BBa_K1507016
type: ComponentDefinition
persistent identity: BBa K1507016
display id: BBa_K1507016
version: 1
was generated by: iGEM to SBOL conversion
description: Fatty acid induced expression auto regulator (pfadBA-B0034-C0051-pfadBA-B0034-C0051-R0051-B0034-E004
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: Biobricks
owned by: james
owned by: myers
mutable description: A down-counting timer system. The expression of GFP (green fluorescence protein) will be shut down after a period of time whenever the bacteria uptakes fatty acid.
discontinued: false
dominant: false
group u list: _1887_
m user id: 0
owner id: 23158
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No data
star: false
creator: Willy Hsu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K817002
component: BBa_C0051
component: BBa_B0015
component: BBa_E0040
sequence annotation: BBa_K817002
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1507016_sequence/1

BBa_B0034
type: Component
persistent identity: component2400334
display id: component2400334
version: 1
top level: BBa_K1507016
definition: BBa_B0034
access: public

BBa_K817002
type: Component
persistent identity: component2400332
display id: component2400332
version: 1
top level: BBa_K1507016
definition: BBa_K817002
access: public

BBa_C0051
type: Component
persistent identity: component2400330
display id: component2400330
version: 1
top level: BBa_K1507016
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component2400326
display id: component2400326
version: 1
top level: BBa_K1507016
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2400349
display id: component2400349
version: 1
top level: BBa_K1507016
definition: BBa_B0015
access: public

BBa_E0040
type: Component
persistent identity: component2400342
display id: component2400342
version: 1
top level: BBa_K1507016
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2400340
display id: component2400340
version: 1
top level: BBa_K1507016
definition: BBa_B0034
access: public

BBa_C0051
type: Component
persistent identity: component2400338
display id: component2400338
version: 1
top level: BBa_K1507016
definition: cI lam
access: public

BBa_K817002
type: Component
persistent identity: component2400324
display id: component2400324
version: 1
top level: BBa_K1507016
definition: BBa_K817002
access: public

BBa_K817002
type: SequenceAnnotation
persistent identity: annotation2400324
display id: annotation2400324
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400324/range2400324/1
component: BBa_K817002

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400324/range2400324/1
type: Range
persistent identity: range2400324
display id: range2400324
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 1
end: 72
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2400326
display id: annotation2400326
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400326/range2400326/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400326/range2400326/1
type: Range
persistent identity: range2400326
display id: range2400326
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 81
end: 92
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2400330
display id: annotation2400330
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400330/range2400330/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400330/range2400330/1
type: Range
persistent identity: range2400330
display id: range2400330
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 99
end: 873
orientation: inline

BBa_K817002
type: SequenceAnnotation
persistent identity: annotation2400332
display id: annotation2400332
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400332/range2400332/1
component: BBa_K817002

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400332/range2400332/1
type: Range
persistent identity: range2400332
display id: range2400332
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 882
end: 953
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2400334
display id: annotation2400334
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400334/range2400334/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400334/range2400334/1
type: Range
persistent identity: range2400334
display id: range2400334
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 962
end: 973
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2400338
display id: annotation2400338
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400338/range2400338/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400338/range2400338/1
type: Range
persistent identity: range2400338
display id: range2400338
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 980
end: 1754
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2400340
display id: annotation2400340
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400340/range2400340/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400340/range2400340/1
type: Range
persistent identity: range2400340
display id: range2400340
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 1763
end: 1774
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2400342
display id: annotation2400342
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400342/range2400342/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400342/range2400342/1
type: Range
persistent identity: range2400342
display id: range2400342
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 1781
end: 2500
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2400349
display id: annotation2400349
version: 1
top level: BBa_K1507016
location: https://synbiohub.org/public/igem/BBa_K1507016/annotation2400349/range2400349/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1507016/annotation2400349/range2400349/1
type: Range
persistent identity: range2400349
display id: range2400349
version: 1
top level: BBa_K1507016
direction: direction/reverse
start: 2509
end: 2637
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K817002
type: ComponentDefinition
persistent identity: BBa K817002
display id: BBa_K817002
version: 1
was generated by: iGEM to SBOL conversion
description: PfadBA
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:28Z
mutable provenance: de novo synthesis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Fatty acid sensitive promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1075_
m user id: 0
owner id: 13572
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Yi-Te Lee, Chieh-Mei Wang
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PfadBA
sequence: https://synbiohub.org/public/igem/BBa_K817002_sequence/1

PfadBA
type: SequenceAnnotation
persistent identity: annotation2197686
display id: annotation2197686
version: 1
top level: BBa_K817002
location: https://synbiohub.org/public/igem/BBa_K817002/annotation2197686/range2197686/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K817002/annotation2197686/range2197686/1
type: Range
persistent identity: range2197686
display id: range2197686
version: 1
top level: BBa_K817002
direction: direction/forward
start: 1
end: 72
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1507016_sequence/1
type: Sequence
persistent identity: BBa K1507016 sequence
display id: BBa_K1507016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagatctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K817002_sequence/1
type: Sequence
persistent identity: BBa K817002 sequence
display id: BBa_K817002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z