BBa_K1510011 1 BBa_K1510011 Extracellular endolysin 2014-10-05T11:00:00Z 2015-05-08T01:10:48Z J23100 is a strong constitutive promoter from 2014 igem distribution. The coding region, yebF and endolysin come from different origin. YebF is from E.coli K12, while endolysin is from the 19th opening reading frame of Streptococcus Mutans phage M102. Finally, B0015 is a double terminator from igem distribution. By putting strong constitutive promoter, J23100 in front of yebF and its ribosomal biding site. The recombinant endolysin should be secreted. By adding this part, we are able to have recombinant endolysin form Phage M!02 outside E.coli. In our circuit, we use this circuit to see if endolysing from specific phage can be expressed by E.coli and successfully kill Strepptococcus Mutans. false false _1890_ 0 21268 9 It's complicated false The endolysin sequence from phage M102 contains a Spe1 cutting site, therefore, we design primers to make point mutation of the sequence in order to produce the same amino acid without the risk of being cut during circuit construction. false Chien-Yung Huang BBa_K1510011_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagggagaaaaacatgaaaaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtactagaacctcgctaaaaaaaggtgattattttatcgacgtaagtggctatcaacccgcagatttgcacggggtcttctctgcttctggcactaataaaacgataatcaaactgactgagtcgactggttattataacaccagcgcccctagtcaagttgcaacaagcgattgcattggattttaccatttcgcacgatttgggggctctgtggcacaagcgcaaacagaagcgaacttctttcttagcacactagctaagacagacattaaatatttagtctgtgactatgaagactctgcgagcggtgataagcaagcaaacactaatgctgtattagcgtttatggacgtaatcgcaagcgctggctataagcctatatactactcatatagaccatttacgctggaaaatatctatcacgaccagctaaacgccaagtatccaaatagcacttggatagcagcatatccaaactatgcagtaacgcctgaccccgtttggtcaatattcccgtcaatcgacggagctagatggtggcaattcaccagcacagcgattgctagtggacttgataaaaatgtggtgctgttagatgatgatggcgcagtagacgaaattttgaaaggactagaaaacgacatggattacacaattagagaaaaaacaggaaaatatccctacatggtcttgactcatggctccgctgtagcagtagggcatattgacactatctcagcgtttcaaagcgcaggggctaaagacctcaatctcgacacagacgactatcaacgtttgattgcagtaattaacaaaggaaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z