequence 1 tactagag BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K150008_sequence 1 ggatcc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z