BBa_K1505002 1 BBa_K1505002 RBS+mntH+mCerulean 2014-10-09T11:00:00Z 2016-01-13T10:36:51Z RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. MntH is from part BBa_K1505000. mCerulean is from part BBa_K133066. The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. mntH was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The MntH protein and mCerulean protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of MntH and for better visualization of successful transformation. The original use is to express MntH in Magnetospirillum magneticum strain AMB-1. false false _1885_ 4206 22465 9 In stock false We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCerulean protein is expected to improve the folding of MntH in AMB-1. false Jane Shmushkis, Amey Vrudhula annotation2412329 1 ggggs linker range2412329 1 1303 1317 annotation2412327 1 rbs (from pmsp3) range2412327 1 19 46 annotation2412328 1 mntH range2412328 1 67 1302 annotation2412330 1 mCerulean range2412330 1 1318 2034 BBa_K1505002_sequence 1 gctactggtaccgctactgtccaagccctatgagggagcctttctagctcactgctcttcgctactatgacgaactatcgcgttgagagtagcagcggacgggcggcgcgcaagatgaggctcgcattaatgggacctgcgttcattgcggcgattggttatatcgatcccggtaactttgcgaccaatattcaggcgggtgcaagtttcggctatcagctactgtgggttgtcgtttgggccaacctgatggcgatgctgattcagatcctctctgccaaactagggattgccactggtaaaaatctggcggagcagattcgcgatcactatccgcgtcccgtagtgtggttctattgggttcaggcagaaattattgcgatggcaaccgacctggcggaatttattggtgcggcgatcggttttaaactcattcttggtgtttcgttgttgcagggcgcggtgctgacggggatcgcgactttcctgattttaatgctgcaacgtcgcgggcaaaaaccgctggagaaagtgattggcgggttactgttgtttgttgccgcggcttacattgtcgagttgattttctcccagcctaacctggcgcagctcggtaaaggaatggtgatcccgagtttacctacttcggaagcggtcttcctggcagcaggcgtgttaggggcgacgattatgccgcatgtgatttatttgcactcctcgctcactcagcatttacatggcggttcgcgtcaacaacgttattccgccaccaaatgggatgtggctatcgccatgactattgccggttttgtcaatctggcgatgatggctacagcagcggcggcgttccacttttccggtcatactggtgttgccgatcttgatgaggcttatctgacgctgcaaccgctgttaagccacgctgcggcaacggtctttggattaagcctggttgctgcggggctgtcttcaacggtggtggggacactggcggggcaggtggtgatgcagggcttcattcgctttcatatcccgctgtgggtgcgtcgtacagtcaccatgttgccgccatttattgtcattctgatgggattagatccgacacggattctggttatgagtcaggtactgttaagttttggtatcgctctggcgctggttccactgctgattttcaccagtgacagcaagttgatgggcgatctggtgaacagcaaacgcgtaaaacagacaggctgggtgattgtggtgctggtcgtggcgctgaatatctggttgttggtggggacggcgctgggattgggtggaggcggttcagtgagcaagggtgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z