LOCUS       BBa_K1510214            2053 bp    DNA     linear       25-Nov-2024
DEFINITION  Dispersin generator circuit
ACCESSION   BBa_K1510214
VERSION     BBa_K1510214.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    44..584
                     /label=BBa_K1510009
     stem_loop       1815..1858
                     /label=stem_loop
     misc_feature    1804..1883
                     /label=BBa_B0010
     terminator      1804..1883
                     /label=BBa_B0010
     stem_loop       1899..1918
                     /label=T7 TE
     misc_feature    1925..1925
                     /label=stop
     polyA_site      1919..1932
                     /label=polya
     misc_feature    1892..1932
                     /label=BBa_B0012
     terminator      1892..1932
                     /label=BBa_B0012
     terminator      1804..1916
                     /label=BBa_B0015
     promoter        655..660
                     /label=Sigma A-35
     promoter        678..683
                     /label=Sigma A -10
     misc_feature    593..689
                     /label=BBa_K143012
     RBS             698..709
                     /label=rbs
     misc_feature    698..709
                     /label=BBa_K143021
     misc_feature    718..1803
                     /label=BBa_K391006
     misc_feature    1804..1916
                     /label=BBa_B0015
     misc_feature    593..1916
                     /label=BBa_K802001
     stem_loop       1936..1979
                     /label=stem_loop
     misc_feature    1925..2004
                     /label=BBa_B0010
     terminator      1925..2004
                     /label=BBa_B0010
     stem_loop       2020..2039
                     /label=T7 TE
     misc_feature    2046..2046
                     /label=stop
     polyA_site      2040..2053
                     /label=polya
     misc_feature    2013..2053
                     /label=BBa_B0012
     terminator      2013..2053
                     /label=BBa_B0012
     terminator      1925..2053
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagttgacgg ctagctcagt
       61 cctaggtaca gtgctagcta ctagggagaa aaacatgaaa aaaagagggg cgtttttagg
      121 gctgttgttg gtttctgcct gcgcatcagt tttcgctgcc aataatgaaa ccagcaagtc
      181 ggtcactttc ccaaagtgtg aagatctgga tgctgccgga attgccgcga gcgtaaaacg
      241 tgattatcaa caaaatcgcg tggcgcgttg ggcagatgat caaaaaattg tcggtcaggc
      301 cgatcccgtg gcttgggtca gtttgcagga cattcagggt aaagatgata aatggtcagt
      361 accgctaacc gtgcgtggta aaagtgccga tattcattac caggtcagcg tggactgcaa
      421 agcgggaatg gcggaatatc agcggcgtac tagagccagg catcaaataa aacgaaaggc
      481 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      541 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agaattttgt
      601 caaaataatt ttattgacaa cgtcttatta acgttgatat aatttaaatt ttatttgaca
      661 aaaatgggct cgtgttgtac aataaatgta aaggtggtga aatgaaaaaa gcaattactt
      721 tatttacatt attatgcgcg gtacttctct cattcagtac cgcaacttat gcaaacgcta
      781 tggacttacc taaaaaagaa agcggtctga cgttagatat cgcacgtcgt ttctataccg
      841 ttgatacgat aaaacaattt atcgatacga ttcatcaggc gggcggcact tttctgcatt
      901 tacatttttc cgatcacgag aattatgcat tggaaagttc ttatttggaa caacgagaag
      961 aaaatgcgac cgagaaaaac ggaacctatt tcaatccgaa aacaaataag ccgtttctca
     1021 cttataaaca gctcaatgaa attatctatt atgccaaaga acgaaatatt gaaattgtgc
     1081 ctgaagtcga tagcccgaat catatgacgg cgatttttga tcttttaacc cttaagcacg
     1141 gaaaggaata cgtaaaaggg ctaaaatcgc cttatatcgc cgaggaaatc gatattaata
     1201 accccgaagc ggttgaagtt attaaaacct taatcggtga agtgatctat attttcggac
     1261 attcaagccg gcatttccat atcggcggag atgaatttag ctatgcggtc gaaaataatc
     1321 atgaatttat tcggtatgtg aataccttaa atgattttat caattccaaa gggctaatta
     1381 cccgtgtttg gaatgacggt ttgatcaaaa acaacttaag cgaactcaat aaaaacattg
     1441 aaatcactta ctggagctac gacggtgacg ctcaagccaa agaagatatt caatatcgac
     1501 gtgaaataag agccgatttg cccgaactgc tggcaaacgg ttttaaggtt ttaaactata
     1561 attcttatta tttatacttt gtgcctaaat ccggttctaa tattcacaat gacggtaaat
     1621 atgcggctga agacgtatta aataactgga cattaggtaa atgggacgga aaaaacagct
     1681 caaatcacgt acaaaatacg cagaatatta tcggttcttc tttgtcgatt tggggggaac
     1741 gttccagcgc attaaatgaa caaactattc agcaagcctc taaaaattta ttaaaagcgg
     1801 tgatccaaaa aactaatgat ccgaaatcgc attagccagg catcaaataa aacgaaaggc
     1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcttact
     1921 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1981 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2041 ttctgcgttt ata
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