BBa_K150013
type: ComponentDefinition
persistent identity: BBa K150013
display id: BBa_K150013
version: 1
was generated by: iGEM to SBOL conversion
description: ColicinE9 Producer Controlled by 3OC6HSL Receiver Device (short)
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE9 production. This activation of the lux pR promoter can additionally lead to expression of the atypical lysis protein by read through of the terminator behind the colE9 gene and therefore to lysis of the cell and colcin release. The colicinE9 immunity protein located on the reverse strand is expressed under a constitutive promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K150012
component: BBa_K150104
component: BBa_R0062
component: BBa_K150008
component: BBa_K150005
component: BBa_K150100
component: BBa_B0010
component: BBa_B0012
component: BBa_K150107
component: BBa_B0034
component: BBa_C0062
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_K150100
sequence annotation: BBa_B0034
sequence annotation: BBa_K150107
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_K150104
sequence annotation: BBa_K150005
sequence annotation: BBa_K150008
sequence annotation: BBa_K150012
sequence: https://synbiohub.org/public/igem/BBa_K150013_sequence/1

BBa_K150012
type: Component
persistent identity: component2305799
display id: component2305799
version: 1
top level: BBa_K150013
definition: BBa_K150012
access: public

BBa_K150104
type: Component
persistent identity: component2305792
display id: component2305792
version: 1
top level: BBa_K150013
definition: BBa_K150104
access: public

BBa_R0062
type: Component
persistent identity: component2305787
display id: component2305787
version: 1
top level: BBa_K150013
definition: lux pR
access: public

BBa_K150008
type: Component
persistent identity: component2305795
display id: component2305795
version: 1
top level: BBa_K150013
definition: BamHI
access: public

BBa_K150005
type: Component
persistent identity: component2305794
display id: component2305794
version: 1
top level: BBa_K150013
definition: BBa_K150005
access: public

BBa_K150100
type: Component
persistent identity: component2305780
display id: component2305780
version: 1
top level: BBa_K150013
definition: BBa_K150100
access: public

BBa_B0010
type: Component
persistent identity: component2305778
display id: component2305778
version: 1
top level: BBa_K150013
definition: BBa_B0010
access: public

BBa_K150100
type: Component
persistent identity: component2305785
display id: component2305785
version: 1
top level: BBa_K150013
definition: BBa_K150100
access: public

BBa_B0012
type: Component
persistent identity: component2305781
display id: component2305781
version: 1
top level: BBa_K150013
definition: BBa_B0012
access: public

BBa_K150107
type: Component
persistent identity: component2305770
display id: component2305770
version: 1
top level: BBa_K150013
definition: BBa_K150107
access: public

BBa_B0034
type: Component
persistent identity: component2305769
display id: component2305769
version: 1
top level: BBa_K150013
definition: BBa_B0034
access: public

BBa_K150100
type: Component
persistent identity: component2305777
display id: component2305777
version: 1
top level: BBa_K150013
definition: BBa_K150100
access: public

BBa_C0062
type: Component
persistent identity: component2305773
display id: component2305773
version: 1
top level: BBa_K150013
definition: luxr
access: public

BBa_K150100
type: Component
persistent identity: component2305767
display id: component2305767
version: 1
top level: BBa_K150013
definition: BBa_K150100
access: public

BBa_R0040
type: Component
persistent identity: component2305762
display id: component2305762
version: 1
top level: BBa_K150013
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2305762
display id: annotation2305762
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305762/range2305762/1
component: BBa_R0040

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type: Range
persistent identity: range2305762
display id: range2305762
version: 1
top level: BBa_K150013
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305767
display id: annotation2305767
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305767/range2305767/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150013/annotation2305767/range2305767/1
type: Range
persistent identity: range2305767
display id: range2305767
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 55
end: 62
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2305769
display id: annotation2305769
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305769/range2305769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K150013/annotation2305769/range2305769/1
type: Range
persistent identity: range2305769
display id: range2305769
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K150107
type: SequenceAnnotation
persistent identity: annotation2305770
display id: annotation2305770
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305770/range2305770/1
component: BBa_K150107

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type: Range
persistent identity: range2305770
display id: range2305770
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 75
end: 80
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2305773
display id: annotation2305773
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305773/range2305773/1
component: BBa_C0062

