BBa_K1509002
1
BBa_K1509002
Mainly responsible for the flocculation of <em>Bacillus sp. F2</em>
2014-09-25T11:00:00Z
2015-05-08T01:10:47Z
It???s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.
It is responsible for the flocculent ability of Bacillus sp. F2 and has been converted to E.coli JM109. It has been proved that strain of E.coli positive clone FC2 could express flocculent activity, with the flocculent efficiency of 90%, thus can be a good choice in environmental applications. The 1071bp gene consists of 29%T, 18%C, 25%G and 29%A.
false
false
_1889_
0
24032
9
It's complicated
false
It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below:
P1: 5???-AGTCTAGATGTAAGCTCTCTTCCGG-3???
P2: 5???-CTCTCGAGCAATAAGGACGCAATG-3???
And the PCR runs under the settings as follows:
Temperature Number of cycle
96℃; 15min 1
94℃; 40sec 30
50℃; 60sec
72℃; 4min
72℃; 7min 1
Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.
false
Tong Li
annotation2387082
1
BioBrick
range2387082
1
1
1038
BBa_K1509002_sequence
1
atgagtctacttgctgttttgtttttagtggcgtgtggaggtaagtctgaaaagacagctacatcttcatcatctacgacttcttcttcatcagaagaagctgtttcaggtgcatctgagaaagtttatacagatccatctgagttgaaagatagctacgatgttatcgttgttggttcaggtggtgcaggtatgtcagcagctatctcagctaaagacgctggtgctagtgttgctctcttggagaaaatgcctgttatcggtggtaatacggctaaatcatcagctggtacgaatgcttcacagactaaattccaagaagcggaaggtattgctgatacaaatgataaattctacgaagaaacgcttaaaggtggtaaagggacaaacgatcctgaattgcttcgttatcttgtagataactctgctagtgcaattgactggttggatggaatggggatcactcttagcaacctaactacaactggtggtatgagcgagaaacgtacccaccgtccagcagatggttcagctgtcggtggctacttggtaaatggtctttaccacaatcttgttgaacgtgaagtaccaatttttgtcaatgcagatgttacaaaactccaagacaaggatggagctgtgacaggtgttgaagtgaagattgctggtgaaactaagaaaatctcagctaaagcagttgtattggcgactggtggtttcggtgctgatttggaaatggttgctaaattaaacccagcattgaaaggttatgttacaactaaccaagaaggatcaacaggtgacggtattgcgcttgctgaatcagaaggtgcagcaactgttgatatggaacaaatccaaattcacccaagtgttgagcaagaaacttcttacttgattacagaagcagttcgtggcgaaggtgctatccttgtcaactcaaaaggtgagcgttttgttaacgaattggaaactcgtgataaagtttctgcggctatcaatagtttggatccccgggtaccgagctcgaattcgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z