BBa_K1510105
1
BBa_K1510105
sRNA targets histidine kinase 11 mRNA in S.mutans
2014-10-05T11:00:00Z
2015-05-08T01:10:48Z
Veg promoter+HK11 sRNA+MicC scaffold+terminator
veg promoter(constitutive promoter in S.mutans+ Histidine kinase 11 sRNA+ MicC scaffold+ terminator. Constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer.
This part will transcibe into sRNA, and sRNA will bind to mRNA of HK11, prevent HK11 mRNA from translating. Because histidine kinase 11 is a biofilm formation-related protein, this part is created to decrease biofilm formation.
false
false
_1890_
0
21299
9
In stock
false
Because we need to put our part into vector PVA838 to shuttle plasmid between E.coli and S.mutans. The restriction site before part is EcoRI and BamHI in the last. In addition, to achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)
false
Wei-Tai Chen
annotation2396864
1
sRNA targets histidine kinase 11
range2396864
1
42
65
annotation2396865
1
MicC sacffold
range2396865
1
66
144
annotation2396863
1
Veg promoter
range2396863
1
1
36
annotation2396866
1
terminator
range2396866
1
145
157
BBa_K1510105_sequence
1
ttgacaaaaatgggctcgtgttgtacaataaatgtaatgcaagcggttaaaattaaagtccacattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggctttttttcagctgatagctg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z