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type: Range
persistent identity: range2305773
display id: range2305773
version: 1
top level: BBa_K150013
direction: direction/forward
start: 81
end: 836
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305777
display id: annotation2305777
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305777/range2305777/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150013/annotation2305777/range2305777/1
type: Range
persistent identity: range2305777
display id: range2305777
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 862
end: 869
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2305778
display id: annotation2305778
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305778/range2305778/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K150013/annotation2305778/range2305778/1
type: Range
persistent identity: range2305778
display id: range2305778
version: 1
top level: BBa_K150013
direction: direction/forward
start: 870
end: 949
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305780
display id: annotation2305780
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305780/range2305780/1
component: BBa_K150100

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type: Range
persistent identity: range2305780
display id: range2305780
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 950
end: 957
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2305781
display id: annotation2305781
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305781/range2305781/1
component: BBa_B0012

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type: Range
persistent identity: range2305781
display id: range2305781
version: 1
top level: BBa_K150013
direction: direction/forward
start: 958
end: 998
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305785
display id: annotation2305785
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305785/range2305785/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150013/annotation2305785/range2305785/1
type: Range
persistent identity: range2305785
display id: range2305785
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 999
end: 1006
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2305787
display id: annotation2305787
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305787/range2305787/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K150013/annotation2305787/range2305787/1
type: Range
persistent identity: range2305787
display id: range2305787
version: 1
top level: BBa_K150013
direction: direction/forward
start: 1007
end: 1061
orientation: inline

BBa_K150104
type: SequenceAnnotation
persistent identity: annotation2305792
display id: annotation2305792
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305792/range2305792/1
component: BBa_K150104

https://synbiohub.org/public/igem/BBa_K150013/annotation2305792/range2305792/1
type: Range
persistent identity: range2305792
display id: range2305792
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 1062
end: 1069
orientation: inline

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation2305794
display id: annotation2305794
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305794/range2305794/1
component: BBa_K150005

https://synbiohub.org/public/igem/BBa_K150013/annotation2305794/range2305794/1
type: Range
persistent identity: range2305794
display id: range2305794
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 1070
end: 1082
orientation: inline

BBa_K150008
type: SequenceAnnotation
persistent identity: annotation2305795
display id: annotation2305795
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305795/range2305795/1
component: BBa_K150008

https://synbiohub.org/public/igem/BBa_K150013/annotation2305795/range2305795/1
type: Range
persistent identity: range2305795
display id: range2305795
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 1083
end: 1088
orientation: inline

BBa_K150012
type: SequenceAnnotation
persistent identity: annotation2305799
display id: annotation2305799
version: 1
top level: BBa_K150013
location: https://synbiohub.org/public/igem/BBa_K150013/annotation2305799/range2305799/1
component: BBa_K150012

https://synbiohub.org/public/igem/BBa_K150013/annotation2305799/range2305799/1
type: Range
persistent identity: range2305799
display id: range2305799
version: 1
top level: BBa_K150013
direction: direction/reverse
start: 1089
end: 2112
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K150100
type: ComponentDefinition
persistent identity: BBa K150100
display id: BBa_K150100
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD00
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: original scar from BioBricks
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150100_sequence/1

BBa_K150107
type: ComponentDefinition
persistent identity: BBa K150107
display id: BBa_K150107
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD07
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: linkerHD07
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K150107_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_K150104
type: ComponentDefinition
persistent identity: BBa K150104
display id: BBa_K150104
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD04
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: DNA linker region between lux pR promoter BBa_R0062 and ribosome binding site pTrc99a (BBa_) for the ColicinE1 Producer Controlled by 3OC6HSL Receiver Device (BBa_).
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: linkerHD04
sequence: https://synbiohub.org/public/igem/BBa_K150104_sequence/1

linkerHD04
type: SequenceAnnotation
persistent identity: annotation1983191
display id: annotation1983191
version: 1
top level: BBa_K150104
location: https://synbiohub.org/public/igem/BBa_K150104/annotation1983191/range1983191/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K150104/annotation1983191/range1983191/1
type: Range
persistent identity: range1983191
display id: range1983191
version: 1
top level: BBa_K150104
direction: direction/forward
start: 1
end: 8
orientation: inline

BBa_K150005
type: ComponentDefinition
persistent identity: BBa K150005
display id: BBa_K150005
version: 1
was generated by: iGEM to SBOL conversion
description: ribosome binding site of pTrc99a
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: ribosome binding site of pTrc99a
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: BBa_K150005
sequence: https://synbiohub.org/public/igem/BBa_K150005_sequence/1

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation1983190
display id: annotation1983190
version: 1
top level: BBa_K150005
location: https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1
type: Range
persistent identity: range1983190
display id: range1983190
version: 1
top level: BBa_K150005
direction: direction/forward
start: 1
end: 13
orientation: inline

