BBa_K1510214 1 BBa_K1510214 Dispersin generator circuit 2014-10-07T11:00:00Z 2016-02-12T09:00:45Z The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction.The dispersin is from Lyon INSA igem team. Finally, the terminator B0015 is from 2014 igem distribution. he Dispersin gene codes for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram negative bacteria.With strong constitutive promoter,together with ribosomal binding site and signal sequence yebF, make dispersin work more accurately. false false _1890_ 4206 21271 9 It's complicated false To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part. false Kao Jung Chang component2404706 1 BBa_B0015 component2404707 1 BBa_K802001 component2404714 1 BBa_B0015 component2404698 1 BBa_J23100 component2404699 1 BBa_K1510009 annotation2404699 1 BBa_K1510009 range2404699 1 44 584 annotation2404707 1 BBa_K802001 range2404707 1 593 1916 annotation2404698 1 BBa_J23100 range2404698 1 1 35 annotation2404706 1 BBa_B0015 range2404706 1 1804 1916 annotation2404714 1 BBa_B0015 range2404714 1 1925 2053 BBa_K802001 1 BBa_K802001 Dispersin generator for <i>B. subtilis</i> 2012-09-19T11:00:00Z 2016-02-12T09:05:08Z Dispersin is produced by Aggregatibacter actinomycetemcomitans. The part contains the DspB gene coding for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram-negative bacteria. false false _1058_ 4206 13266 9 It's complicated true Used a strong RBS in order to produce high levels of protein. false Remi Hocq, Audrey Masi annotation2203894 1 BBa_B0015 range2203894 1 1212 1324 annotation2188644 1 BBa_K391006 range2188644 1 126 1211 annotation2188642 1 rbs range2188642 1 106 117 annotation2188639 1 Sigma A-35 range2188639 1 63 68 annotation2188640 1 Sigma A -10 range2188640 1 86 91 annotation2188643 1 BBa_K143021 range2188643 1 106 117 annotation2188641 1 BBa_K143012 range2188641 1 1 97 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1510009 1 BBa_K1510009 E.coli Signal sequence YebF 2014-10-05T11:00:00Z 2015-05-08T01:10:48Z The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction. Finally, the terminator B0015 is from 2014 igem distribution. This part is composed with a strong promoter, together with ribosomal binding site and signal sequence yebF, finally a double terminator. As a signal protein, YebF would be secreted outside E.coli. The role of the circuit in our project is basically a control circuit. By contrasting the result of this circuit and the other circuit, containing functional protein, we can assume if the protein works more accurately. false false _1890_ 0 21268 9 It's complicated false To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part. false Chien-Yung Huang BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K802001_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtaaaggtggtgaaatgaaaaaagcaattactttatttacattattatgcgcggtacttctctcattcagtaccgcaacttatgcaaacgctatggacttacctaaaaaagaaagcggtctgacgttagatatcgcacgtcgtttctataccgttgatacgataaaacaatttatcgatacgattcatcaggcgggcggcacttttctgcatttacatttttccgatcacgagaattatgcattggaaagttcttatttggaacaacgagaagaaaatgcgaccgagaaaaacggaacctatttcaatccgaaaacaaataagccgtttctcacttataaacagctcaatgaaattatctattatgccaaagaacgaaatattgaaattgtgcctgaagtcgatagcccgaatcatatgacggcgatttttgatcttttaacccttaagcacggaaaggaatacgtaaaagggctaaaatcgccttatatcgccgaggaaatcgatattaataaccccgaagcggttgaagttattaaaaccttaatcggtgaagtgatctatattttcggacattcaagccggcatttccatatcggcggagatgaatttagctatgcggtcgaaaataatcatgaatttattcggtatgtgaataccttaaatgattttatcaattccaaagggctaattacccgtgtttggaatgacggtttgatcaaaaacaacttaagcgaactcaataaaaacattgaaatcacttactggagctacgacggtgacgctcaagccaaagaagatattcaatatcgacgtgaaataagagccgatttgcccgaactgctggcaaacggttttaaggttttaaactataattcttattatttatactttgtgcctaaatccggttctaatattcacaatgacggtaaatatgcggctgaagacgtattaaataactggacattaggtaaatgggacggaaaaaacagctcaaatcacgtacaaaatacgcagaatattatcggttcttctttgtcgatttggggggaacgttccagcgcattaaatgaacaaactattcagcaagcctctaaaaatttattaaaagcggtgatccaaaaaactaatgatccgaaatcgcattagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct BBa_K1510009_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagggagaaaaacatgaaaaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1510214_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagttgacggctagctcagtcctaggtacagtgctagctactagggagaaaaacatgaaaaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtaaaggtggtgaaatgaaaaaagcaattactttatttacattattatgcgcggtacttctctcattcagtaccgcaacttatgcaaacgctatggacttacctaaaaaagaaagcggtctgacgttagatatcgcacgtcgtttctataccgttgatacgataaaacaatttatcgatacgattcatcaggcgggcggcacttttctgcatttacatttttccgatcacgagaattatgcattggaaagttcttatttggaacaacgagaagaaaatgcgaccgagaaaaacggaacctatttcaatccgaaaacaaataagccgtttctcacttataaacagctcaatgaaattatctattatgccaaagaacgaaatattgaaattgtgcctgaagtcgatagcccgaatcatatgacggcgatttttgatcttttaacccttaagcacggaaaggaatacgtaaaagggctaaaatcgccttatatcgccgaggaaatcgatattaataaccccgaagcggttgaagttattaaaaccttaatcggtgaagtgatctatattttcggacattcaagccggcatttccatatcggcggagatgaatttagctatgcggtcgaaaataatcatgaatttattcggtatgtgaataccttaaatgattttatcaattccaaagggctaattacccgtgtttggaatgacggtttgatcaaaaacaacttaagcgaactcaataaaaacattgaaatcacttactggagctacgacggtgacgctcaagccaaagaagatattcaatatcgacgtgaaataagagccgatttgcccgaactgctggcaaacggttttaaggttttaaactataattcttattatttatactttgtgcctaaatccggttctaatattcacaatgacggtaaatatgcggctgaagacgtattaaataactggacattaggtaaatgggacggaaaaaacagctcaaatcacgtacaaaatacgcagaatattatcggttcttctttgtcgatttggggggaacgttccagcgcattaaatgaacaaactattcagcaagcctctaaaaatttattaaaagcggtgatccaaaaaactaatgatccgaaatcgcattagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z