BBa_K592006
1
BBa_K592006
FixK2 promoter
2011-09-16T11:00:00Z
2015-05-08T01:12:48Z
FixK2 promoter omes from the genome of Bradyrhizobium japonicum.
Released HQ 2013
FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
false
false
_763_
0
7929
9
In stock
false
The sequence information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
false
Lei Sun
annotation2130179
1
FixK2 promoter
range2130179
1
1
250
BBa_K145151
1
ccdB
ccdB coding region
2008-08-06T11:00:00Z
2015-07-08T03:14:50Z
P1010
Released HQ 2013
Coding region for the ccdB (control of cell death) gene.
true
false
_257_
4206
2970
9
In stock
true
just one stop codon in the end
true
Jonas Demeulemeester
annotation1970252
1
stop
range1970252
1
304
306
annotation1970253
1
cds
range1970253
1
1
303
annotation1970251
1
start
range1970251
1
1
3
BBa_K1510233
1
BBa_K1510233
A blue light regulated ccdb apoptosis gene
2014-10-06T11:00:00Z
2015-05-08T01:10:48Z
The blue light promoter was derived from igem kit : K592006. And the ccdb gene was K145151.
This is a composite of two units, a blue light promoter and a ccdb killing gene. The blue light promoter may turn on the transcription of ccdb. And what ccdb does, is to generate DNA binding sequences which led to DNA polymerase dysfunction. And gradually leads to cell death caused by mitosis failure.
false
false
_1890_
0
21271
9
It's complicated
false
Whether the blue light promoter is strong enough to turn on enough ccdb gene, and whether ccdb efficiency greater than cell division ability.
false
Kao Jung Chang
component2404332
1
BBa_K145151
component2404328
1
BBa_K592006
annotation2404332
1
BBa_K145151
range2404332
1
257
562
annotation2404328
1
BBa_K592006
range2404328
1
1
250
BBa_K145151_sequence
1
atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
BBa_K1510233_sequence
1
gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcacatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
BBa_K592006_sequence
1
gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z