BBa_K1510101
1
BBa_K1510101
sRNA targets histidine kinase 11 mRNA in S.mutans
2014-10-05T11:00:00Z
2015-05-08T01:10:48Z
primer
sRNA targets histidine kinase 11 in S.mutans
false
false
_1890_
0
21299
9
Not in stock
false
complementary to histidine kinase 11
false
Wei-Tai Chen
BBa_K1510100
1
BBa_K1510100
constitutive promoter (veg promoter) in S.mutans
2014-10-05T11:00:00Z
2015-05-08T01:10:48Z
streptococcus mutans
constitutive promoter (veg promoter) in S.mutans
false
false
_1890_
0
21299
9
Not in stock
false
To achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)
false
Wei-Tai Chen
annotation2396754
1
EcoRI site
range2396754
1
4
9
BBa_K1510103
1
BBa_K1510103
MicC scaffold from E.coli MG1655
2014-10-05T11:00:00Z
2015-05-08T01:10:48Z
E.coli MG1655
MicC scaffold from E.coli MG1655, it is a RNA sequence and can recruit Hfq protein in sRNA gene knockdown mechanism.
false
false
_1890_
0
21299
9
It's complicated
false
No
false
Wei-Tai Chen
BBa_K1510104
1
BBa_K1510104
terminator in S.mutans
2014-10-05T11:00:00Z
2015-05-08T01:10:48Z
primer
terminator in S.mutans
false
false
_1890_
0
21299
9
Not in stock
false
It has BamHI cutting cite in the last
false
Wei-Tai Chen
annotation2396768
1
BamHI cutting site
range2396768
1
14
19
BBa_K1510006
1
BBa_K1510006
sRNA targets histidine kinase 11 in S.mutans
2014-10-05T11:00:00Z
2015-05-08T01:10:47Z
constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.
Other parts are synthesized by primer.
veg promoter(constitutive promoter in S.mutans+ Histidine kinase 11 sRNA+ MicC scaffold+ terminator. Constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer.
This part will transcibe into sRNA, and sRNA will bind to mRNA of HK11, prevent HK11 mRNA from translating. Because histidine kinase 11 is a biofilm formation-related protein, this part is created to decrease biofilm formation.
false
false
_1890_
0
21299
9
Not in stock
false
Because we need to put our part into vector PVA838 to shuttle plasmid between E.coli and S.mutans. The restriction site before part is EcoRI and BamHI in the last. In addition, to achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)
false
Wei-Tai Chen
component2396767
1
BBa_K1510104
component2396766
1
BBa_K1510103
component2396765
1
BBa_K1510101
component2396764
1
BBa_K1510100
annotation2396766
1
BBa_K1510103
range2396766
1
91
169
annotation2396764
1
BBa_K1510100
range2396764
1
1
50
annotation2396765
1
BBa_K1510101
range2396765
1
59
82
annotation2396767
1
BBa_K1510104
range2396767
1
178
199
BBa_K1510100_sequence
1
ccggaattcttgacaaaaatgggctcgtgttgtacaataaatgtaatgca
BBa_K1510101_sequence
1
agcggttaaaattaaagtccacat
BBa_K1510103_sequence
1
tttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttt
BBa_K1510104_sequence
1
cagctgatagctgggatccaca
BBa_K1510006_sequence
1
ccggaattcttgacaaaaatgggctcgtgttgtacaataaatgtaatgcatactagagagcggttaaaattaaagtccacattactagagtttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagcagctgatagctgggatccaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z