BBa_K1510002 1 BBa_K1510002 PnlmC (S.mutans) 2014-10-05T11:00:00Z 2015-05-08T01:10:47Z Streptococcus mutans UA159 This part is derived from Streptococcus mutans UA159, a gram positive bacterium. The main function of this promoter is to sense a quorum sensing chemical, competence-stimulating peptide (CSP), and trigger down string gene. false false _1890_ 0 21272 9 In stock false Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS. false Yen-An Chang annotation2425447 1 -10 extend region range2425447 1 69 76 annotation2425417 1 comE binding site range2425417 1 32 42 annotation2424695 1 comE binding site range2424695 1 11 21 annotation2425446 1 -35 extend region range2425446 1 48 53 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1510004 1 BBa_K1510004 Pcon(S.mutans)+GFP+terminator (S.mutans) 2014-10-05T11:00:00Z 2015-05-08T01:10:47Z Streptococcus mutans UA159+BBa_E0040 (2014 iGEM kit) This part is composed of constitutive promoter, RBS, GFP and terminaor. Constitutive promoter, RBS and terminator are derived from Streptococcus mutans UA159, and GFP is from 2014 iGEM kit (BBa_E0040). The main function of this part is to trigger reporter gene continually, and can be served as a reporter of transformation efficiency in both Streptococcus mutans and E.coli. false false _1890_ 0 21272 9 Not in stock false To assemble these parts together, gibson assembly are used. false Yen-An Chang component2396721 1 BBa_E0040 component2396719 1 BBa_K1510002 annotation2396719 1 BBa_K1510002 range2396719 1 1 136 annotation2396721 1 BBa_E0040 range2396721 1 143 862 BBa_K1510002_sequence 1 atcaaaaatgaccgtttaggacaaaatagctaccatttaggatattttgctccattttgaaaataaattgttatactagagatgtcggttgcgcagccagacaaaaaactaaaaatggggaaggggtatttatcat BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1510004_sequence 1 atcaaaaatgaccgtttaggacaaaatagctaccatttaggatattttgctccattttgaaaataaattgttatactagagatgtcggttgcgcagccagacaaaaaactaaaaatggggaaggggtatttatcattactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z