BBa_K1510002
1
BBa_K1510002
PnlmC (S.mutans)
2014-10-05T11:00:00Z
2015-05-08T01:10:47Z
Streptococcus mutans UA159
This part is derived from Streptococcus mutans UA159, a gram positive bacterium. The main function of this promoter is to sense a quorum sensing chemical, competence-stimulating peptide (CSP), and trigger down string gene.
false
false
_1890_
0
21272
9
In stock
false
Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS.
false
Yen-An Chang
annotation2425447
1
-10 extend region
range2425447
1
69
76
annotation2425417
1
comE binding site
range2425417
1
32
42
annotation2424695
1
comE binding site
range2424695
1
11
21
annotation2425446
1
-35 extend region
range2425446
1
48
53
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1510004
1
BBa_K1510004
Pcon(S.mutans)+GFP+terminator (S.mutans)
2014-10-05T11:00:00Z
2015-05-08T01:10:47Z
Streptococcus mutans UA159+BBa_E0040 (2014 iGEM kit)
This part is composed of constitutive promoter, RBS, GFP and terminaor. Constitutive promoter, RBS and terminator are derived from Streptococcus mutans UA159, and GFP is from 2014 iGEM kit (BBa_E0040). The main function of this part is to trigger reporter gene continually, and can be served as a reporter of transformation efficiency in both Streptococcus mutans and E.coli.
false
false
_1890_
0
21272
9
Not in stock
false
To assemble these parts together, gibson assembly are used.
false
Yen-An Chang
component2396721
1
BBa_E0040
component2396719
1
BBa_K1510002
annotation2396719
1
BBa_K1510002
range2396719
1
1
136
annotation2396721
1
BBa_E0040
range2396721
1
143
862
BBa_K1510002_sequence
1
atcaaaaatgaccgtttaggacaaaatagctaccatttaggatattttgctccattttgaaaataaattgttatactagagatgtcggttgcgcagccagacaaaaaactaaaaatggggaaggggtatttatcat
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1510004_sequence
1
atcaaaaatgaccgtttaggacaaaatagctaccatttaggatattttgctccattttgaaaataaattgttatactagagatgtcggttgcgcagccagacaaaaaactaaaaatggggaaggggtatttatcattactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z