LOCUS       BBa_K1507014            1816 bp    DNA     linear       16-Feb-2025
DEFINITION  Fatty acid induced expression auto regulator (pfadBA-B0030-C0051-R0
            051-B0034-E0040-B0015)
ACCESSION   BBa_K1507014
VERSION     BBa_K1507014.1  
FEATURES             Location/Qualifiers
     promoter        1..72
                     /label=PfadBA
     promoter        1..72
                     /label=BBa_K817002
     RBS             81..95
                     /label=RBS-1\Strong
     RBS             88..92
                     /label=RBS
     misc_feature    81..95
                     /label=BBa_B0030
     RBS             81..95
                     /label=BBa_B0030
     CDS             105..812
                     /label=cI lambda
     misc_feature    813..845
                     /label=LVA
     misc_feature    852..876
                     /label=Help:Barcodes
     CDS             102..876
                     /label=BBa_C0051
     promoter        922..927
                     /label=-10
     promoter        899..904
                     /label=-35
     misc_feature    909..925
                     /label=OR1
     misc_feature    885..901
                     /label=OR2
     misc_feature    885..933
                     /label=BBa_R0051
     promoter        885..933
                     /label=BBa_R0051
     misc_feature    946..949
                     /label=conserved
     RBS             942..953
                     /label=BBa_B0034
     CDS             960..1679
                     /label=GFP protein
     CDS             960..1679
                     /label=BBa_E0040
     stem_loop       1699..1742
                     /label=stem_loop
     misc_feature    1688..1767
                     /label=BBa_B0010
     terminator      1688..1767
                     /label=BBa_B0010
     stem_loop       1783..1802
                     /label=T7 TE
     misc_feature    1809..1809
                     /label=stop
     polyA_site      1803..1816
                     /label=polya
     misc_feature    1776..1816
                     /label=BBa_B0012
     terminator      1776..1816
                     /label=BBa_B0012
     terminator      1688..1816
                     /label=BBa_B0015
ORIGIN
        1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag
       61 gacgcactga cctactagag attaaagagg agaaatacta gatgagcaca aaaaagaaac
      121 cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga
      181 aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg gggcagtcag
      241 gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc gcattgcttg
      301 caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg
      361 agatgtatga agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt
      421 tttctcatgt tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg
      481 cggagagatg ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg
      541 aaggtaattc catgaccgca ccaacaggct ccaagccaag ctttcctgac ggaatgttaa
      601 ttctcgttga ccctgagcag gctgttgagc caggtgattt ctgcatagcc agacttgggg
      661 gtgatgagtt taccttcaag aaactgatca gggatagcgg tcaggtgttt ttacaaccac
      721 taaacccaca gtacccaatg atcccatgca atgagagttg ttccgttgtg gggaaagtta
      781 tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag
      841 tagcttaata acgctgatag tgctagtgta gatcgctact agagtaacac cgtgcgtgtt
      901 gactatttta cctctggcgg tgataatggt tgctactaga gaaagaggag aaatactaga
      961 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1021 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     1081 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     1141 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
     1201 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1261 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1321 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1381 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1441 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1501 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1561 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1621 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1681 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1741 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1801 cctttctgcg tttata
//