BamHI
type: ComponentDefinition
persistent identity: BBa K150008
display id: BBa_K150008
version: 1
was generated by: iGEM to SBOL conversion
description: BamHI restriction enzyme site
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: BamHI restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150008_sequence/1

BBa_K150012
type: ComponentDefinition
persistent identity: BBa K150012
display id: BBa_K150012
version: 1
was generated by: iGEM to SBOL conversion
description: colicinE9 operon (short)
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This colicinE9 operon (short) contains all three important genes for colicinE9 expression and release. The colE9 gene codes for the lethal colicinE9 protein. The gene for the colE9 immunity protein is responsible for the immunity of the producing host bacteria to the colicinE9 and is therefore controlled by a constitutive promoter. Expression of the atypical lysis protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE9.
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: colE9 protein
sequence annotation: colE9 immunity protein
sequence annotation: atypical lysis protein
sequence: https://synbiohub.org/public/igem/BBa_K150012_sequence/1

colE9 protein
type: SequenceAnnotation
persistent identity: annotation1983740
display id: annotation1983740
version: 1
top level: BBa_K150012
location: https://synbiohub.org/public/igem/BBa_K150012/annotation1983740/range1983740/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K150012/annotation1983740/range1983740/1
type: Range
persistent identity: range1983740
display id: range1983740
version: 1
top level: BBa_K150012
direction: direction/forward
start: 1
end: 620
orientation: inline

colE9 immunity protein
type: SequenceAnnotation
persistent identity: annotation1983741
display id: annotation1983741
version: 1
top level: BBa_K150012
location: https://synbiohub.org/public/igem/BBa_K150012/annotation1983741/range1983741/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K150012/annotation1983741/range1983741/1
type: Range
persistent identity: range1983741
display id: range1983741
version: 1
top level: BBa_K150012
direction: direction/forward
start: 622
end: 882
orientation: inline

atypical lysis protein
type: SequenceAnnotation
persistent identity: annotation1983742
display id: annotation1983742
version: 1
top level: BBa_K150012
location: https://synbiohub.org/public/igem/BBa_K150012/annotation1983742/range1983742/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K150012/annotation1983742/range1983742/1
type: Range
persistent identity: range1983742
display id: range1983742
version: 1
top level: BBa_K150012
direction: direction/forward
start: 944
end: 1024
orientation: inline

https://synbiohub.org/public/igem/BBa_K150013_sequence/1
type: Sequence
persistent identity: BBa K150013 sequence
display id: BBa_K150013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaggaaacagaaccggatccatcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccgggcttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgagcttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctccgtttactccaaagaatcaacaggtcggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatatccgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaaaatggaactgaagcatagcattagtgattatacagaagctgaatttttacagcttgtaacaacaatttgtaatgcggacacttccagtgaagaagaactggttaaattggttacacactttgaggaaatgactgagcaccctagtggtagtgatttaatatattacccaaaagaaggtgatgatgactcaccttcaggtattgtaaacacagtaaaacaatggcgagccgctaacggtaagtcaggatttaaacagggctaaaatatgagtgccggttgtttaaggatgaat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150100_sequence/1
type: Sequence
persistent identity: BBa K150100 sequence
display id: BBa_K150100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150107_sequence/1
type: Sequence
persistent identity: BBa K150107 sequence
display id: BBa_K150107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150104_sequence/1
type: Sequence
persistent identity: BBa K150104 sequence
display id: BBa_K150104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150005_sequence/1
type: Sequence
persistent identity: BBa K150005 sequence
display id: BBa_K150005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaaacagaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150008_sequence/1
type: Sequence
persistent identity: BBa K150008 sequence
display id: BBa_K150008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150012_sequence/1
type: Sequence
persistent identity: BBa K150012 sequence
display id: BBa_K150012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccgggcttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgagcttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctccgtttactccaaagaatcaacaggtcggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatatccgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaaaatggaactgaagcatagcattagtgattatacagaagctgaatttttacagcttgtaacaacaatttgtaatgcggacacttccagtgaagaagaactggttaaattggttacacactttgaggaaatgactgagcaccctagtggtagtgatttaatatattacccaaaagaaggtgatgatgactcaccttcaggtattgtaaacacagtaaaacaatggcgagccgctaacggtaagtcaggatttaaacagggctaaaatatgagtgccggttgtttaaggatgaatggctggcattctttcacaacaaggagtcgttatgaaaaaaataacagggattattttattgcttcttgcagtcattattctgtctgcatggggttctaagccgaaaacctag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z