##gff-version 3
##sequence-region BBa_K150013 1 2112
BBa_K150013	.	ribosome_entry_site	1070	1082	.	+	0	ID=BBa_K150005;Name=BBa_K150005
BBa_K150013	.	ribosome_entry_site	1070	1082	.	+	0	ID=annotation1983190;Name=BBa_K150005;Parent=BBa_K150005
BBa_K150013	.	sequence_feature	55	62	.	+	0	ID=BBa_K150100;Name=BBa_K150100
BBa_K150013	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K150013	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K150013	.	sequence_feature	999	1006	.	+	0	ID=BBa_K150100;Name=BBa_K150100
BBa_K150013	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K150013	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K150013	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K150013	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K150013	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K150013	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K150013	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K150013	.	sequence_feature	75	80	.	+	0	ID=BBa_K150107;Name=BBa_K150107
BBa_K150013	.	sequence_feature	1062	1069	.	+	0	ID=BBa_K150104;Name=BBa_K150104
BBa_K150013	.	sequence_feature	1062	1069	.	+	0	ID=annotation1983191;Name=linkerHD04;Parent=BBa_K150104
BBa_K150013	.	sequence_feature	950	957	.	+	0	ID=BBa_K150100;Name=BBa_K150100
BBa_K150013	.	sequence_feature	1083	1088	.	+	0	ID=BBa_K150008;Name=BBa_K150008
BBa_K150013	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K150013	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K150013	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K150013	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K150013	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K150013	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K150013	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K150013	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K150013	.	sequence_feature	862	869	.	+	0	ID=BBa_K150100;Name=BBa_K150100
BBa_K150013	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K150013	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K150013	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K150013	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K150013	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K150013	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K150013	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K150013	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K150013	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K150013	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K150013	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K150013	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K150013	.	engineered_region	1089	2112	.	+	0	ID=BBa_K150012;Name=BBa_K150012
BBa_K150013	.	CDS	1089	1708	.	+	0	ID=annotation1983740;Name=colE9 protein;Parent=BBa_K150012
BBa_K150013	.	CDS	2032	2112	.	+	0	ID=annotation1983742;Name=atypical lysis protein;Parent=BBa_K150012
BBa_K150013	.	CDS	1710	1970	.	+	0	ID=annotation1983741;Name=colE9 immunity protein;Parent=BBa_K150012
>BBa_K150013
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaggaaacagaaccggatccatcgatttgcccatgacc
caatggctggcggtcacagaatgtggcaaatggccgggcttaaagctcagcgggcgcagacggatgtaaataataagca
ggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacag
aaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggta
aaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctga
taagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgag
cttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctccgtttactccaaagaatcaacagg
tcggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatat
ccgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaaaatggaactgaagcatagcattagtgatta
tacagaagctgaatttttacagcttgtaacaacaatttgtaatgcggacacttccagtgaagaagaactggttaaattg
gttacacactttgaggaaatgactgagcaccctagtggtagtgatttaatatattacccaaaagaaggtgatgatgact
caccttcaggtattgtaaacacagtaaaacaatggcgagccgctaacggtaagtcaggatttaaacagggctaaaatat
gagtgccggttgtttaaggatgaatggctggcattctttcacaacaaggagtcgttatgaaaaaaataacagggattat
tttattgcttcttgcagtcattattctgtctgcatggggttctaagccgaaaacctag