BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2213986 1 Help:Barcodes range2213986 1 757 781 annotation7039 1 BBa_C0062 range7039 1 1 756 annotation1762 1 prefix range1762 1 1 2 annotation1764 1 T range1764 1 174 174 annotation1766 1 luxR range1766 1 1 750 annotation1765 1 A range1765 1 492 492 BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2048 1 start range2048 1 53 53 annotation2047 1 -10 range2047 1 42 47 annotation2046 1 -35 range2046 1 20 25 annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2045 1 LuxR/HSL range2045 1 1 20 BBa_K150104 1 BBa_K150104 linkerHD04 2008-10-20T11:00:00Z 2015-05-08T01:10:47Z - DNA linker region between lux pR promoter BBa_R0062 and ribosome binding site pTrc99a (BBa_) for the ColicinE1 Producer Controlled by 3OC6HSL Receiver Device (BBa_). false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer annotation1983191 1 linkerHD04 range1983191 1 1 8 BBa_K150008 1 BamHI BamHI restriction enzyme site 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z - BamHI restriction site false true _234_ 0 3299 9 Not in stock false - false Pascal Kraemer BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K150013 1 BBa_K150013 ColicinE9 Producer Controlled by 3OC6HSL Receiver Device (short) 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z - The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE9 production. This activation of the lux pR promoter can additionally lead to expression of the atypical lysis protein by read through of the terminator behind the colE9 gene and therefore to lysis of the cell and colcin release. The colicinE9 immunity protein located on the reverse strand is expressed under a constitutive promoter. false false _234_ 0 3299 9 It's complicated false - false Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne component2305799 1 BBa_K150012 component2305762 1 BBa_R0040 component2305778 1 BBa_B0010 component2305767 1 BBa_K150100 component2305792 1 BBa_K150104 component2305773 1 BBa_C0062 component2305780 1 BBa_K150100 component2305795 1 BBa_K150008 component2305769 1 BBa_B0034 component2305787 1 BBa_R0062 component2305781 1 BBa_B0012 component2305770 1 BBa_K150107 component2305794 1 BBa_K150005 component2305777 1 BBa_K150100 component2305785 1 BBa_K150100 annotation2305767 1 BBa_K150100 range2305767 1 55 62 annotation2305773 1 BBa_C0062 range2305773 1 81 836 annotation2305799 1 BBa_K150012 range2305799 1 1089 2112 annotation2305781 1 BBa_B0012 range2305781 1 958 998 annotation2305794 1 BBa_K150005 range2305794 1 1070 1082 annotation2305780 1 BBa_K150100 range2305780 1 950 957 annotation2305787 1 BBa_R0062 range2305787 1 1007 1061 annotation2305777 1 BBa_K150100 range2305777 1 862 869 annotation2305792 1 BBa_K150104 range2305792 1 1062 1069 annotation2305795 1 BBa_K150008 range2305795 1 1083 1088 annotation2305762 1 BBa_R0040 range2305762 1 1 54 annotation2305769 1 BBa_B0034 range2305769 1 63 74 annotation2305785 1 BBa_K150100 range2305785 1 999 1006 annotation2305778 1 BBa_B0010 range2305778 1 870 949 annotation2305770 1 BBa_K150107 range2305770 1 75 80 BBa_K150005 1 BBa_K150005 ribosome binding site of pTrc99a 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z - ribosome binding site of pTrc99a false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer annotation1983190 1 BBa_K150005 range1983190 1 1 13 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K150012 1 BBa_K150012 colicinE9 operon (short) 2008-10-20T11:00:00Z 2015-05-08T01:10:46Z - This colicinE9 operon (short) contains all three important genes for colicinE9 expression and release. The colE9 gene codes for the lethal colicinE9 protein. The gene for the colE9 immunity protein is responsible for the immunity of the producing host bacteria to the colicinE9 and is therefore controlled by a constitutive promoter. Expression of the atypical lysis protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE9. false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer annotation1983740 1 colE9 protein range1983740 1 1 620 annotation1983742 1 atypical lysis protein range1983742 1 944 1024 annotation1983741 1 colE9 immunity protein range1983741 1 622 882 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K150100 1 BBa_K150100 linkerHD00 2008-10-20T11:00:00Z 2015-05-08T01:10:47Z - original scar from BioBricks false false _234_ 0 3314 9 Not in stock false - false Christian Moritz BBa_K150107 1 BBa_K150107 linkerHD07 2008-10-20T11:00:00Z 2015-05-08T01:10:47Z - linkerHD07 false false _234_ 0 3299 9 Not in stock false - false Pascal Kraemer BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K150107_sequence 1 tactag BBa_K150013_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaggaaacagaaccggatccatcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccgggcttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgagcttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctccgtttactccaaagaatcaacaggtcggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatatccgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaaaatggaactgaagcatagcattagtgattatacagaagctgaatttttacagcttgtaacaacaatttgtaatgcggacacttccagtgaagaagaactggttaaattggttacacactttgaggaaatgactgagcaccctagtggtagtgatttaatatattacccaaaagaaggtgatgatgactcaccttcaggtattgtaaacacagtaaaacaatggcgagccgctaacggtaagtcaggatttaaacagggctaaaatatgagtgccggttgtttaaggatgaatggctggcattctttcacaacaaggagtcgttatgaaaaaaataacagggattattttattgcttcttgcagtcattattctgtctgcatggggttctaagccgaaaacctag BBa_K150012_sequence 1 atcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccgggcttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgagcttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctccgtttactccaaagaatcaacaggtcggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatatccgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaaaatggaactgaagcatagcattagtgattatacagaagctgaatttttacagcttgtaacaacaatttgtaatgcggacacttccagtgaagaagaactggttaaattggttacacactttgaggaaatgactgagcaccctagtggtagtgatttaatatattacccaaaagaaggtgatgatgactcaccttcaggtattgtaaacacagtaaaacaatggcgagccgctaacggtaagtcaggatttaaacagggctaaaatatgagtgccggttgtttaaggatgaatggctggcattctttcacaacaaggagtcgttatgaaaaaaataacagggattattttattgcttcttgcagtcattattctgtctgcatggggttctaagccgaaaacctag BBa_K150005_sequence 1 aggaaacagaacc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K150104_sequence 1 tactagag BBa_K150100_sequence 1 tactagag BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K150008_sequence 1 ggatcc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z